2,293 results

1. CMV-mEGFP(A206K)-paCaMKII-ShadowG

   Plasmid

   #167475

   Purpose

   Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII-ShadowG
   

   Tags

   ShadowG and mEGFP(A206K)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-synp-F-synaptophysin-jRGECO1a

   Plasmid

   #102997

   Purpose

   Expresses synaptophysin fused jRGECO1a under the synapsin promoter

   Depositor

   David Cox

   Insert

   Synaptophysin-jRGECO1a
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZac2.1 GfaABC1D rM3D mCherry SV40

   Plasmid

   #92285

   Purpose

   AAV DREADD expression

   Depositor

   Baljit Khakh

   Article

   Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

   Insert

   rM3D
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   GfaABC1D

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. bactin:GFP-LAP2b

   Plasmid

   #105980

   Purpose

   transgenesis, ubiquitous expression

   Depositor

   Caren Norden

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Insert

   LAP2b
   

   Tags

   GFP

   Expression

   Bacterial

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. G protein alpha-s-GFP

   Plasmid

   #66083

   Purpose

   G protein alpha-s internally tagged with EGFP and EE epitope

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

   Insert

   G-alpha-s-EE-EGFP (Gnas Rat)
   

   Tags

   EGFP flanked by SGGGS on each side was inserted i…

   Expression

   Mammalian

   Mutation

   BamHI sites in the alpha-s cDNA were removed and …

   Promoter

   CMV

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBiFC-bFOSDelataZipVC155

   Plasmid

   #22014

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   bFosDelataZIPVC155
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deletion of residues 179-193 in bFos(118-211)

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-AU1-mTOR-R2505P

   Plasmid

   #26038

   Depositor

   Fuyuhiko Tamanoi

   Article

   Sato et al Oncogene. 2010 May 6. 29(18):2746-52.

   Insert

   mammalian target of rapamycin-R2505P (Mtor Rat)
   

   Tags

   AU1

   Expression

   Mammalian

   Mutation

   R2505P

   Available Since

   Sept. 21, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

   Plasmid

   #50943

   Purpose

   Bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

   Depositor

   Tobias Bonhoeffer, Mark Huebener, Tobias Rose

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Insert

   mRuby2-P2A-GCaMP6f
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-AAV-syn-jGCaMP7b-WPRE

   Plasmid

   #104489

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV Retrograde and AAV1

   Insert

   jGCaMP7b
   

   Use

   AAV

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt

    Plasmid

    #175180

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    pAAV hSyn FLEX iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Promoter

    human Synapsin

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGFP Cas

    Plasmid

    #50729

    Purpose

    mammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    Cas (Bcar1 Rat)
    

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-Dynamin 2 K44A

    Plasmid

    #22301

    Depositor

    Pietro De Camilli

    Article

    Ochoa et al J Cell Biol. 2000 Jul 24. 150(2):377-89.

    Insert

    Dynamin 2 K44A (Dnm2 Rat)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    K44A

    Available Since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pAAV ORANGE Dlg4-HaloTag KI

    Plasmid

    #139656

    Purpose

    Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and HaloTag donor
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. EBFP2-Cx26

    Plasmid

    #69021

    Purpose

    Expresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Gja2 (Gjb2, Cx26 Rat)
    

    Tags

    EBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-myc-Wnk1

    Plasmid

    #38779

    Depositor

    Melanie Cobb

    Article

    Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

    Insert

    Wnk1 (Wnk1 Rat)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

16. mPA-GFP-Lysosomes-20

    Plasmid

    #57143

    Purpose

    Localization: Lysosome Membrane, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    LAMP1
    

    Tags

    mPA-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pcDNA3 barr1 flag

    Plasmid

    #14687

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    beta arrestin 1 (Arrb1 Rat)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

18. mApple-Lysosomes-20

    Plasmid

    #54921

    Purpose

    Localization: Lysosome Membrane, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    LAMP1
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pN1-RnKIF5C(1-560)-Halo-Flag

    Plasmid

    #188071

    Purpose

    Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags

    Depositor

    Kristen Verhey

    Article

    Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

    Insert

    rat KIF5C (Kif5c Rat)
    

    Tags

    Flag and Halo

    Expression

    Mammalian

    Mutation

    aa1-560

    Promoter

    CMV

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-rat PKA-RIIbeta-mEGFP

    Plasmid

    #45523

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA regulatory subunit RII beta tagged by mEGFP
    

    Expression

    Mammalian

    Available Since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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