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pBABE-NLS-HA-TurboID-PCNA

Plasmid

#215074

Purpose

Expresses NLS-HA-TurboID-PCNA in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PCNA (PCNA Human)

Use

Retroviral

Tags

NLS-HA-TurboID

Expression

Mammalian

Promoter

LTR

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-W531C

Plasmid

#116210

Purpose

Lentiviral expression of BRAF W531C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

W531C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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FFSS-Cyclin D1-NeoR

Plasmid

#215087

Purpose

Expresses FFSS-Cyclin D1 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Tags

FFSS (FLAG-FLAG-STREP-STREP)

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_MKRN1_BRAF

Plasmid

#205857

Purpose

Express mEGFP-tagged fusion protein, MKRN1_BRAF from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

MKRN1_BRAF (MKRN1, BRAF Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Desmoplakin I affinity mutant (F40-based tension sensor)

Plasmid

#119190

Purpose

The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.

Depositor

Alex Dunn

Article

Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

Insert

human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)

Use

Transposon

Tags

mTFP1-F40-mEYFP

Expression

Mammalian

Mutation

inserted F40-based tension sensor module after aa…

Promoter

TCE

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_BMPR1B_WT

Plasmid

#82195

Purpose

Gateway Donor vector containing BMPR1B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BMPR1B (BMPR1B Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-D594A

Plasmid

#116135

Purpose

Lentiviral expression of BRAF D594A

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

D594A

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RAF1

Plasmid

#116785

Purpose

Lentiviral expression of RAF1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RAF1 (RAF1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-L597R

Plasmid

#116178

Purpose

Lentiviral expression of BRAF L597R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

L597R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-L597S

Plasmid

#116179

Purpose

Lentiviral expression of BRAF L597S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

L597S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-FFSS-Cyclin D1 (T286A)

Plasmid

#215147

Purpose

Expresses FFSS-Cyclin D1 (T286A) in mammalian cells from an inducible lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

FFSS (FLAG-FLAG-STREP-STREP)

Expression

Mammalian

Mutation

With T286A mutation.

Promoter

TRE3GS

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G464V

Plasmid

#116148

Purpose

Lentiviral expression of BRAF G464V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

G464V

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-L597V

Plasmid

#116180

Purpose

Lentiviral expression of BRAF L597V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

L597V

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G469E

Plasmid

#116154

Purpose

Lentiviral expression of BRAF G469E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

G469E

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G464E

Plasmid

#116146

Purpose

Lentiviral expression of BRAF G464E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

G464E

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G466V

Plasmid

#116152

Purpose

Lentiviral expression of BRAF G466V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

G466V

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G469V

Plasmid

#116157

Purpose

Lentiviral expression of BRAF G469V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

G469V

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-APTX

Plasmid

#215128

Purpose

Expresses mCherry-APTX in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

APTX (APTX Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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p21-mCerulean-NeoR

Plasmid

#215080

Purpose

Expresses p21-mCerulean in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_CSF2RA_CRLF2

Plasmid

#205798

Purpose

Express mEGFP-tagged fusion protein, CSF2RA_CRLF2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

CSF2RA_CRLF2 (CSF2RA, CRLF2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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