We narrowed to 18,904 results for: Tec

1. pCfB6637

   Plasmid

   #106164

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6681 into Yarrowia lipolytica chromosomal location IntE_3, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b+R2(L171C)

   Plasmid

   #108239

   Purpose

   Expresses PdR-PCNA2(L171C) fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PdR-PCNA2(L171C) fusion protein
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   PCNA2,L171C

   Promoter

   T7 promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15T+1X

   Plasmid

   #108188

   Purpose

   PCNA1-PdX fusion protein

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PCNA1-PdX fusion protein
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   C73S/C85S (PdX)

   Promoter

   T7 promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLENTICRISPR sgNFS1

   Plasmid

   #102979

   Purpose

   Cutting NFS1 genomic locus

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   sgNFS1 (NFS1 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-NMBR-eLOV-TEVcs-FLAG-GAL4-V5

   Plasmid

   #104843

   Purpose

   b2AR-SPARK TF component

   Depositor

   Alice Ting

   Article

   Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

   Insert

   NMBR-eLOV-TEVcs-FLAG-GAL4-V5
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. YIp128-P30-His-pol30(K127/164R)

   Plasmid

   #99545

   Purpose

   Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; Leu2 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   POL30 (POL30 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   K127R, K164R

   Promoter

   POL30

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNA

   Plasmid

   #87572

   Purpose

   PV4 gRNA entry vector for CRISPR decoder

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBW1943_ hU6-PV3-FRT-loxP-BbsI-ENTRY-gRNA

   Plasmid

   #89056

   Purpose

   PV3 gRNA entry vector for CRISPR decoder

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3_zfh1_CP-candidate_luc+

   Plasmid

   #86391

   Purpose

   Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer
   

   Use

   Luciferase

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pILGFPB6

    Plasmid

    #83557

    Purpose

    Used to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL10 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. const #7-RFP

    Plasmid

    #81081

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Promoter

    Constitutive Promoter - BBa_J23109

    Available Since

    Aug. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pconst #6-RFP

    Plasmid

    #81080

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Promoter

    Constitutive Promoter - BBa_J23117

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. shCDK4(1)

    Plasmid

    #73550

    Purpose

    shRNA(1) against CDK4

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

14. shCDK4(2)

    Plasmid

    #73551

    Purpose

    shRNA(2) against CDK4

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCellFree_G03 TCEB3B

    Plasmid

    #67117

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TCEB3B (ELOA2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCellFree_G03 ZFYVE9

    Plasmid

    #67134

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ZFYVE9 (ZFYVE9 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Mutation

    F1359I

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pATN (pKD-G12)

    Plasmid

    #62691

    Purpose

    Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

    Insert

    amiR-eGFP419/amiR-eGFP123/tdTomato
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pATL (pKD-G10)

    Plasmid

    #62689

    Purpose

    Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

    Insert

    amiR-eGFP419/amiR-eGFP123
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pARA (pKD-G1)

    Plasmid

    #62680

    Purpose

    Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

    Insert

    tdTomato/amiR-eGFP123
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pARB (pKD-G2)

    Plasmid

    #62681

    Purpose

    Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

    Insert

    tdTomato/amiR-eGFP419
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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