We narrowed to 77,405 results for: Mycs;

1. pKP135 (pRS406-YBR139Wp-YBR139W-PA-ADH1t)

   Plasmid

   #106470

   Purpose

   Expresses Atg42/Ybr139w with a C-terminal PA tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W
   

   Use

   Tags

   PA

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   YBR139W

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRevLCav2deltaC

   Plasmid

   #60809

   Purpose

   to make truncation of yeast Rev3

   Depositor

   Tahir Tahirov, Youri Pavlov

   Article

   Siebler et al DNA Repair (Amst). 2014 May 9. pii: S156

   Insert

   REV3 (REV3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   rev3ΔC encodes for Rev3 lacking the C-terminus (a…

   Promoter

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Gal4pBS134-Pleum

   Plasmid

   #60339

   Purpose

   Contains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

   Depositor

   Hal Alper

   Article

   Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

   Insert

   promoter
   

   Use

   Synthetic Biology; Yeast synthetic hybrid promote…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 11, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pML104

   Plasmid

   #67638

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52 and pTDH3

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pML107

   Plasmid

   #67639

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52 and pTDH3 (aka GAP promoter)

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-hAtg12

   Plasmid

   #87869

   Purpose

   Express a EGFP-human Atg12/Apg12 in mammalian cells

   Depositor

   Isei Tanida, Eiki Kominami

   Article

   Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

   Insert

   ATG12 (ATG12 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSPObooster

   Plasmid

   #216160

   Purpose

   Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains

   Depositor

   Grant Brown

   Article

   Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978

   Inserts

   MKT1(30G) (MKT1 Budding Yeast)
   RME1(ins-308A) (RME1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   MKT1 gene containing an aspartic acid to glycine …

   Promoter

   endogenous promoter

   Available since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF1a-FB-dCas9-puro

   Plasmid

   #100547

   Purpose

   Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

   Depositor

   Jian Xu

   Article

   Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

   Inserts

   N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
   puromycin
   

   Use

   CRISPR

   Tags

   FLAG, Biotin-acceptor-site (Avitag)

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Pseudojanin (PJ)

   Plasmid

   #37999

   Depositor

   Robin Irvine

   Article

   Hammond et al Science. 2012 Jun 21.

   Inserts

   FKBP1A (FKBP1A Human)
   SAC1
   INPP5E (INPP5E Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmIL-6promoterEGFP

    Plasmid

    #112896

    Purpose

    To monitor the activation of the murine IL-6 promoter by eGFP expression

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    murine IL-6 promoter (Il6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    IL-6 promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSPIH6

    Plasmid

    #190676

    Purpose

    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)

    Depositor

    John Vederas

    Article

    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi

    Inserts

    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    

    Use

    Tags

    Chitin binding domain, His tag, and N-terminal Hi…

    Expression

    Bacterial

    Mutation

    Added C-terminal his tag and None

    Promoter

    T7

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDIV515

    Plasmid

    #177703

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUG-natNT2

    Plasmid

    #110922

    Purpose

    expression of nat gene conferring resistance to nourseothricin for gene deletion in yeast

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    nat
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    AgTEF1

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD-bHs

    Plasmid

    #134259

    Purpose

    For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag

    Depositor

    Edwin Manuel

    Article

    Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7

    Insert

    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    codon optimized for Salmonella typhimurium

    Promoter

    AraC

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-hGABARAP

    Plasmid

    #87871

    Purpose

    Express a EGFP-human GABARAP in mammalian cells

    Depositor

    Isei Tanida, Eiki Kominami

    Article

    Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

    Insert

    GABARAP (GABARAP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDIV537

    Plasmid

    #177704

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pD/Paro-IFN

    Plasmid

    #205439

    Purpose

    A donor vector plasmid for Cre-mediated integration of paromomycin resistance (Paro) and dog IFNα-4 (IFNα-4) genes

    Depositor

    Masamichi Kamihira

    Article

    Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

    Inserts

    dog interferon α-4 gene
    paromomycin resistance gene
    

    Use

    Cre/Lox; Chlamydomonas expression

    Tags

    Expression

    Mutation

    Promoter

    Heat shock protein 70A (HSP70A)/ribulose bisphosp…

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSUC2::GNSI (NrsR)

    Plasmid

    #121537

    Purpose

    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.

    Depositor

    Alexey Merz

    Article

    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

    Inserts

    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    

    Use

    Tags

    mGFP5

    Expression

    Yeast

    Mutation

    M109I (Naturally occurring SNP) and The first 21 …

    Promoter

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUDP012

    Plasmid

    #101167

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEVA1

    Plasmid

    #58953

    Purpose

    Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.

    Depositor

    Thomas Fox, Thomas Mason

    Article

    Sanchirico et al EMBO J. 1998 Oct 1;17(19):5796-804.

    Insert

    VAR1u, VAR1 recoded for expression from the nucleus
    

    Use

    Yeast ecoli shuttle vector

    Tags

    preCox4 mitochondrial matrix targeting signal

    Expression

    Yeast

    Mutation

    Synthetic gene in standard genetic code

    Promoter

    ADH1

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only