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PSD-95 scFv [K28/43]

Plasmid

#182066

Purpose

Mammalian Expression of PSD-95 scFv. Derived from hybridoma K28/43.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting PSD-95 (Homo sapiens) (DLG4 Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-DNTGFBR2-HA

Plasmid

#130888

Purpose

Expresses dominant negative mutant human TGFBR2

Depositor

Gian-Paolo Dotto

Article

Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

Insert

TGFBR2 (TGFBR2 Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

Dominant Negative receptor lacking cytosolic cata…

Promoter

CMV

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PSD-95 scFv [K28/74]

Plasmid

#190490

Purpose

Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/74.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pADC10-SETXhel

Plasmid

#193056

Purpose

Baculovirus expression of human Senataxin helicase domain for protein production

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

Senataxin (SETX Human)

Use

Tags

Flag

Expression

Insect and Mammalian

Mutation

codon optimized for insect cell expression

Promoter

hybrid p10 (insect) and CMV (human) expression

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Homer1L scFv [L113/27]

Plasmid

#190502

Purpose

Mammalian Expression of Homer1L scFV. Derived from hybridoma L113/27.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Homer1L (Mus musculus) recombinant scFV (Homer1 Mouse)

Use

Tags

Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-GIGYF2

Plasmid

#141189

Purpose

Expresses GFP-GIGYF2 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

GIGYF2 (GIGYF2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-LAR/PTPRF [N165/38R]

Plasmid

#177504

Purpose

Mammalian Expression Plasmid of anti-LAR/PTPRF (Human). Derived from hybridoma N165/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-LAR/PTPRF (Homo sapiens) recombinant mouse monoclonal antibody (PTPRF Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_P2A_Puro

Plasmid

#211364

Purpose

Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

Depositor

Jacques Neefjes

Article

Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-PTPR [N165/43R]

Plasmid

#188219

Purpose

Mammalian Expression Plasmid of anti-Pan-PTPR. Derived from hybridoma N165/43

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-PTPR recombinant mouse monoclonal antibody (PTPRF Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

Plasmid

#213969

Purpose

AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT A41AA C55A CRFR1 95TAG 263TAA-HA

Plasmid

#154781

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAG 263TAA, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

CRFR1 (Crhr1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

95TAG 263TAA in CRFR1-HA, hybrid PylT with A41AA …

Promoter

EF1

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherryC1 VAP-B KD/MD

Plasmid

#226410

Purpose

Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to mCherry in mammalian cells

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

VAP-B (VAPB Human)

Use

Tags

HA, T7-Xpress tag, and mCherry

Expression

Mammalian

Mutation

K87D/M89D mutant, unable to bind FFAT motifs

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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Cav1.2 Ca2+ channel scFv [N263/31]

Plasmid

#182083

Purpose

Mammalian Expression of Cav1.2 Ca2+ channel scFv. Derived from hybridoma N263/31.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Cav1.2 Ca2+ channel (Rattus norvegicus) (Cacna1c Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-mCherry-FLAG-cGAS E225A/D227A

Plasmid

#127657

Purpose

Over-express catalytically inactive mCherry-FLAG-cGAS E225A/D227A

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

Flag and mCherry

Expression

Mutation

E225A/D227A

Promoter

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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Kir2.1 K+ channel scFv [N112/16]

Plasmid

#206769

Purpose

Mammalian Expression of Kir2.1 K+ channel scFV. Derived from hybridoma N112/16 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir2.1 K+ channel (Mus musculus) recombinant scFV (Kcnj2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Kir2.1 K+ channel scFv [N112B/29]

Plasmid

#206770

Purpose

Mammalian Expression of Kir2.1 K+ channel scFV. Derived from hybridoma N112B/29 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir2.1 K+ channel (Mus musculus) recombinant scFV (Kcnj2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Kir2.1 K+ channel scFv [N112B/14]

Plasmid

#199423

Purpose

Mammalian expression of Kir2.1 K+ channel scFv. Derived from hybridoma N112B/14.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingKir2.1 K+ channel (Rat) (Kcnj2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-STAT-CA

Plasmid

#195576

Purpose

Expresses STAT3 constitutively active mutant

Depositor

Kevin Park

Article

Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

Insert

Signal transducer and activator of transcription 3 (Stat3 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

changed Alanine 662 to Cysteine and Asparagine 66…

Promoter

CMV enhancer and promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-RNF2-ngRNA+41_EF1a-puroR

Plasmid

#207358

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

RNF2 +41 ngRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-OCRL/INPP5b [N166A/26]

Plasmid

#190301

Purpose

Mammalian Expression Plasmid of anti-OCRL/INPP5b (Human). Derived from hybridoma N166A/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-OCRL/INPP5b (Homo sapiens) recombinant Mouse monoclonal antibody (OCRL Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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