We narrowed to 32,111 results for: eng

1. pMSIP124: pDonor(CyCAST)_0.5kb-cargo

   Plasmid

   #200848

   Purpose

   CyCAST donor harboring non-functional cargo.

   Depositor

   Feng Zhang

   Article

   Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

   Insert

   CyCAST
   

   Expression

   Bacterial

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSIP267: pHelper(CyCAST)_PSP1_ΔTniQ

   Plasmid

   #200842

   Purpose

   Inducible expression of CyCAST proteins (except TniQ) with crRNA targeting CyPSP1.

   Depositor

   Feng Zhang

   Article

   Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

   Insert

   CyCAST
   

   Expression

   Bacterial

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSIP269: pHelper(CyCAST)_PSP1_ΔTnsD

   Plasmid

   #200844

   Purpose

   Inducible expression of CyCAST proteins (except TnsD) with crRNA targeting CyPSP1.

   Depositor

   Feng Zhang

   Article

   Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

   Insert

   CyCAST
   

   Expression

   Bacterial

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMSIP116: pHelper(CyCAST)_PSP1

   Plasmid

   #200840

   Purpose

   Inducible expression of CyCAST proteins with crRNA targeting CyPSP1.

   Depositor

   Feng Zhang

   Article

   Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

   Insert

   CyCAST
   

   Expression

   Bacterial

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMSIP122: pHelper(CyCAST)_Shuffled-spacer

   Plasmid

   #200841

   Purpose

   Inducible expression of CyCAST proteins with control crRNA for CyPSP1.

   Depositor

   Feng Zhang

   Article

   Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

   Insert

   CyCAST
   

   Expression

   Bacterial

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMZ103-rbs1-m3

   Plasmid

   #204465

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127S mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Mutation

   LasR L125W-A127S mutation

   Promoter

   In an operon and PLas

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMZ103-rbs1-m4

   Plasmid

   #204466

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Mutation

   LasR L125W-A127T mutation

   Promoter

   In an operon and PLas

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMZ103-rbs1-m5

   Plasmid

   #204467

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125F-A127G-S129N mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Mutation

   LasR L125F-A127G-S129N mutation

   Promoter

   In an operon and PLas

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMZ103-rbs1-m6

   Plasmid

   #204468

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-S129N-L130V mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Mutation

   LasR L125W-S129N-L130V mutation

   Promoter

   In an operon and PLas

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMZ103-rbs1-m7

    Plasmid

    #204469

    Purpose

    This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T-L128M mutation.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

    Inserts

    quorum sensing regulator LasR (lasR )
    eYFP
    

    Use

    Synthetic Biology

    Mutation

    LasR L125W-A127T-L128M mutation

    Promoter

    In an operon and PLas

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMZ103-rbs1-m8

    Plasmid

    #204470

    Purpose

    This is a biosensor with decreased specificity to 3OC12-HSL and improved specificity to 3OHC14-HSL, containing LasR L128F-S129W mutation.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

    Inserts

    quorum sensing regulator LasR (lasR )
    eYFP
    

    Use

    Synthetic Biology

    Mutation

    LasR L128F-S129W mutation

    Promoter

    In an operon and PLas

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNDGG007

    Plasmid

    #231311

    Purpose

    Vector part for BEVA; BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
    

    Expression

    Bacterial

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNDGG001

    Plasmid

    #231307

    Purpose

    Vector part for BEVA; BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRExT2A-NT-BTy

    Plasmid

    #232206

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRExT2A-CT-PTy

    Plasmid

    #232203

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-PAC-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRExT2A-CT-BTy

    Plasmid

    #232209

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-BSD-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRExT2A-NT-PTy

    Plasmid

    #232202

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-Cas9-FCPF

    Plasmid

    #220132

    Purpose

    Expresses Cas9-FCPF in E.coli

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    Cas9-FCPF (cas9 S. pyogenes)
    

    Tags

    His and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. dCas9-FCPF

    Plasmid

    #220131

    Purpose

    Expresses dCas9-FCPF in mammalian cells

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    dCas9-FCPF (cas9 S. pyogenes, Synthetic)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-dCas9-FCPF

    Plasmid

    #220133

    Purpose

    Expresses dCas9-FCPF in E.coli

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    dCas9-FCPF (cas9 S. pyogenes, Synthetic)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    FCPF sequence inserted on C-terminal

    Promoter

    T7

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge