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iRFP670-PIP5K1A

Plasmid

#202749

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iRFP670:PIP5K1A (PIP5K1A Human)

Expression

Mammalian

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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CLP1

Plasmid

#155462

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CLP1 (CLP1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-MOSPD2 RD/LD

Plasmid

#186476

Purpose

Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Publication

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-HsBIN1_tv8

Plasmid

#176012

Purpose

Mammalian expression of Human EGFP-Bin1 (transcriptional variant 8, muscle specific). Parton lab clone HBH

Depositor

Rob Parton

Publication

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bin1

Tags

EGFP

Expression

Mammalian

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp5

Plasmid

#163589

Purpose

Expresses BMP-5 in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bmp5 (Bmp5 Mouse)

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDH-EF1a-extGluc-NGFR

Plasmid

#104832

Purpose

extGluc luciferase for invivo Imaging, NGFR for flourescent detection or separation

Depositor

Irmela Jeremias

Publication

Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

Inserts

extGLuc
NGFR

Use

Lentiviral and Luciferase

Expression

Mammalian

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pFA-LEU2-I-SceI

Plasmid

#67595

Purpose

I-SceI expression regulated by tetracycline inducible system in S. pombe

Depositor

Kurt Runge

Publication

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

nmt terminator
I-SceI
TetR

Use

Gene targeting in yeast

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184S

Plasmid

#106189

Purpose

Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184S

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

Promoter

CAG

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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mEos2-EB3-7

Plasmid

#57372

Purpose

Localization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

EB3 (MAPRE3 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pRB20

Plasmid

#52559

Purpose

serves as PCR-template to generate HR donors for C-terminal YFP-tag

Depositor

Klaus Foerstemann

Publication

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

eYFP and Blasticigin resistance cassette

Use

Blunt cloning kit

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa34_1384

Plasmid

#49693

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF34_1384

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal L95E

Plasmid

#47344

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

L95E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pDONR223-CKS2

Plasmid

#23841

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CKS2 (CKS2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK11

Plasmid

#23816

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK11 (NEK11 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
PCXGW

Plasmid

#48257

Purpose

Split-GFP assay in plants

Depositor

Wolf Frommer

Publication

Kim et al Plant Cell. 2009 Apr;21(4):1305-23. doi:

Type

Empty backbone

Use

Binary gateway vector

Tags

6xHIS and CFP aa155-238

Promoter

35S

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-20nm-EBFP2

Plasmid

#231185

Purpose

Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 20 nm

Depositor

Dmitry Lim

Publication

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

20nm-EBFP2

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Spacer Switch Plasmid

Plasmid

#215346

Purpose

Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

Depositor

Hank Seifert

Publication

Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

Insert

OpaD spacer; Chloramphenicol Resistance Cassette

Use

CRISPR

Expression

Bacterial

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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ZCCHC6

Plasmid

#156140

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ZCCHC6 (TUT7 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin

Plasmid

#195092

Purpose

Gene Overexpression of Human SHARPIN

Depositor

Jukka Westermarck

Publication

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Available since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pFUdioFRTeYFPW

Plasmid

#133998

Purpose

expresses eYFP upon coexpression with the recombinase flippase (Flp)

Depositor

Elly Nedivi

Publication

Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

Insert

eYFP

Use

Lentiviral; Flp/frt

Expression

Mammalian

Mutation

inverted and flanked by two incompatible FRT site…

Promoter

UbC

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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