We narrowed to 14,052 results for: CRISPR-Cas9

Showing: 5461 - 5480 of 14052 results

Results per page:

Sort results by:

pYPQ151

Plasmid

#69302

Purpose

Gateway entry vector with pcoCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9D10A (Plant codon-optimized)

Use

CRISPR

Tags

2XFLAG

Mutation

D10A mutation; NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC1 Halo-Actb KI

Plasmid

#183426

Purpose

CreON knock-in for Halo-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo donor

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ172

Plasmid

#98409

Purpose

Donor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Inserts

upstream homologous region to delete lys9 in genome by recombination
downstream homologous region to delete lys9 in genome by recombination

Use

CRISPR and Synthetic Biology

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ132D

Plasmid

#69288

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ131D

Plasmid

#69287

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ155

Plasmid

#69306

Purpose

Gateway entry vector with AteCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9D10A (Arabidopsis codon optimized)

Use

CRISPR

Mutation

D10A mutation; NLS fusion

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC2 Tubb3-GFP KI

Plasmid

#183432

Purpose

CreOFF knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC1 Gria1-HA KI

Plasmid

#183427

Purpose

CreON knock-in for GluA1-HA (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and HA donor

Use

CRISPR

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC2 Dlg4-Halo KI

Plasmid

#183449

Purpose

CreOFF knock-in for PSD95-Halo (amino acid position: R721)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo donor

Use

CRISPR

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

Plasmid

#196710

Purpose

Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-R4R3-3xmEGFP-C

Plasmid

#113751

Purpose

Gateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP-mEGFP-mEGFP

Use

CRISPR and Gateway Cloning

Tags

3xmEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC4 Actb-mRuby3 KI

Plasmid

#183437

Purpose

FlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and mRuby3 donor

Use

CRISPR

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC5 Tubb3-GFP KI

Plasmid

#183440

Purpose

FlpOFF knock-in for beta3-tubulin GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ138A

Plasmid

#69280

Purpose

Golden Gate entry vector; 8th gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ137A

Plasmid

#69279

Purpose

Golden Gate entry vector;7th gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-R4R3-3xmRuby-N

Plasmid

#113754

Purpose

Gateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2-mRuby2

Use

CRISPR and Gateway Cloning

Tags

3xmRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pIK198

Plasmid

#65629

Purpose

"flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE Tubb3-4xGFP KI

Plasmid

#183445

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and 4xGFP donor

Use

CRISPR

Expression

Mammalian

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-R4R3-mEGFP-N

Plasmid

#113746

Purpose

Gateway multi-site entry clone for second position mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP

Use

CRISPR and Gateway Cloning

Tags

mEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-R4R3-mEGFP-C

Plasmid

#113749

Purpose

Gateway multi-site entry clone for second position mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP

Use

CRISPR and Gateway Cloning

Tags

mEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge