We narrowed to 1,246 results for: LIPI

1. pBEL1161

   Plasmid

   #196026

   Purpose

   Expression/purification of 6xHis-TEV-MlaDdelta141-183

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   MlaD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-TEV-MlaDdelta141-183

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBEL1195

   Plasmid

   #196027

   Purpose

   Complementation of MlaF-MlaE-MlaD-MlaC-MlaB

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   MlaFEDCB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. UG75-CDS1-3HA

   Plasmid

   #184760

   Purpose

   CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   CDS1 (CDS1 Budding Yeast)
   

   Use

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   CDS1

   Available since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Soat2_Luc

   Plasmid

   #177623

   Purpose

   Cloned human Soat2 promoter containing exon1 and 2 coupled to luciferase

   Depositor

   Thomas Burris

   Article

   Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

   Insert

   human Soat2
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. NmMetNI-WT

   Plasmid

   #172114

   Purpose

   Expression of MetN and MetI membrane proteins

   Depositor

   Douglas Rees

   Article

   Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

   Inserts

   MetN
   MetI
   

   Use

   Tags

   10x His Tag and Enterokinase

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. GST-OPTNS177DS473D

   Plasmid

   #171425

   Purpose

   For the purifcation of phosphorylation mimic protein

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   OPTN (OPTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   phosphorylation mutant (S177D, S473D)

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. GST-TEVcs- FIP200 delta641-779-MBP

   Plasmid

   #171411

   Purpose

   For the purifcation of human ULK1 truncated complex

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   FIP200 (RB1CC1 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   truncation (delta641-779-MBP)

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-Metavinculin tail

   Plasmid

   #107152

   Purpose

   bacterial expression

   Depositor

   Tina Izard

   Article

   Chinthalapudi et al Proc Natl Acad Sci U S A. 2016 Aug 23;11

   Insert

   metavinculin (VCL Human)
   

   Use

   Tags

   (His)8

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJexpress414_OmpA171_WTnoGirdles

   Plasmid

   #62653

   Purpose

   Wild-type OmpA with aromatic girdle residues replaced by leucine

   Depositor

   Timothy Whitehead

   Article

   Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

   Insert

   OmpA_noGirdles
   

   Use

   Synthetic Biology

   Tags

   6His and FlAsH

   Expression

   Bacterial

   Mutation

   Aromatic girdle residues mutated to Leu (W7, W15,…

   Promoter

   T7

   Available since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pRSETC-PITPbeta (splice variant 1) K61A

    Plasmid

    #58271

    Purpose

    bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

    Depositor

    Shamshad Cockcroft

    Article

    Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

    Insert

    PITP beta (PITPNB Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pET-NLS-Cas9-6xHis

    Plasmid

    #62934

    Purpose

    Expression of NLS-Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    NLS-Cas9-6xHis
    

    Use

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-Cas9-6xHis

    Plasmid

    #62374

    Purpose

    Expression of Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9_6xHis
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCit(P5f-P13f)3-gs

    Plasmid

    #228080

    Purpose

    Mammalian expression of coiled coil segments (P5f-P13f)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    mCit, P5f, P13f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. TagBFP(P6f)3(P14f)3-gs

    Plasmid

    #228081

    Purpose

    Mammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers and N-term TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    BFP, P6f, P14f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. FAB(ER-mito)

    Plasmid

    #214427

    Purpose

    Detection of ER-mito contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    FRB-V5
    

    Use

    Tags

    CFAST10-Halo and RspAN-FRB-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. iRFP-Sec61beta

    Plasmid

    #108125

    Purpose

    ER marker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    iRFP:SEC61B (SEC61B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-PM-ExRai-AktAR2

    Plasmid

    #184050

    Purpose

    Plasma membrane-targeted ExRai-AktAR2

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    PM-ExRai-AktAR2
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXJ-Myc-IRF1

    Plasmid

    #232641

    Purpose

    transient expresses IRF1 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

    Insert

    IRF1 (IRF1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-PASS

    Plasmid

    #193970

    Purpose

    Monitoring the production of phosphatidic acid with GFP-PASS (phosphatidic acid biosensor with superior sensitivity)

    Depositor

    Guangwei Du

    Article

    Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

    Insert

    PA biosenor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-C1-PLCdelta-PH

    Plasmid

    #21179

    Purpose

    encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

    Depositor

    Tobias Meyer

    Article

    Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

    Insert

    PLCdelta (PLCD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PH domain of human PLC delta, amino acids Met1–Il…

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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