We narrowed to 18,759 results for: nls
-
Plasmid#163463Purposelentiviral vector expressing Cas9 and an sgRNA targeting METAP1DepositorInsertsgRNA 2 targeting METAP1 (METAP1 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgMPC2_7
Plasmid#163457Purposelentiviral vector expressing Cas9 and an sgRNA targeting MPC2DepositorInsertsgRNA 7 targeting GPT2 (MPC2 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiCRISPR-v1-sgMPC2_9
Plasmid#163458Purposelentiviral vector expressing Cas9 and an sgRNA targeting MPC2DepositorInsertsgRNA 9 targeting GPT2 (MPC2 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUPD2 PhiC31 integrase (GB1496)
Plasmid#160578PurposeStreptomyces phage PhiC31 integrase, complete CDSDepositorInsertPhiC31
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)
Plasmid#160616PurposeTU for the constitutive expression of Streptomyces phage PhiC31 integrase.DepositorInsertPhiC31
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2: Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos-Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1679)
Plasmid#160619PurposeModule for estradiol-inducible expression of the PhiC31 integrase gene and dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.DepositorInsertERLexABDGal4AD / PhiC31 / GRLacIBDGal4AD / RDF
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3_alpha2 35:PhyB:VP16 (GB2745)
Plasmid#160630PurposeTU for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switchDepositorInsertPhyBVP16
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD391 VPR-ZF37
Plasmid#138839PurposeConstitutive expression of VPR-ZF37 under the CMV promoter.DepositorInsertVPR-ZF37
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330 L-FKBP-Cas9
Plasmid#162728PurposeMammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330 LC-FKBP-Cas9
Plasmid#162724PurposeMammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgUpf1
Plasmid#161810Purposeguide RNA for Upf1DepositorInsertsgRNA targeting mouse Upf1 (Upf1 Mouse, Synthetic)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
(517) SB KRAB-SadCas9 U6-gStop
Plasmid#163023PurposeSleeping Beauty TET-On expression of CRISPRi with gSTOP gRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMammalian codon-optimized SadCas9
UseTransposonTagsKRAB and myc NLSExpressionMammalianPromoterTet-OnAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p33Fn
Plasmid#158155PurposeExpression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRExpressionPlantMutationRice codon optimizedAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD708 DsRed-Express2-ZF21
Plasmid#138795PurposeConstitutive expression of DsRed-Express2-ZF21 under the CMV promoter.DepositorInsertDsRed-Express2-ZF21
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD159 dsRedExpress2-ZFi43
Plasmid#138799PurposeConstitutive expression of dsRedExpress2-ZFi43 under the CMV promoter.DepositorInsertdsRedExpress2-ZFi43
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD539 VP16-ZF173
Plasmid#138773PurposeConstitutive expression of VP16-ZF173 under the CMV promoter.DepositorInsertVP16-ZF173
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD526 VP16-ZF13
Plasmid#138755PurposeConstitutive expression of VP16-ZF13 under the CMV promoter.DepositorInsertVP16-ZF13
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD528 VP16-ZF21
Plasmid#138757PurposeConstitutive expression of VP16-ZF21 under the CMV promoter.DepositorInsertVP16-ZF21
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_RBMX-eA3A-UdgX-NG-nCas9
Plasmid#163551PurposeMammalian CG-to-GC base editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRBMX-eA3A-UdgX-NG-nCas9
UseCRISPRExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…Available sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-EE-UdgX-NG-nCas9-RBMX
Plasmid#163571PurposeMammalian CG-to-GC base editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUdgX-EE-UdgX-NG-nCas9-RBMX
UseCRISPRExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…Available sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2300 - [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
Plasmid#159869PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIE053 ZF43x6-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR
Plasmid#138734PurposeIntegration Vector, assembled with pHIE045 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613DepositorInsertZF43x6-Compact
UseSynthetic BiologyExpressionMammalianAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti AID-BEmax
Plasmid#157950PurposeLentiviral expression of AID-BEmaxDepositorInsertLenti AID-BEmax
UseCRISPR and LentiviralExpressionMammalianAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-p150-PIP2Δ
Plasmid#160994PurposeExpress mouse p150 protein with PIP2 deletion mutationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCHAF1A (Chaf1a Mouse)
TagsGFPExpressionMammalianMutationPIP2 motif deletionAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
907L
Plasmid#160237PurposeFlightN-ttgR-VP16-Sv40-attBDepositorInsertsTtgR repressor
Flightin promoter
TagsVP16ExpressionInsectAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3
Plasmid#159912PurposeMutagenesis of Kcnd3DepositorInsertKcnd3 (Kcnd3 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgKcnq3
Plasmid#159911PurposeMutagenesis of Kcnq3DepositorInsertKcnq3 (Kcnq3 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgNts
Plasmid#159909PurposeMutagenesis of NtsDepositorInsertNts (Nts Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TetR-Rad18UBD
Plasmid#119010PurposeMammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTetR
ExpressionMammalianPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6Rosa-CAG-Cas9
Plasmid#118998PurposeMammalian expression of Rosa26 sgRNA, Cas9 and BFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
ExpressionMammalianPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-986B
Plasmid#124999PurposeExpresses dCas9-VPR driven by the Ubiquitin-63E promoterDepositorInsertdCas9-VPR
UsePiggybackExpressionInsectPromoterUbiquitinAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-2_PolyU(1)_Tornado-Corn
Plasmid#159487PurposeTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-2_PolyU(1)
ExpressionMammalianPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
ABEMax nSaCas9
Plasmid#135364PurposeExpresion of SaCas9 nickase (D10A) with ABEMax at the N terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABEMax nSaCas9
UseCRISPRExpressionMammalianMutation(V82G)Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9-AIDx
Plasmid#135343PurposeExpression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnSpyCas9-hAIDx
UseCRISPRExpressionMammalianMutationWTAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre sgAmotl2 v3
Plasmid#158602Purposelenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2DepositorInsertAmotl2 sgRNA
ExpressionMammalianPromoterU6Available sinceSept. 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
nuc-yHS1-M7A,H102A
Plasmid#159168PurposeNuclear labile heme reporterDepositorInsertnuc-yHS1-M7A,H102A
TagsSV40 NLSExpressionYeastMutationMutated both Met 7 and His 102 of the cyt b562 mo…PromoterGPDAvailable sinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPK-351
Plasmid#157921PurposepcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBDDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsPIF3-MTAD
IRES
PhyB(1-621)-SV40NLS-Gal4DBD
UseSynthetic Biology; OptogeneticsTagsHA tag and SV40NLSExpressionMammalianMutationpif3 1-523PromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dox nontargeting shRNA GFP
Plasmid#155290PurposeLentivirus for doxycycline inducible expression of nontargeting shRNA, GFP marker, based on Addgene 11652DepositorInsertNontargeting shRNA
ExpressionMammalianAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
PABE-7-NG
Plasmid#138142PurposeTargeted A to G in rice protoplastsDepositorInsertwtTadA-TadA7.10-nCas9-NG-3XNLS
ExpressionPlantAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AAVS1-TALEN-L
Plasmid#159299PurposeTALEN (left) targeting the AAVS1 locus with obligate heterodimer FokIDepositorInsertTALEN targeting the AAVS1
UseTALENExpressionMammalianPromoterCMV promoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only