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Showing: 261 - 270 of 406 results

  2. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #216246
    Purpose
    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration
    Depositor
    Elina Ikonen
    Article
    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:
    Insert
    Cas9 and sgAAVS1.5
    Use
    CRISPR; Human safe harbor locus (a avs1) site-spe…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216246-plasmid-map-sequence-id-426842

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  7. pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid
    #212830
    Purpose
    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome.
    Depositor
    Wolfgang Enard
    Article
    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:
    Insert
    ZIM3-KRAB-dCas9-P2A-mCherry
    Use
    Tags
    HA-tag and ZIM3-KRAB
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG, TetOn
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212830-plasmid-map-sequence-id-434915

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  8. pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid
    #212831
    Purpose
    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.
    Depositor
    Wolfgang Enard
    Article
    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:
    Insert
    ZIM3-KRAB-dCas9-P2A-mCherry
    Use
    Tags
    HA-tag and ZIM3-KRAB
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG, TetOn
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212831-plasmid-map-sequence-id-434913

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  10. pMH012_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid
    #179435
    Purpose
    Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    TRE-cit-coreHS4-mch (SC)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179435-plasmid-map-sequence-id-355551

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Showing: 261 - 270 of 406 results