We narrowed to 11,681 results for: cel.2

1. dora[A]_rescue_construct

   Plasmid

   #190639

   Purpose

   Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Truncated to contain amino acids 1-945

   Promoter

   Actin-5c

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PX458-Lck

   Plasmid

   #159430

   Purpose

   CRISPR knockout of Lck

   Depositor

   Arthur Salomon

   Article

   Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

   Insert

   sgRNA targeting Lck exon 2 (LCK Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ELK3_pet28a

   Plasmid

   #131651

   Purpose

   Bacterial expression of ETS gene ELK3

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ELK3 (ELK3 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FLT3-D835Y-MSCV-IRES-GFP

   Plasmid

   #184780

   Purpose

   Expresses FLT3 D835Y activating tyrosine kinase domain mutant in mammalian cells

   Depositor

   Timothy Ley

   Insert

   FLT3 (FLT3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Activating tyrosine kinase domain mutation (D835Y)

   Promoter

   MSCV

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pc DNA FRT TO- APP695

   Plasmid

   #114193

   Purpose

   Creation of a stable cell line expressing APP mutation (AD in vitro study)

   Depositor

   Aleksandra Radenovic

   Insert

   Amyloid-Precursor-Protein (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wild type splice variant 695: 695AA (2085bp, lack…

   Promoter

   CMV

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FUW-TetO-Dppa2

   Plasmid

   #40799

   Depositor

   Rudolf Jaenisch

   Article

   Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

   Insert

   Dppa2 (Dppa2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal CMV + TetO

   Available since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

7. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

   Plasmid

   #86843

   Purpose

   HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

   Depositor

   Thomas Cech

   Article

   Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

   Insert

   FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
   

   Use

   Tags

   FLAG-HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous TERT promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FRAP

   Plasmid

   #1827

   Depositor

   Philip Leder

   Article

   Ornitz et al Mol Cell Biol 1992 Jan;12(1):240-7.

   Insert

   FR1 AP (Fgfr1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-mas-KB-1753-Nluc I9A/W10A

   Plasmid

   #158604

   Purpose

   Plasmid expressing KB-1753-Nluc I9A/W10A in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 I9A/W10A - linker (GGTGGS) - Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-mas-GRK2RH-Nluc L118A

    Plasmid

    #158606

    Purpose

    Plasmid expressing GRK2(RH)-Nluc L118A in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain L118A - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pWPI-N1ICD

    Plasmid

    #185525

    Purpose

    Constitutive overexpression of human NOTCH1 intracellular domain

    Depositor

    Eric Shusta, Sean Palecek

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    NOTCH1 intracellular domain (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pWPI-N3ICD

    Plasmid

    #185524

    Purpose

    Constitutive overexpression of human NOTCH3 intracellular domain

    Depositor

    Eric Shusta, Sean Palecek

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    NOTCH3 intracellular domain (NOTCH3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LR (hsp70I: cPLA2-mK2)

    Plasmid

    #162564

    Purpose

    Measuring cPLA2 activation in live cells after heat shock.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Inserts

    Heat shock protein 70 (HSPA1A Human)
    cytosolic phospholipase a2 (pla2g4aa Zebrafish)
    

    Use

    Gateway donor vector

    Tags

    mK2

    Expression

    Mutation

    Promoter

    cmlc2

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ML1Nmut_in_pEGFP-C1

    Plasmid

    #67796

    Purpose

    Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFP

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    ML1N*2(7Q)-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBS-KS-attB2-SA(0)-T2A-Gal4

    Plasmid

    #62899

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBS-KS-attB2-SA(1)-T2A-Gal4

    Plasmid

    #62900

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p2T-CMV-EA-BE4max-NG-BlastR

    Plasmid

    #152992

    Purpose

    High purity C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    BE4 H47E+S48A-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBG100-TCPTP

    Plasmid

    #33365

    Depositor

    Ambra Pozzi

    Article

    Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

    Insert

    TCPTP-45 (PTPN2 Human)
    

    Use

    Tags

    6xHIS - cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1-mPCSK7

    Plasmid

    #122676

    Purpose

    Expresses mouse PCSK7 in mammalian cells

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus proprotein convertase subtilisin/kexin type 7 (Pcsk7), transcript variant 2 (Pcsk7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCG-TPMV Hd32

    Plasmid

    #163963

    Purpose

    Expresses TPMV H glycoprotein in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

    Insert

    TPMV H glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amino acids 2 to 33 at N terminus deleted

    Promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMXs3-TIAM1

    Plasmid

    #86143

    Purpose

    Retroviral vector expressing TIAM1-IRES-RFP

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p2T-CMV-eA3Amax-T31A-NG-BlastR

    Plasmid

    #153000

    Purpose

    C-to-T/G/A base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    eA3A-BE4 T31A-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)

    Plasmid

    #140226

    Purpose

    Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistance

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    Luciferase + crIFNg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV MN (eGFP)

    Plasmid

    #89932

    Purpose

    Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

    Depositor

    Carl Novina

    Article

    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

    Insert

    MN
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    M.SssI residues 2-272 fragment

    Promoter

    CMV

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLib6.4B

    Plasmid

    #176665

    Purpose

    Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RASD2

    Plasmid

    #55672

    Purpose

    expression of mouse RASD2

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RASD2 (Rasd2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

27. EK0290 SFFV-mCherry-SG(s70587-AND-s17d9b)SG-EGFP (FLP-IN)

    Plasmid

    #191143

    Purpose

    Expression of Sensor for (1 AND 2) "EK0208 AND EK0211" in mammalian cells; mCherry marker, EGFP output.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587-AND-s17d9b:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LentiCRISPR-sgC17orf89-3

    Plasmid

    #86136

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    sgRNA 3 targeting C17orf89 (NDUFAF8 )
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LentiCRISPR-sgC17orf89-1

    Plasmid

    #86137

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    sgRNA 1 targeting C17orf89 (NDUFAF8 )
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMSCV-HA-shift-IRES-GFP(KaganE21)

    Plasmid

    #52004

    Purpose

    Expresses the human MAVS CDS containing an N-terminal HA tag and a frameshift mutation inserting TA at bp number 254 of the MAVS CDS . And a GFP marker

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS-Shift
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    A 2 nucleotide insertion was introduced between t…

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only