We narrowed to 9,184 results for: Pol;

1. pCAG-mCherry-FKBP-GEF(TIAM1)

   Plasmid

   #85156

   Purpose

   acute activation of Rac through rapamycin-induced translocation to membrane-localized FRB

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Fusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)
   

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-p58

   Plasmid

   #166942

   Purpose

   mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFP

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

   Insert

   p58 (Lman1 Rat)
   

   Tags

   GFP and chicken lysozyme signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmRFP-N1 human cofilin S3A

   Plasmid

   #50857

   Purpose

   expresses constituvely active cofilin fused to mRFP in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3A (CFL1 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   S3A constitutively active

   Promoter

   CMV

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

4. AAV-CAG-FLInChR-mVenus

   Plasmid

   #119298

   Purpose

   FLinChR is the fusion signal sequence and transmembrane (TM) domain of Neurexin 1B-delta to ChR2E123T/T159C for inversion ChR2 to an optogenetic inhibitor

   Depositor

   Alla Karpova

   Article

   Brown et al Cell. 2018 Oct 16. pii: S0092-8674(18)31

   Insert

   Nx1BTM-FCS-ChR2ET/TC-mVenus
   

   Use

   AAV

   Tags

   mVenus

   Promoter

   CAG

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMKIIa-eGFP-Cre

   Plasmid

   #120219

   Purpose

   Expresses cre under the control of CamKIIa promotor

   Depositor

   Marie Carlén

   Article

   Ahrlund-Richter et al Nat Neurosci. 2019 Apr;22(4):657-668. do

   Insert

   cre
   

   Use

   AAV and Cre/Lox

   Tags

   EGFP

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-KDM4A

   Plasmid

   #188233

   Purpose

   Express human KDM4A in mammalian cells

   Depositor

   Young Tang

   Article

   Su et al Int J Mol Sci. 2021 Sep 28;22(19). pii:

   Insert

   Lysine-specific demethylase 4A (KDM4A Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA12-pHR-SFFV-BFP-B23

   Plasmid

   #199443

   Purpose

   Expression of BFP-B23 in mammalian cells

   Depositor

   Baohui Chen

   Article

   Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

   Insert

   B23 (NPM1 Human)
   

   Use

   Lentiviral

   Tags

   TagBFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSR646

   Plasmid

   #65215

   Purpose

   Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells

   Depositor

   Robert Kotin

   Article

   Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

   Inserts

   modified AAV-2 rep78
   modified AAV-6 cap
   

   Use

   AAV

   Expression

   Insect

   Promoter

   P10 and polyhedrin

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRE-VEGFA EF1a-rtTA3

   Plasmid

   #195881

   Purpose

   Lentiviral construct of doxycycline inducible VEGFA

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Insert

   VEGFA (Vegfa Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE3GS

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSicoR-mCh-empty

    Plasmid

    #21907

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

    Depositor

    Miguel Ramalho-Santos

    Article

    Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPINS-USP21 (USP, aa 196-565)

    Plasmid

    #61585

    Purpose

    Expresses human USP21 (USP domain) in E. coli.

    Depositor

    David Komander

    Article

    Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

    Insert

    USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
    

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Deleted aa 1-195.

    Promoter

    T7

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. cASPG1-mRFP

    Plasmid

    #181962

    Purpose

    ASPG1 protein tagged with mRFP, under control of TBA2 promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    ASPG1 (ASPG1 Mustard Weed)
    

    Tags

    mRFP

    Expression

    Plant

    Promoter

    TBA2 promoter

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV BARK D110A p2A mCherry

    Plasmid

    #117690

    Purpose

    CMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

    Depositor

    Baljit Khakh

    Article

    Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

    Insert

    BARKrgs D110A peptide
    

    Tags

    HA / FLAG

    Expression

    Mammalian

    Mutation

    D110A

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRE-STOP EF1a-rtTA3

    Plasmid

    #195882

    Purpose

    control construct of TRE-VEGFA EF1a-rtTA3

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    VEGFA (Vegfa Synthetic, Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3GS

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTSlb-wt

    Plasmid

    #60728

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 human cofilin S3E

    Plasmid

    #50855

    Purpose

    expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 S3E (CFL1 Human)
    

    Expression

    Mammalian

    Mutation

    S3E psuedo-phosporylated, non-activatable

    Promoter

    CMV

    Available Since

    Feb. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. C1A-C1A-EYFP

    Plasmid

    #61155

    Purpose

    Diacylglycerol (DAG) reporter

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C1A from PKCG amino acids 26-89

    Promoter

    CMV

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. W118-1_hTGM2-V5

    Plasmid

    #180413

    Purpose

    lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM2 (TGM2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSR660

    Plasmid

    #65216

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-8 cap
    

    Use

    AAV

    Expression

    Insect

    Promoter

    P10 and polyhedrin

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX6P-2-human RNF146 full length

    Plasmid

    #132610

    Purpose

    expresses human RNF146 full length in E. coli

    Depositor

    Wenqing Xu

    Article

    DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

    Insert

    RNF146 (RNF146 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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