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pXD80ENSF7.1-Dadh-ΔElen0469(ElA2)

Plasmid

#225315

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0469) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0469)

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.4-Dadh-ΔElen0474(ElA2)

Plasmid

#225319

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0474) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0474)

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.7-Dadh-ΔElen0477(ElA2)

Plasmid

#225322

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0477) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0477)

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.8-Dadh-ΔElen0478(ElA2)

Plasmid

#225323

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0478) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0478)

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.9-Dadh-ΔElen0481(ElA2)

Plasmid

#225324

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0481) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0481)

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.10-Dadh-ΔElen0482(ElA2)

Plasmid

#225325

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0482) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0482)

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD70DA8-DadhA(cHis8)BC-DadRS-noAccs(ElA2)

Plasmid

#225326

Purpose

Expresses Eggerthella lenta A2 Dadh in Eggerthella lenta DSM 2243

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF6-Elen0497-ElVadh-Accs(ElDSM2243)

Plasmid

#225301

Purpose

Expresses Eggerthella lenta DSM 2243 El Vadh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

El Vadh

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7-DadhA(cHis8)BC-DadRS-Accs(ElA2)

Plasmid

#225302

Purpose

Expresses Eggerthella lenta A2 Dadh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF8-HcdhA(cHis8)BC-HcdR-Accs(ElA2)

Plasmid

#225303

Purpose

Expresses Eggerthella lenta A2 Hcdh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Hcdh

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF18-C1878_02750_GsVadh(Gs28C)

Plasmid

#225304

Purpose

Expresses Gordonibacter sp. 28C Gs Vadh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Gs Vadh

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80EN3-Elen0507-Cadh-noAccs(ElA2)

Plasmid

#225311

Purpose

Expresses Eggerthella lenta A2 Cadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Cadh

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80EN4-Elen0511-(–)-Eldh1-noAccs(ElA2)

Plasmid

#225312

Purpose

Expresses Eggerthella lenta A2 (–)-Eldh1 without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh1 (Elen_0511 )

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF3-Elen0507-Cadh-Accs(ElA2)

Plasmid

#225298

Purpose

Expresses Eggerthella lenta A2 Cadh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Cadh

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD70EN25-Pct5-(–)-Eddh(Gp3C)

Plasmid

#233877

Purpose

Expresses Gordonibacter pamelaeae 3C (–)-Eddh in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-rtTA

Plasmid

#233202

Purpose

Retroviral expression of rtTA for dox-inducible activation from a TRE promoter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

rtTA

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A0_M13ps

Plasmid

#231413

Purpose

M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-A0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA47_dCas9-GFP_NOT_iA

Plasmid

#231417

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge