We narrowed to 8,320 results for: Set

1. PB-TAC-OSKM

   Plasmid

   #80481

   Purpose

   piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

   Depositor

   Knut Woltjen

   Article

   Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

   Insert

   OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

   Plasmid

   #132389

   Purpose

   Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   human TauWT 2N4R-EGFP (MAPT Synthetic, Human)
   

   Use

   CRISPR and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   TREtight

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

   Plasmid

   #132393

   Purpose

   Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   human TauP301L 2N4R-EGFP (MAPT Synthetic, Human)
   

   Use

   CRISPR and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Proline 301 to Leucine (P301L)

   Promoter

   TREtight

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

   Plasmid

   #171802

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-mgp100-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ai148 (TIT2L-GC6f-ICL-tTA2) targeting vector

   Plasmid

   #114428

   Purpose

   Target a Cre-dependent GCaMP6f expression and a tTA2 cassette into the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   GCaMP6f
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   TRE2, CAG

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAU003 - pTRE3G-Cterm_Nterm_switch_CTCF-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

   Plasmid

   #156430

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Cterm_Nterm_switched

   Promoter

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. G protein alpha-o-YFP

   Plasmid

   #55776

   Purpose

   This G protein alpha-o construct contains internal insertions of YFP and the EE epitope

   Depositor

   Catherine Berlot

   Article

   Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

   Insert

   G protein alpha-o internally tagged with EYFP and EE epitope (Gnao1 Rat)
   

   Use

   Tags

   EE epitope was introduced internally with D167E a…

   Expression

   Mammalian

   Mutation

   A BglII site was removed from the 5' untrans…

   Promoter

   CMV

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ai133 (TITL-ssAPEX2tm) targeting vector

   Plasmid

   #114432

   Purpose

   Target a Cre-dependent electron microscopy tag ssAPEX2tm cassette into the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   ssAPEX2tm
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   TREtight

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ai140 (TIT2L-GFP-ICL-tTA2) targeting vector

   Plasmid

   #114427

   Purpose

   Target a Cre-dependent GFP and a tTA2 expression cassette into the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   GFP, tTA2
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK, CAG

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid

    #156434

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    All zinc fingers mutated

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMCRW1

    Plasmid

    #122462

    Purpose

    Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells

    Depositor

    Richard C. Willson

    Article

    Kourentzi et al Sci Rep. 2020 Mar 19;10(1):5078. doi: 10

    Insert

    NPM-ALK fusion protein (ALK, NPM1 Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac promoter

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Spike Display_Part 2 DO

    Plasmid

    #172722

    Purpose

    Encodes a sfGFP dropout expression cassette in place of Part 2 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MADR pDonor TagBFP2-3XFlag (cyto) WPRE

    Plasmid

    #129421

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-3Flag
    

    Use

    Mosaic analysis for dual recombinase-mediated cas…

    Tags

    3X Flag epitope tag

    Expression

    Mutation

    Promoter

    none (3X polyA to mitigate episomal expression)

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

    Plasmid

    #171801

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-hgp100-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAav-MDM2-PQS2-3xHA

    Plasmid

    #84886

    Purpose

    rAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassette

    Depositor

    Sven Eyckerman

    Article

    Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

    Inserts

    MDM2 genomic sequence HR1 (MDM2 Human)
    MDM2 genomic insert HR2 (MDM2 Human)
    

    Use

    AAV

    Tags

    PQS2 3xHA

    Expression

    Mutation

    Promoter

    no

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid

    #156429

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    DELTA1-265

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. retro-gfpIkkb-puro vector

    Plasmid

    #58251

    Purpose

    Retroviral expression vector encoding bicistronic EGFP-IKKbeta and Puromycin cassette

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Insert

    Ikkβ (Ikbkb Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-TAC-MKOS

    Plasmid

    #80484

    Purpose

    piggyBac transposon for dox-inducible expression of the MKOS polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    MKOS (Myc, Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPaint-mScarlet-F-Ova-PuroR [M1G]

    Plasmid

    #171813

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-Ova-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122

    Plasmid

    #223186

    Purpose

    Plasmid containing the Zfr/YAP5SA/3xMIR122 cassette. Translation of YAP5SA is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo.

    Depositor

    Yuxuan Guo

    Article

    Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

    Inserts

    YAP5SA (Yap1 Mouse)
    mir122 (Mir122 Mouse)
    

    Use

    AAV

    Tags

    HA and engineered P2A

    Expression

    Mutation

    S61A, S109A, S127A, S164A, S381A

    Promoter

    TNNT2

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. P532_POU4F2-p2A-tdTomato-p2A-Thy1.2

    Plasmid

    #239102

    Purpose

    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of POU4F2 (aka BRN3B). This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

    Depositor

    Karl Wahlin

    Article

    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

    Insert

    POU4F2 (POU4F2 Human)
    

    Use

    Donor plasmid for integration into the human geno…

    Tags

    p2A-tdTomato-p2A-Thy1.2

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous POU4F2

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. P530_SIX6-p2A-h2b-EGFP

    Plasmid

    #239101

    Purpose

    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of SIX6. This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

    Depositor

    Karl Wahlin

    Article

    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

    Insert

    SIX6 (SIX6 Human)
    

    Use

    Donor plasmid

    Tags

    p2A-h2b-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous SIX6 promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MKC86_HBA1(tEPOR-2A-YFP)

    Plasmid

    #232405

    Purpose

    AAV production plasmid for tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at HBA1 locus using HBA1-sg4 gRNA. HBA1 UTRs flank full tEPOR-2A-YFP cassette. Homology arms mediate full HBA1 replacement.

    Depositor

    Matthew Porteus, Kyle Cromer

    Article

    Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552

    Inserts

    Truncated Erythropoietin Receptor (EPOR Human)
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. KRD9_HBA1(HBA1reg-HBB)

    Plasmid

    #232402

    Purpose

    AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank HBA1 cassette. HAs are ~400bp each.

    Depositor

    Matthew Porteus, Kyle Cromer

    Article

    Cromer et al Nat Med. 2021 Apr;27(4):677-687. doi: 10

    Insert

    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. KRD2_HBA1(HBA1reg-UbC-GFP)

    Plasmid

    #232401

    Purpose

    AAV production plasmid for HBA1 WGR vector from Fig. 1 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBA1 UTRs flank UbC-GFP cassette; GFP is followed by BGH polyA. HAs are ~400bp each.

    Depositor

    Matthew Porteus, Kyle Cromer

    Article

    Cromer et al Nat Med. 2021 Apr;27(4):677-687. doi: 10

    Insert

    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)

    Plasmid

    #176141

    Purpose

    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    XRCC1-T2A-PolB (XRCC1, POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP and MYC

    Expression

    Mammalian

    Mutation

    Myc-tagged PolB with mutation in Asp256 to Ala, a…

    Promoter

    EF1A

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Spike Display_Part 1 DO

    Plasmid

    #172721

    Purpose

    Encodes a sfGFP dropout expression cassette in place of Part 1 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCRISPaint-mNeon-F-gB-PuroR [M1G]

    Plasmid

    #171803

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-gB-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Spike Display_Part-5 DO

    Plasmid

    #172725

    Purpose

    Encodes a sfGFP dropout expression cassette in place of Part 5 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Spike Display_Part 3 DO

    Plasmid

    #172723

    Purpose

    Encodes a sfGFP dropout expression cassette in place of Part 3 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge