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We narrowed to 7,295 results for: ust

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Showing: 5381 - 5400 of 7295 results

  2. lentiCRISPR v2

    Plasmid
    #52961
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available Since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52961-plasmid-map-sequence-id-244694

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  3. pCRISPomyces-2

    Plasmid
    #61737
    Purpose
    Streptomyces expression of codon-optimized Cas9 and custom gRNA
    Depositor
    Huimin Zhao
    Article
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    gRNA cassette
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL) and rpsL(XC)-BbsI
    Available Since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61737-plasmid-map-sequence-id-190162

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  10. pYC1640

    Plasmid
    #158719
    Purpose
    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    Sth1 sgRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158719-plasmid-map-sequence-id-320840

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  11. pRPF215

    Plasmid
    #106377
    Purpose
    C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon
    Depositor
    Robert Fagan, Neil Fairweather
    Article
    Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1
    Insert
    Ptet-himar1-Ter(slpA)-transposon
    Use
    E. coli - c. difficile shuttle vector
    Mutation
    Codon optimised for C. difficile
    Promoter
    Ptet
    Available Since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    106377-plasmid-map-sequence-id-205135

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  13. CMV-O-GECO1

    Plasmid
    #46025
    Purpose
    Orange intensiometric genetically encoded Ca2+-indicators for optical imaging
    Depositor
    Robert Campbell
    Article
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Insert
    O-GECO1
    Expression
    Mammalian
    Mutation
    R-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E…
    Promoter
    CMV
    Available Since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    46025-plasmid-map-sequence-id-311519

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  17. pRBC-sfGFP-150TAG

    Plasmid
    #174076
    Purpose
    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,
    Depositor
    Ryan Mehl
    Article
    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15
    Insert
    Superfolder GFP
    Tags
    His-6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    T7
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    174076-plasmid-map-sequence-id-345697

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  18. CMV-R-GECO1.2

    Plasmid
    #45494
    Purpose
    Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2
    Depositor
    Robert Campbell
    Article
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Insert
    R-GECO1.2
    Expression
    Mammalian
    Mutation
    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…
    Promoter
    CMV
    Available Since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    45494-plasmid-map-sequence-id-123029

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  19. pYC2085

    Plasmid
    #158721
    Purpose
    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    Sth1 sgRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158721-plasmid-map-sequence-id-320843

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Showing: 5381 - 5400 of 7295 results