We narrowed to 13,571 results for: GAN

1. pCAGGS puro HA-hUSP27X Y381H

   Plasmid

   #225723

   Purpose

   Transfection of USP27X (Y381H) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X Y381H (USP27X Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y381H

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAGGS puro HA-hUSP27X F313V

   Plasmid

   #225722

   Purpose

   Transfection of USP27X (F313V) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X F313V (USP27X Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   F313V

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS puro HA-hUSP27X K181E

   Plasmid

   #225721

   Purpose

   Transfection of USP27X (K181E) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X K181E (USP27X Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K181E

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAGGS puro HA-hUSP27X Y144C

   Plasmid

   #225720

   Purpose

   Transfection of USP27X (Y144C) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X Y144C (USP27X Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y144C

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAGGS puro HA-hUSP27X G76S

   Plasmid

   #225719

   Purpose

   Transfection of USP27X (G76S) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X G76S (USP27X Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G76S

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX6P1 GST-hUSP27X S404N

   Plasmid

   #225717

   Purpose

   Bacterial expression of USP27X (S404N) with GST tag

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X S404N (USP27X Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   S404N

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX6P1 GST-hUSP27X Y381H

   Plasmid

   #225716

   Purpose

   Bacterial expression of USP27X (Y381H) with GST tag

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X Y381H (USP27X Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Y381H

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX6P1 GST-hUSP27X F313V

   Plasmid

   #225715

   Purpose

   Bacterial expression of USP27X (F313V) with GST tag

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X F313V (USP27X Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F313V

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX6P1 GST-hUSP27X Y144C

   Plasmid

   #225713

   Purpose

   Bacterial expression of USP27X (Y144C) with GST tag

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X Y144C (USP27X Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Y144C

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX6P1 GST-hUSP27X G76S

    Plasmid

    #225712

    Purpose

    Bacterial expression of USP27X (G76S) with GST tag

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X G76S (USP27X Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G76S

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX6P1 GST-hUSP27X C87A

    Plasmid

    #225711

    Purpose

    Bacterial expression of USP27X (C87A) with GST tag

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X C87A (USP27X Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C87A, inactivates deubiquitylase

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCI AscI MR1 R9H res

    Plasmid

    #214753

    Purpose

    expresses human MR1 R9H resistant to CRISPR editing with a specific sgRNA in mammalian cells

    Depositor

    David Lewinsohn

    Article

    Kulicke et al Commun Biol. 2024 Feb 24;7(1):228. doi:

    Insert

    MHC class I-related protein 1 (MR1 Human)
    

    Tags

    IRES eGFP

    Expression

    Mammalian

    Mutation

    silent mutations to prevent editing by CRISPR/Cas…

    Promoter

    CMV

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i3-14-GFP10

    Plasmid

    #180349

    Purpose

    mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Promoter

    bidirectional TRE

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTRIP TRE BI_SEPT9_i3-14-GFP11_SEPT9_i3-14-GFP10

    Plasmid

    #180352

    Purpose

    mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT9_i3 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN9_v3 (SEPTIN9 Human)
    SEPTIN9_v3 (SEPTIN9 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Promoter

    bidirectional TRE

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTRIP TRE BI_GFP10-14-SEPT7 Gmut2_GFP11-14-SEPT7 Gmut2

    Plasmid

    #180353

    Purpose

    mammalian co-expression of human SEPT7 Gmut2 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN7 (SEPTIN7 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Mutation

    SEPT7 H279D

    Promoter

    bidirectional TRE

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTRIP TRE BI_SEPT9_i1-14-GFP10_GFP11-14-SEPT7 Gmut2

    Plasmid

    #180359

    Purpose

    mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN 9 (SEPTIN9 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Mutation

    SEPT7 H279D

    Promoter

    bidirectional TRE

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRIP TRE BI_SEPT9_i3-14-GFP10_GFP11-14-SEPT7 Gmut2

    Plasmid

    #180361

    Purpose

    mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Mutation

    SEPT7 H279D

    Promoter

    bidirectional TRE

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTRIP TRE BI_GFP11-14-SEPT7_GFP10-14-SEPT7

    Plasmid

    #180347

    Purpose

    mammalian co-expression of human SEPT7 fused to GFP10 and of human SEPT7 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN7 (SEPTIN7 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Promoter

    bidirectional TRE

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP_SEPT6

    Plasmid

    #180312

    Purpose

    bacterial co-expression of human SEPT2 NCmut fused to monomeric superfolder GFP and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    SEPT2 F20D, V27D

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT2iU6Apowriter2N(#355)

    Plasmid

    #184106

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transient transgenesis + in vitro trans…

    Tags

    internal tag: 5myc

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi+SP6

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-T7-NLS(SV40)-anCas(SCA)-BPNLS(SV40)-P2A-EGFP (LTH1378)

    Plasmid

    #185484

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(SCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-T7-NLS(SV40)-anCas(PCA)-BPNLS(SV40)-P2A-EGFP (LTH1441)

    Plasmid

    #185486

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(PCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-T7-NLS(SV40)-anCas(FCA)-BPNLS(SV40)-P2A-EGFP (LTH1380)

    Plasmid

    #185487

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(FCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

    Plasmid

    #185489

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

    Plasmid

    #185492

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. zebrafish similar to gpr151 (or gpcr-2037)_L (OZ535)

    Plasmid

    #27212

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) (LOC565170 Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

27. zebrafish similar to gpr151 (or gpcr-2037)_R (OZ536)

    Plasmid

    #27213

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) (LOC565170 Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

28. pScaffold-H1 donor

    Plasmid

    #118152

    Purpose

    PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSpCas9(BB)-T2A-HygR

    Plasmid

    #118153

    Purpose

    SpCas9 expression vector

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    hSpCas9-T2A-HygR
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CMV-PDGFRb-HA

    Plasmid

    #204350

    Purpose

    Expresses wild-type PDGFRb gene. Used for DNA delivery using Adeno-associated Virus.

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Use

    AAV

    Tags

    HA

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV2-sgTrp53-sgAtrx-EFS-Cre

    Plasmid

    #189977

    Purpose

    Expresses Cre cDNA and sgRNAs targeting murine Trp53 and Atrx

    Depositor

    William Kaelin

    Article

    Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

    Insert

    sgTrp53, sgAtrx, Cre recombinase
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pF1-Hs Cdk1/cyclin B1/Cks1

    Plasmid

    #177329

    Purpose

    Co-expression of the human active Cdk1/cyclin B1/Cks1 kinase complex

    Depositor

    Andreas Boland

    Article

    Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

    Inserts

    Cyclin-dependent kinase 1 (CDK1 Human)
    G2/mitotic-specific cyclin-B1 (CCNB1 Human)
    Cyclin-dependent kinases regulatory subunit 1 (CKS1B Human)
    

    Tags

    2 x Strep-tag and 8 x His

    Expression

    Insect

    Promoter

    p10 and polh

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. KRD22_HBA1(HBA1reg-HBB-longHAs)

    Plasmid

    #232403

    Purpose

    AAV production plasmid for HBA1 long HAs vector from Figs. 3-6 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank full HBA1-2A-YFP cassette. HAs are ~900bp

    Depositor

    Kyle Cromer, Matthew Porteus

    Article

    Cromer et al Nat Med. 2021 Apr;27(4):677-687. doi: 10

    Inserts

    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Human Hemoglobin Subunit Beta (HBB Human)
    

    Expression

    Mammalian

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMB.PRC2

    Plasmid

    #234579

    Purpose

    Expression of MBP-EZH2, MBP-SUZ12, MBP-EED and MBP-RBBP4

    Depositor

    Chen Davidovich

    Article

    Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

    Insert

    EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)
    

    Expression

    Insect

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMSCV-MerCreMer-hygro

    Plasmid

    #188982

    Purpose

    Retroviral expression of tamoxifen-inducible Cre recombinase

    Depositor

    William Kaelin

    Article

    Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

    Insert

    Tamoxifen-inducible Cre recombinase
    

    Use

    Cre/Lox and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCAGGS-PDGFRb559-562del-IRES2-EGFP

    Plasmid

    #204355

    Purpose

    Expresses a mutant PDGFRb gene (559-562 deletion).

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Mutation

    559-562 deletion

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTRIP TRE BI_SEPT2 NCmut-14-GFP11_SEPT2 NCmut-14-GFP10

    Plasmid

    #180357

    Purpose

    mammalian co-expression of human SEPT2 NCmut fused to GFP10 and of human SEPT2 NCmut fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN 2 (SEPTIN2 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Mutation

    SEPT2 F20D, V27D

    Promoter

    bidirectional TRE

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTRIP TRE BI_SEPT9_i1 NCmut-14-GFP11_SEPT9_i1 NCmut-14-GFP10

    Plasmid

    #180358

    Purpose

    mammalian co-expression of human SEPT9_i1 NCmut fused to GFP10 and of human SEPT9_i1 NCmut fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN9_v1 (SEPTIN9 Human)
    SEPTIN9_v1 (SEPTIN9 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Mutation

    SEPT9_i1 I281D, M288D

    Promoter

    bidirectional TRE

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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39. pAAV-CMV-PDGFRb559-562del-HA

    Plasmid

    #204351

    Purpose

    Expresses a mutant PDGFRb gene (559-562 deletion). Used for DNA delivery using Adeno-associated Virus.

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Use

    AAV

    Tags

    HA

    Mutation

    559-562 deletion

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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40. lentiSAMv2 ZFAND3-guide4

    Plasmid

    #125516

    Purpose

    CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a core and U6

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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