We narrowed to 6,018 results for: cki

1. TLNRD1_35-345_eGFP

   Plasmid

   #208881

   Purpose

   Express eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   TLNRD1 (TLNRD1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   amino acid sequence 35-345

   Promoter

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TLNRD1_5H_eGFP

   Plasmid

   #208861

   Purpose

   Express eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   TLNRD1 5 helix bundle (TLNRD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deleted amino acid 142-273

   Promoter

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLINE1ΔORF2-scFvFc

   Plasmid

   #206021

   Purpose

   The ORF2 sequence was removed from pLINE1-scFvFc

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF2 sequence
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLINE1ΔORF1-2-scFvFc

   Plasmid

   #206022

   Purpose

   The ORF1 and ORF2 sequences were removed from pLINE1-scFvFc

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNKX2-5-GFP-T2A

   Plasmid

   #203608

   Purpose

   Donor plasmid to create 5' knockin of NKX2-5 with GFP-T2A

   Depositor

   Matthew Birket

   Article

   Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

   Insert

   NKX2-5-GFP-T2A-NKX2-5 (NKX2-5 Human)
   

   Use

   Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJR017

   Plasmid

   #198835

   Purpose

   In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::TeTX-SL2-mKate::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHW491

   Plasmid

   #198808

   Purpose

   In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::Tetx cDNA::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3.1_B1ChR2_TS_mScarlet_ER

   Plasmid

   #195191

   Purpose

   Highly K+ selective channelrhodopsin

   Depositor

   Johannes Vierock, Peter Hegemann

   Article

   Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

   Insert

   Bilabrum sp. K+ selective channelrhodopsin 2
   

   Use

   Tags

   ER-export sequence, Kir2.1 trafficking motif, and…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-RAPH1Δ536-587

   Plasmid

   #194865

   Purpose

   Express eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   RAPH1 (RAPH1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   RAPH1 with amino acids 536-587 deleted

   Promoter

   CMV

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PL752-PTPRO-LA-p2a-tdTomato-hGHpA-PGK-puroR-PTPRO-RA

    Plasmid

    #191686

    Purpose

    For knocking p2a-tdTomato into PTPRO locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    PTPRO homology arms and p2a-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PL452-FGF8-LA-p2a-tdTomato-hGHpA-PGK-neoR-FGF8-RA

    Plasmid

    #191687

    Purpose

    For knocking p2a-tdTomato into FGF8 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    FGF8 homology arms and p2a-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PL452-PTPRO-LA-p2a-EGFP-hGHpA-PGK-neoR-PTPRO-RA

    Plasmid

    #191689

    Purpose

    For knocking p2a-EGFP into PTPRO locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    PTPRO homology arms and p2a-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIPKAH

    Plasmid

    #185869

    Purpose

    Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    GPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMiniT:myc-GluA2 TKIT donor

    Plasmid

    #169423

    Purpose

    Donor DNA for TKIT knockin of Myc to GluA2 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Myc (Myc Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-Miro1 (184-618)

    Plasmid

    #127626

    Purpose

    Human Miro1 lacking the first GTPase domain

    Depositor

    Josef Kittler

    Article

    Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

    Insert

    Miro1 (RHOT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    184-618 only

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His

    Plasmid

    #83457

    Purpose

    Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Amino-acids 4127-4303

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVH003

    Plasmid

    #80397

    Purpose

    Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experiments

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    Anti-scaffold - Venus YFP
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    Fused by GS linker to LZx domain and Venus has N-…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRFP shRNA-resistant TrkB F616A -S478A

    Plasmid

    #72718

    Purpose

    Mammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant and lacking TIAM1-binding site

    Depositor

    Freddy Jeanneteau

    Article

    Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

    Insert

    TrkB
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    shRNA-resistant TrkB (T300C, T301A, C302G, C306A,…

    Promoter

    CMV

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pRevLCav2deltaC

    Plasmid

    #60809

    Purpose

    to make truncation of yeast Rev3

    Depositor

    Tahir Tahirov, Youri Pavlov

    Article

    Siebler et al DNA Repair (Amst). 2014 May 9. pii: S156

    Insert

    REV3 (REV3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    rev3ΔC encodes for Rev3 lacking the C-terminus (a…

    Promoter

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-PIM2mut3

    Plasmid

    #53742

    Purpose

    promoter region of PIM2 lacking the 2 forkhead binding sites

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    PIM2 (PIM2 Human)
    

    Use

    Luciferase; Reporter vector

    Tags

    Expression

    Mutation

    deletion of 2 forkhead binding sites (bp# 395-497…

    Promoter

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only