We narrowed to 15,732 results for: LEA

1. pMKO.1-shSpc25

   Plasmid

   #160960

   Purpose

   Spc25 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Spc25 shRNA (Spc25 Mouse)
   

   Use

   RNAi and Retroviral

   Promoter

   U6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPS1-Venus

   Plasmid

   #133118

   Purpose

   Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pYPS1 driving a Venus.

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   pYPS1-YFP
   

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMK2-Venus

   Plasmid

   #133119

   Purpose

   Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pCMK2 driving a Venus.

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   pCMK2-YFP
   

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGYP7-Venus

   Plasmid

   #133120

   Purpose

   Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pGYP7 driving a Venus.

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   pGYP7-YFP
   

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15-His-∆NGlpG(Y205F)

   Plasmid

   #155132

   Purpose

   Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b

   Depositor

   Sinisa Urban

   Article

   Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

   Insert

   GlpG (glpG Escherichia coli)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Y205F, insert codes for GlpG residues 87-276

   Promoter

   T7

   Available Since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFloat.assemble(AB).LC_v1

   Plasmid

   #133083

   Purpose

   Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.

   Depositor

   Kacper Rogala, David Sabatini

   Article

   Rogala et al Science. 2019 Oct 25;366(6464):468-475.

   Type

   Empty backbone

   Use

   Destination vector

   Expression

   Bacterial

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFloat.assemble(AB).HC_v1

   Plasmid

   #133084

   Purpose

   Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.

   Depositor

   Kacper Rogala, David Sabatini

   Article

   Rogala et al Science. 2019 Oct 25;366(6464):468-475.

   Type

   Empty backbone

   Use

   Destination vector

   Expression

   Bacterial

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPOR1CB0015

   Plasmid

   #117551

   Purpose

   Level 1 Golden Gate Cassette: SaCas9 expression cassette for dicotyledonous plants

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   2x35S+5'UTR OMEGA (pICH51288) + SaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
   

   Expression

   Plant

   Promoter

   2x35S+5'UTR OMEGA (pICH51288)

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEPOR2KN0078

   Plasmid

   #117645

   Purpose

   Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
   

   Expression

   Plant

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET41a iGluSnFR E25R

    Plasmid

    #119831

    Purpose

    Production of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR E25R
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E25R

    Promoter

    T7

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNOC-CRISPR-compact

    Plasmid

    #99369

    Purpose

    Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS. No selection marker.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-Nlux

    Promoter

    Ribi promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. gamma2 UTR shRNA (shRNA4)

    Plasmid

    #120796

    Purpose

    shRNA targeting the 3'-untranslated region (UTR) of the gamma2 mRNA

    Depositor

    Angel de Blas

    Article

    Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

    Insert

    GABRG2 shRNA (Gabrg2 Rat)
    

    Use

    RNAi

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. gamma2 CR3m shRNA

    Plasmid

    #120795

    Purpose

    Negative control shRNA for plasmid 120794: shRNA targeting the coding region of the gamma2 mRNA

    Depositor

    Angel de Blas

    Article

    Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

    Insert

    GABRG2 shRNA (Gabrg2 Rat)
    

    Use

    RNAi

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. gamma2 UTR3m shRNA

    Plasmid

    #120797

    Purpose

    Negative control shRNA for plasmid 120796: shRNA targeting the 3'-untranslated region (UTR) of the gamma2 mRNA

    Depositor

    Angel de Blas

    Article

    Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

    Insert

    GABRG2 shRNA (Gabrg2 Rat)
    

    Use

    RNAi

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC17

    Plasmid

    #118875

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal tagRFP

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-tagRFP-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and tagRFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOCC16

    Plasmid

    #118870

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal EGFP

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-EGFP-stop-HindIII cassette
    

    Tags

    EGFP and HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOCC218

    Plasmid

    #118907

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS10, cleavable with 3C protease and N-terminal YBBR tag

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS10-3C-YBBR-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS10, cleavable with 3C protease, YBBR tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOCC18

    Plasmid

    #118868

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal tagRFP

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-tagRFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, tagRFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOCC171

    Plasmid

    #118888

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP tag

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-SNAP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease; SNAP tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOCC202

    Plasmid

    #118880

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mKate2, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    

    Tags

    mKate2, HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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