We narrowed to 9,203 results for: sacs

1. CD4-C-BFP HDRT Source (pTR 176)

   Plasmid

   #112020

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   CD4-BFP HDRT (CD4 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CD4-C-mCherry HDRT Source (pTR 175)

   Plasmid

   #112019

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   CD4-mCherry HDRT (CD4 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYO395

   Plasmid

   #235734

   Purpose

   Expression of human VPS34 complex I in mammalian cells

   Depositor

   Roger Williams

   Article

   Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

   Inserts

   ATG14 (ATG14 Human)
   EGFP
   BECN1 (BECN1 Human)
   PIK3C3 (PIK3C3 Human)
   PIK3R4 (PIK3R4 Human)
   

   Use

   Tags

   ZZ-3xTEV

   Expression

   Mammalian

   Mutation

   N256S

   Promoter

   CMV

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213028

   Purpose

   Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   E43S D145N double mutant EN- endonuclease catalyt…

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213027

   Purpose

   Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   D702Y (RT- reverse transcriptase catalytic dead)

   Promoter

   CMV

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213029

   Purpose

   Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FoxO6deltaCt

   Plasmid

   #124486

   Purpose

   Expresses dominant negative form of mouse FoxO6 with deleted C-term transactivation domain. T26A/S184A mutations increase nuclear localization of FoxO6.

   Depositor

   Anne Brunet

   Article

   Salih et al Genes Dev. 2012 Dec 15;26(24):2780-801.

   Insert

   FoxO6 (Foxo1, Foxo4, Foxo3, Foxo6 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T26A, S184A, transactivation domain deleted from …

   Promoter

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC19_18GZG

   Plasmid

   #117228

   Purpose

   shble flanked by an endogenous GAPDH promoter and terminator system

   Depositor

   Jackie Collier

   Article

   Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

   Insert

   shble
   

   Use

   Thraustochytrid expression

   Tags

   Expression

   Mutation

   Promoter

   GAPDH

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. DinoIII-neo

   Plasmid

   #155026

   Purpose

   Dinoflagelate backbone for amino 3'-glycosyl phosphotransferase (neo) gene

   Depositor

   Senjie Lin

   Article

   Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

   Insert

   amino 3'-glycosyl phosphotransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DinoIII-pat

    Plasmid

    #155028

    Purpose

    Dinoflagelate backbone for Phosphinothricin N-acetyltransferase, pat, gene

    Depositor

    Senjie Lin

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Insert

    Phosphinothricin N-acetyltransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. DinoIII-bsr

    Plasmid

    #155027

    Purpose

    Dinoflagelate backbone for blasticidin S-resistance gene (bsr)

    Depositor

    Senjie Lin

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Insert

    Blasticidin-S deaminase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC:FCP:ShBle:FCP:EGFP

    Plasmid

    #85987

    Purpose

    Expresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.

    Depositor

    Thomas Mock

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Inserts

    ShBle
    EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    FCP

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJH122

    Plasmid

    #68377

    Purpose

    pJH110 variant Hygromycin

    Depositor

    Trevor Charles

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJRT8

    Plasmid

    #68385

    Purpose

    PHA metabolic engineering

    Depositor

    Trevor Charles

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJRT12

    Plasmid

    #68388

    Purpose

    PHA metabolic engineering

    Depositor

    Trevor Charles

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJRT9

    Plasmid

    #68386

    Purpose

    PHA metabolic engineering

    Depositor

    Trevor Charles

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJH123

    Plasmid

    #68379

    Purpose

    pJH122 variant Gateway destination

    Depositor

    Trevor Charles

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJRT16

    Plasmid

    #68390

    Purpose

    PHA metabolic engineering, similar to pJRT9, with R388 oriT

    Depositor

    Trevor Charles

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pJRT15

    Plasmid

    #68389

    Purpose

    PHA metabolic engineering, similar to pJRT8 with R388 oriT

    Depositor

    Trevor Charles

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF1alpha-rTetR-bGHpA

    Plasmid

    #172285

    Purpose

    Constitutive expression of reverse tetracycline-controlled transactivator

    Depositor

    Patrik Verstreken

    Insert

    rTetR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    EF1alpha

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. tTA IRES GFP

    Plasmid

    #18783

    Depositor

    Scott Lowe

    Insert

    tTA
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

22. pJH104

    Plasmid

    #68384

    Purpose

    Integration vector for creating Sinorhizobium meliloti Landing Pad Strain

    Depositor

    Trevor Charles

    Insert

    attP-aadA-uidA-attP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRevTRE-FLMKL1

    Plasmid

    #27162

    Depositor

    Ron Prywes

    Insert

    MKL1 (MRTFA Human)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

24. pTIN-ura4

    Plasmid

    #161808

    Purpose

    A ura-selectable lncRNA-regulated gene expression system with rapid induction kinetics in the fission yeast Schizosaccharomyces pombe

    Depositor

    Stewart Shuman

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pho1

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pILFPQ13A7

    Plasmid

    #223837

    Purpose

    Expressing mScarletI3 under the control of the TEF1 promoter in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Insert

    pTEF1>mScarletI3>
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pILFPQ13B7

    Plasmid

    #223838

    Purpose

    Expressing StayGold under the control of the TEF1 promtoer in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Insert

    pTEF1>StayGold>
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pILFPZ1A9

    Plasmid

    #223835

    Purpose

    Expressing a cytosolic pH sensor (mTagBFP2-mNeonGreen) in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Insert

    pTEF1>mTagBFP>mNeonGreen>
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pILFPmTagBFP2FrFAST

    Plasmid

    #223839

    Purpose

    Expressing mTagBFP2-FrFAST under the control of the TEF1 promtoer in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Insert

    pTEF1>mTagBFP2>FrFAST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pILFPQ13BE5

    Plasmid

    #223836

    Purpose

    Expressing a cytosolic cAMP sensor (mScarletI3-ApacH-StayGold2) in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Insert

    pTEF1>mScarletI3>ApacH>StayGold2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR_SFFV_tTA_IRES_tagBFP

    Plasmid

    #231953

    Purpose

    Lentiviral vector for constitutive expression of the tetracycline transactivator tTA and of the blue fluorescent protein tagBFP, separated by an internal ribosome entry site (SFFV promoter)

    Depositor

    Leonardo Morsut

    Insert

    tTA_IRES_tagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge