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We narrowed to 70,836 results for: LAS

Showing: 8161 - 8190 of 70836 results

  1. pNSU299_YBL055C

    Plasmid
    #166077
    Purpose
    Plasmid for constituive spCas9 and tet-inducible YBL055C targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    YBL055C (near PTC3) (YBL055C Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166077-plasmid-map-sequence-id-329132

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  2. pNSU299_YNR071C

    Plasmid
    #166081
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of YNR071C for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of YNR071C (YNR071C Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166081-plasmid-map-sequence-id-329131

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  3. pNSU299_SLA1

    Plasmid
    #166075
    Purpose
    Plasmid for constituive spCas9 and tet-inducible SLA1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    SLA1 (SLA1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166075-plasmid-map-sequence-id-329130

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  4. pNSU299_PPE1

    Plasmid
    #166083
    Purpose
    Plasmid for constitutive spCas9 and tet-inducible PPE1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    PPE1 (PPE1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166083-plasmid-map-sequence-id-329122

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  5. pNSU299_PDR12

    Plasmid
    #166093
    Purpose
    Plasmid for constituive spCas9 and tet-inducible PDR12 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    PDR12 (PDR12 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166093-plasmid-map-sequence-id-329121

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  6. pNSU299_TDH2

    Plasmid
    #166082
    Purpose
    Plasmid for constituive spCas9 and tet-inducible TDH2 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    TDH2 (TDH2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166082-plasmid-map-sequence-id-329117

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  7. pNSU299_LYS9

    Plasmid
    #166085
    Purpose
    Plasmid for constituive spCas9 and tet-inducible LYS9 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    LYS9 (LYS9 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166085-plasmid-map-sequence-id-329116

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  8. pUPD2 nickase D10A Cas9 (GB1691)

    Plasmid
    #160588
    Purpose
    Nickase D10A Cas9 for Nt fusion.
    Depositor
    Diego Orzaez
    Insert
    nickase D10A Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160588-plasmid-map-sequence-id-322078

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  9. J-CDAN1A-5

    Plasmid
    #166522
    Purpose
    scFv of a human scaffold targeting Codanin-1. Antigen coverage aa 840-1202 of 1227
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166522-plasmid-map-sequence-id-328268

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  10. Z-OPTN-10

    Plasmid
    #166562
    Purpose
    scFv of a human scaffold targeting Optineurin. Antigen coverage aa 1-510 of 577
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    166562-plasmid-map-sequence-id-327812

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  11. PB CAG-sfGFP-GSAx9-iCre-ERT2

    Plasmid
    #137857
    Purpose
    CAG-sfGFP-GSAx9-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    sfGFP-GSAx9-iCre-ERT2
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    137857-plasmid-map-sequence-id-316181

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  12. FUW-hTie1-ECD-FLAG

    Plasmid
    #119235
    Purpose
    Plasmid encodes FLAG-tagged extracellular domain of human Tie1 gene cloned in lentiviral vector.
    Depositor
    Kari Alitalo
    Insert
    TIE1-ECD-FLAG (TIE1 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Contains extracellular domain (ECD) only
    Promoter
    P hUbC
    Available since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    119235-plasmid-map-sequence-id-231733

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  13. GLT-1b delta 4

    Plasmid
    #98707
    Purpose
    mammalian expression of Glt1b delta 4 mutant
    Depositor
    Paul Rosenberg
    Insert
    Glt1b (Slc1a2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    missing last 4 amino acids at the C-terminus. I52…
    Promoter
    Available since
    Sept. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    98707-plasmid-map-sequence-id-193958

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  14. STK10

    Plasmid
    #74659
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    STK10 (STK10 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. pJK692

    Plasmid
    #71643
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His17>Trp/Ser38>Thr mutant (TdPurE2-H17W,S38T)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-17 to tryptophan, serine-38 to …
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    71643-plasmid-map-sequence-id-131664

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  16. pJK653

    Plasmid
    #71630
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Thr/Phe79>Trp mutant (TdPurE2-S38T,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to threonine, phenylalanine-79 …
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71630-plasmid-map-sequence-id-131649

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  17. pJK654

    Plasmid
    #71631
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Ala/Phe79>Trp mutant (TdPurE2-S38A,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to alanine, phenylalanine-79 to…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71631-plasmid-map-sequence-id-131651

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  18. pJK655

    Plasmid
    #71632
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp/Phe79>Trp mutant (TdPurE2-S38D,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to aspartate, phenylalanine-79 …
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71632-plasmid-map-sequence-id-131652

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  19. pJK656

    Plasmid
    #71633
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Val/Phe79>Trp mutant (TdPurE2-S38V,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to valine, phenylalanine-79 to …
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71633-plasmid-map-sequence-id-131653

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  20. pJK659

    Plasmid
    #71634
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Cys/Phe79>Trp mutant (TdPurE2-S38C,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to cysteine, phenylalanine-79 t…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71634-plasmid-map-sequence-id-131654

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  21. pJK660

    Plasmid
    #71635
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met/Phe79>Trp mutant (TdPurE2-K41M,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes lysine-41 to methionine, phenylalanine-79…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71635-plasmid-map-sequence-id-131655

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  22. pJK661

    Plasmid
    #71636
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Arg/Phe79>Trp mutant (TdPurE2-K41R,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes lysine-41 to arginine, phenylalanine-79 t…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71636-plasmid-map-sequence-id-131656

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  23. pJK662

    Plasmid
    #71637
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp/Cys87>Ser mutant (TdPurE2-F79W,C87S)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes phenylalanine-79 to tryptophan, cysteine-…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71637-plasmid-map-sequence-id-131657

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  24. pJK689

    Plasmid
    #71642
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asn/Phe79>Trp mutant (TdPurE2-S38N,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to asparagine, phenylalanine-79…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71642-plasmid-map-sequence-id-131663

    Enlarge

  25. pJK697

    Plasmid
    #71646
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met15>Trp/Ser38>Thr mutant (TdPurE2-M15W,S38T)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes methionine-15 to tryptophan, serine-38 to…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71646-plasmid-map-sequence-id-131667

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  26. pJK657

    Plasmid
    #71647
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His40>Ala/Phe79>Trp mutant (TdPurE2-H40A,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-40 to alanine, phenylalanine-79…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71647-plasmid-map-sequence-id-131642

    Enlarge

  27. pJK658

    Plasmid
    #71648
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His40>Asn/Phe79>Trp mutant (TdPurE2-H40N,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-40 to asparagine, phenylalanine…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71648-plasmid-map-sequence-id-131644

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  28. pKPY737

    Plasmid
    #72673
    Purpose
    Encodes Thr412Gly-CePheRS Alpha Subunit (Gateway Entry Clone)
    Depositor
    David Tirrell
    Insert
    Thr412Gly-CePheRS Alpha Subunit::C. elegans CEOP5428 Intercistronic Region::HA-GFP [From pPD95.75 (+118 to +1000)] in pDONR221
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Thr412Gly in CePheRS Alpha Subunit
    Promoter
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    72673-plasmid-map-sequence-id-133998

    Enlarge

  29. 4QOX

    Plasmid
    #61959
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-CDPK4
    Use
    Baculovirus expression
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Mutation
    See comments
    Promoter
    Polyhedrin
    Available since
    May 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. 4GM2

    Plasmid
    #60132
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PF14_0348:PF14_0348_dml-X03:C228094
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 8161 - 8190 of 70836 results