We narrowed to 12,177 results for: LEA
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Plasmid#119835PurposeProduction of recombinant GluBP600n high affinity protein in E. coliDepositorInsertGluBP600n
TagsHisExpressionBacterialPromoterT7Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BtuCD
Plasmid#92208PurposeExpression of BtuCD in E. coliDepositorTags10x Histidine Tag and Enterokinase Cleavage SiteExpressionBacterialPromoterT7 PromoterAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNB774
Plasmid#60114PurposeGreen beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)DepositorInsertGrLuc
UseUnspecifiedTagsClpXP degron and Nuclear localization signal (NLS…Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
PP-HSP90 (3Q5J)
Plasmid#28281DepositorInsertPP-HSP90
TagsHis tag with TEV cleavageExpressionBacterialMutationN-terminal domainAvailable sinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1177
Plasmid#29091PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXcX-G4BS-3.6TPH-EGFP
Plasmid#32572DepositorInsertEGFP
UseAdenoviralExpressionMammalianPromoter3.6TPHAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
zgc:153086_R (OZ512)
Plasmid#27187DepositorInsertZinc finger array targeting zgc:153086
UseZebrafish targetingAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA)
Plasmid#192442PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.DepositorInsertmCherry-TSC2(S939A,T1439A) (TSC2 Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERKsub(TA)DD-Venus-NES
Plasmid#84631PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertERKsub(TA)DD-Venus-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationMutated target Thr residue to Ala.PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKCsub(T/A)-YPet-NES
Plasmid#138208PurposeEncodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertPKCsub(T/A)-YPet-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceSept. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Drosha270-1374delNLS2
Plasmid#62532PurposeN-terminal aa1-269 and nuclear localization signal 2 of Drosha are deletedDepositorInserttruncated human Drosha mutant (DROSHA Human)
TagsGFPExpressionMammalianMutationdeleted amino acids 1-269 and nuclear localizatio…Available sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4-R115A/R119A
Plasmid#80696PurposeExpresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4-R115A/R119A
UseAAVExpressionMammalianMutationArg115 and Arg119 both mutated to AlaPromoterCBAAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N1-cofilin
Plasmid#186750PurposeExpresses wildtype mCherry-tagged human cofilin in mammalian cellsDepositorAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-37
Plasmid#214346PurposeProtein overexpression in bacteriaDepositorAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-DNPH1
Plasmid#203224PurposeFor protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2
Plasmid#196526PurposeEntry vector for Gateway with Eps8L2DepositorAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV U6-sgRNA UbC-GFP
Plasmid#106948PurposesgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP markerDepositorInsertsgRNA BsmBI/Esp3I cloning site
UseCRISPR and LentiviralExpressionMammalianAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
EC2_1_dCas9_sgRNA
Plasmid#163710PurposeYeast low copy plasmid with dCas9 and sgRNA expression cassetteDepositorInsertdCas9
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA528-AMOT(1-300)-sortase
Plasmid#166706Purposeexpresses AMOT(1-300) with C-terminal sortase tag in bacterial cellsDepositorInsertAMOT aa 1-300
TagsHis-SUMO and Sortase tagExpressionBacterialPromoterT7 promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTM
Plasmid#162783PurposeProtein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are codedDepositorTypeEmpty backboneTags10xHis, c-myc, and hTPA leaderExpressionMammalianPromoterCHO Elongation factor 1AAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only