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pcDNA-h-mmPtch1-B-L234C-HA

Plasmid

#120905

Purpose

Expresses Ptch1-B (L234C)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, L…

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Sesn-C mSesn2 (delAB)

Plasmid

#111799

Purpose

Mammalian Expression of mSesn2

Depositor

Jun Hee Lee

Article

Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

Insert

mSesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

AB domain truncation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Sesn-B+C mSesn2 (delA)

Plasmid

#111798

Purpose

Mammalian Expression of mSesn2

Depositor

Jun Hee Lee

Article

Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

Insert

mSesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

A domain truncation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Sesn-A mSesn2 (delBC)

Plasmid

#111797

Purpose

Mammalian Expression of mSesn2

Depositor

Jun Hee Lee

Article

Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

Insert

mSesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

BC domain truncation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2B-3/4

Plasmid

#91359

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2B-3/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2B-3/4 (SH3PXD2B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2B-2/4

Plasmid

#91316

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2B-2/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2B-2/4 (SH3PXD2B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2B-1/4

Plasmid

#91317

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2B-1/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2B-1/4 (SH3PXD2B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2B-4/4

Plasmid

#91257

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2B-4/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2B-4/4 (SH3PXD2B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Sema3g.a(S)-Fc-His

Plasmid

#72152

Purpose

Expresses the Sema3G, isoform a protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.a (Sema3g Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3g.z(S)-Fc-His

Plasmid

#72153

Purpose

Expresses the Sema3G, isoform z protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.z (Sema3g Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3g.z(S)-AP-His

Plasmid

#72027

Purpose

Expresses the Sema3G, isoform z protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.z (Sema3g Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6d.2-AP-His

Plasmid

#72043

Purpose

Expresses the extracellular region of the Sema6D, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.2 (Sema6d Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6d.2-Fc-His

Plasmid

#72169

Purpose

Expresses the extracellular region of the Sema6D, isoform 2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.2 (Sema6d Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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KHBD00090

Plasmid

#34246

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9610 (Poxm Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
Neurl1-Flag pBacPAK8-3

Plasmid

#17322

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
NHR1-Flag pBacPAK8-3

Plasmid

#17323

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pET28a(cd96)

Plasmid

#177901

Purpose

Bacterial expression of his10-tagged human cd96 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

CD96 (aa 557-585 only) (CD96 Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pYL192 (TRV1)

Plasmid

#148968

Purpose

Tobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)

Depositor

Savithramma Dinesh-Kumar

Article

Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

Insert

TRV RNA1

Use

Plant vector with trv rna1; this vector used with…

Mutation

P697S mutation in putative replicase. S239G and S…

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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TurboID-EV

Plasmid

#215085

Purpose

Expresses TurboID in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

TurboID (birA )

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_Ef1a:PE2-P2A-GFP

Plasmid

#184445

Purpose

Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

Depositor

Bobby Koeleman

Article

Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and Ef1A

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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