We narrowed to 7,101 results for: tev

1. pAG8Ha-FLI1_DBD_deltaAlpha4

   Plasmid

   #188050

   Purpose

   Expresses His8-FLI1_DBD_deltaAlpha4(276-361) with a TEV site for removal of the His8 tag.

   Depositor

   David Libich

   Article

   Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

   Insert

   FLI1 (FLI1 Human)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-275, 362-452

   Promoter

   T7

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG8Ha-ERG_DBD(306-429_F392A)

   Plasmid

   #188051

   Purpose

   Expresses His8-ERG_DBD(306-429_F392A) with a TEV site for removal of the His8 tag.

   Depositor

   David Libich

   Article

   Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

   Insert

   ERG (ERG Human)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-305, 430-479, mutated F392A

   Promoter

   T7

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG8Ha-FLI1_DBD(276-399_F362A)

   Plasmid

   #188047

   Purpose

   Expresses His8-FLI1_DBD(276-399_F362A) with a TEV site for removal of the His8 tag.

   Depositor

   David Libich

   Article

   Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

   Insert

   FLI1 (FLI1 Human)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-275, 400-452, mutated F362A

   Promoter

   T7

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOP795

   Plasmid

   #235700

   Purpose

   Protein expression His-taged HsNRBF2_FL in bacteria

   Depositor

   Roger Williams

   Article

   Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

   Insert

   NRBF2 (NRBF2 Human)
   

   Use

   Tags

   His6(TEV)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOP796

   Plasmid

   #235701

   Purpose

   Protein expression of His-tagged HsNRBF2 MIT domain in bacteria

   Depositor

   Roger Williams

   Article

   Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

   Insert

   NRBF2 MIT domain (NRBF2 Human)
   

   Use

   Tags

   His6(TEV)

   Expression

   Bacterial

   Mutation

   aa 1-89

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. A1-LCD allW D262V

   Plasmid

   #234616

   Purpose

   Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

   Depositor

   Tanja Mittag

   Article

   Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

   Insert

   hnRNPA1_LCD_allW D262V (HNRNPA1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   C-terminal domain (186-320) bearing familial ALS …

   Promoter

   T7

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. A1-LCD allW

   Plasmid

   #234609

   Purpose

   Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

   Depositor

   Tanja Mittag

   Article

   Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

   Insert

   hnRNPA1_LCD_allW (HNRNPA1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   C-terminal domain (186-320) with all aromatic ami…

   Promoter

   T7

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. A1-LCD allW D262N

   Plasmid

   #234615

   Purpose

   Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262N with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

   Depositor

   Tanja Mittag

   Article

   Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

   Insert

   hnRNPA1_LCD_allW D262N (HNRNPA1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   C-terminal domain (186-320) bearing familial ALS …

   Promoter

   T7

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hTln1-R12DD-SCLS

   Plasmid

   #191443

   Purpose

   expresses a variant of human talin1 domains R12-DD, with a deletion between K2375 and A2397

   Depositor

   Ben Goult

   Article

   Elefant et al JCI Insight. 2024 Dec 20;9(24):e173664.

   Insert

   Human Talin1 R12DD (TLN1 Human)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   deletion between residues K2375 and A2397

   Promoter

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG8Ha-EWS_RRM(359-447)

    Plasmid

    #188045

    Purpose

    Expresses His8-EWS_RRM(359-447) with a TEV site for removal of the His8 tag.

    Depositor

    David Libich

    Article

    Selig et al Biopolymers. 2023 May;114(5):e23536. doi

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-358, 446-656

    Promoter

    T7

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG8Ha-EWS_RRM-RGG(359-513)

    Plasmid

    #188046

    Purpose

    Expresses His8-EWS_RRM-RGG(359-513) with a TEV site for removal of the His8 tag.

    Depositor

    David Libich

    Article

    Selig et al Biopolymers. 2023 May;114(5):e23536. doi

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-358, 514-656

    Promoter

    T7

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBacHTA-NEDD4L_FL

    Plasmid

    #187722

    Purpose

    Express His-tagged NEDD4L FL in insect cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L FL (NEDD4L Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJExpress401-KDAC4 CD

    Plasmid

    #224251

    Purpose

    Expresses human KDAC4 (HDAC4) catalytic domain in E. coli

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC4 (HDAC4 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    Only includes residues 648-1083 (catalytic domain…

    Promoter

    T5

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pJExpress401-KDAC7 CD

    Plasmid

    #224341

    Purpose

    Expresses human KDAC7 (HDAC7) catalytic domain in E. coli

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC7 (HDAC7 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    Only includes residues 521-942 (catalytic domain).

    Promoter

    T5

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pJExpress401-KDAC8 H143A

    Plasmid

    #224342

    Purpose

    Expresses human KDAC8 (HDAC8) H143A in E. coli

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC8 H143A (HDAC8 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    Histidine 143 mutated to alanine

    Promoter

    T5

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pFastBacI-KDAC7 CD

    Plasmid

    #224345

    Purpose

    Expresses human KDAC7 (HDAC7) catalytic domain in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC7 (HDAC7 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Only includes residues 521-942 (catalytic domain).

    Promoter

    Polyhedrin

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pSDC803_IKZF1_mutant

    Plasmid

    #224113

    Purpose

    Insect cell expression vector for IKZF1 mutant for CRBN cryoEM pre-blotting

    Depositor

    Eric Fischer

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    IKZF1 (IKZF1 Human)
    

    Use

    Tags

    Flag-TEV

    Expression

    Insect

    Mutation

    Residues 140-196, Q146A and G151N

    Promoter

    polyhedrin

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-hMICU1EF

    Plasmid

    #199180

    Purpose

    This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.

    Depositor

    Ming-Feng Tsai

    Article

    Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    MICU1 (MICU1 Human)
    

    Use

    Tags

    His6 and MBP, Thrombin site, TEV site

    Expression

    Mutation

    D421A, E432K, F433W, F453W

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. K365-micro

    Plasmid

    #188457

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Zvonimir Dogic

    Article

    Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

    Insert

    Kinesin 1-365 (Khc Fly)
    

    Use

    Tags

    6xHis-MBP-TEV and SspB micro

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-365

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hTln1-R1R2-17b

    Plasmid

    #191427

    Purpose

    Expresses a variant of the human TLN1 R1R2 domains containing a 17AA insert into R2 (exon 17b) in bacteria

    Depositor

    Ben Goult

    Article

    Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901

    Insert

    Human Talin1 R1R2 domains (TLN1 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    contains 17AA insertion from inclusion of exon 17b

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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