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pDSM-cd-Psptdh3-ACO-Tagtef1

Plasmid

#127734

Purpose

Yeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psptdh3

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Ptef1-CSC-Tpdc1

Plasmid

#127739

Purpose

Yeast pathway position 6. CSC transcription unit with the TEF1 promoter and PDC1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfba1

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Pzuo1-CSC-Tvma16

Plasmid

#127740

Purpose

Yeast pathway position 6. CSC transcription unit with the ZUO1 promoter and VMA16 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pzuo1

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Ppfy1-CSC-Taip1

Plasmid

#127741

Purpose

Yeast pathway position 6. CSC transcription unit with the PFY1 promoter and AIP1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ppfy1

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Psbtdh3-CSC-Tefm1

Plasmid

#127742

Purpose

Yeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psbtdh3

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Peno2-ACDH-Ttal1

Plasmid

#127743

Purpose

Yeast pathway position 7. ACDH transcription unit with the ENO2 promoter and TAL1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Peno2

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Pvma6-ACDH-Trps14a

Plasmid

#127744

Purpose

Yeast pathway position 7. ACDH transcription unit with the VMA6 promoter and RPS14A terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pvma6

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Prpl28-ACDH-Tecm10

Plasmid

#127745

Purpose

Yeast pathway position 7. ACDH transcription unit with the RPL28 promoter and ECM10 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Prpl28

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Ptdh3-ACDH-Trpl3

Plasmid

#127746

Purpose

Yeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ptdh3

Availability

Academic Institutions and Nonprofits only

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ELP-DE30 with tyrosinase sites

Plasmid

#255773

Purpose

Expresses ELP-DE30 with tyrosinase crosslinking sites at the N-terminus

Depositor

Allie Obermeyer

Article

Fisher et al ACS Synth Biol. 2026 Feb 20;15(2):472-48

Insert

ELP-DE30 with tyrosinase sites

Expression

Bacterial

Available Since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pET22b(+)-Ptac-Ecoli_lpxP

Plasmid

#212594

Purpose

IPTG-inducible construct for the expression of lpxP under control of the 5'UTR RNA thermometer

Depositor

Danny Incarnato

Article

Borovska et al Nat Biotechnol. 2025 Jul 25. doi: 10.103

Insert

lpxP (5'UTR+CDS)

Tags

FLAG

Expression

Bacterial

Promoter

tac

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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ELP-DEQ30 with tyrosinase sites

Plasmid

#255774

Purpose

Expresses ELP-DEQ30 with tyrosinase crosslinking sites at the N-terminus

Depositor

Allie Obermeyer

Article

Fisher et al ACS Synth Biol. 2026 Feb 20;15(2):472-48

Insert

ELP-DEQ30 with tyrosinase sites

Expression

Bacterial

Available Since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet-1-PX-Gal-3-TRUNC

Plasmid

#256297

Purpose

Expresses truncated PX-Gal-3 (deletion of N-terminal domain, aa 1-116) in bacterial cells

Depositor

Mia Huang

Article

Joeh et al Proc Natl Acad Sci U S A. 2020 Nov 3;117

Insert

PX-Gal-3-TRUNC (LGALS3 Human)

Tags

His tag

Expression

Bacterial

Mutation

Deleted residues 1-116

Available Since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet-1-PX-Gal-3-MUT

Plasmid

#256296

Purpose

Expresses mutated PX-Gal-3 (R144S, R186S, G182A) in bacterial cells

Depositor

Mia Huang

Article

Joeh et al Proc Natl Acad Sci U S A. 2020 Nov 3;117

Insert

PX-Gal-3-MUT (LGALS3 Human)

Tags

His tag

Expression

Bacterial

Mutation

mutatd R144S, R186S, G182A

Available Since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pYSD328

Plasmid

#253040

Purpose

Encodes the Aga2 signal peptide, anti-ALFA-tag nanobody, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

Anti-ALFA-tag nanobody 649-stalk anchor

Expression

Yeast

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pYSD728

Plasmid

#253050

Purpose

Encodes OST1 Sig Pep, LC Cutinase-WT, FLAG-tagged SED1 surface display anchor in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

Leaf compost cutinase

Expression

Yeast

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pYSD329

Plasmid

#253041

Purpose

Encodes the Aga2 signal peptide, anti-mCherry_LaM4 nanobody, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

Anti-mCherry_LaM-4 nanobody 649-stalk anchor

Expression

Yeast

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pYSD332

Plasmid

#253042

Purpose

Encodes HA-tagged Aga2 yeast surface display anchor protein, Anti-GFP nanobody as a C-terminal fusion, in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

Aga2 Anti-GFP nanobody

Expression

Yeast

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pYSD091

Plasmid

#252981

Purpose

Encodes S. cerevisiae HA-tagged Aga1 del N181aa disp anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

HA-tagged Aga1 del N181aa display anchor

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pYSD092

Plasmid

#252982

Purpose

Encodes S. cerevisiae 2xFLAG-tagged Aga1 del N181aa disp anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

2xFLAG-tagged Aga1 del N181aa display anchor

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge