We narrowed to 22,610 results for: ESC;
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Plasmid#168972PurposeN-fragment of the DiB2‐split protein fused with histone H2BDepositorInsertH2B - DiB2-split N-frag
ExpressionMammalianPromoterCMVAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-N-DiB2
Plasmid#168472PurposeN-fragment of the DiB2‐split protein. This plasmid needs to be co-transformed with Plasmid 168257: pMRBad-C-wtBlc to obtain DiB2‐split proteinDepositorInsertN-fragment of DiB2
TagsHis-tagExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
1026L=U6-3-gRNA#1[Pol-γ35]
Plasmid#159674PurposePlasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.DepositorInsertU6-3-gRNA#1[Pol-γ35]
ExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
1026M=U6-3-gRNA#2[Pol-γ35]
Plasmid#159675PurposePlasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.DepositorInsertU6-3-gRNA#2[Pol-γ35]
UseCRISPRExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNL1141
Plasmid#160867PurposePJ23110-RBS-PhCFAT-mRuby2-tL3S2P24_KanR_p15ADepositorInsertConiferyl alcohol acyltransferase
UseSynthetic BiologyTagsmRuby2ExpressionBacterialPromoterPJ23110Available SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNL1127
Plasmid#160859PurposePJ23110-RBS-RhAAT1-mRuby2-tL3S2P24_KanR_p15ADepositorInsertAcetyl CoA geraniol/citronellol acetyltransferase
UseSynthetic BiologyTagsmRuby2ExpressionBacterialPromoterPJ23110Available SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD_01
Plasmid#149606PurposepTEF1-VanR inserted in pRS416 backboneDepositorInsertVanR
UseSynthetic BiologyExpressionYeastPromoterTEF1Available SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD
Plasmid#162814PurposeSTELLA plasmid for amber suppression of C-terminally labelled SMIM38DepositorInsertSMIM38(C-TAG)-Intein-DD
UsePiggybacExpressionMammalianMutationSMIM38(Ochre52,Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI
Plasmid#162803PurposeControl STELLA destination plasmid for N-terminally labelled proteinsDepositorInsertUb(G75,76A)-BsmBI
UsePiggybacExpressionMammalianMutationUbiquitin (G75,76A)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*SMIM38
Plasmid#162808PurposeSTELLA plasmid for amber suppression of N-terminally labelled SMIM38DepositorInsertUb-(N-TAG)SMIM38
UsePiggybacExpressionMammalianMutationSMIM38 (Met1, Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4x7SKPylT_EF1_Ub-*PIGBOS
Plasmid#162809PurposeSTELLA plasmid for amber suppression of N-terminally labelled PIGBOSDepositorInsertUb-(N-TAG)PIGBOS
UsePiggybacExpressionMammalianMutationPIGBOS (Met1, Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
907F1
Plasmid#160233PurposeFlightN-VanR-VP16-3X-SV40-attBDepositorInsertsVanR
Flightin promoter
TagsVP16ExpressionInsectAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - DANCER-2
Plasmid#136386PurposeExpresses DANCER-2 dynamic proteinDepositorInsertDANCER-2
Tags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L5A-L7I-A34F-V39LPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - NERD-S
Plasmid#136388PurposeExpresses NERD-S static proteinDepositorInsertNERD-S
Tags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L7I-A34F-V39I-V54IPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - NERD-C
Plasmid#136389PurposeExpresses NERD-C static proteinDepositorInsertNERD-C
Tags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L7I-F30L-V39IPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG369-lyn11-redFAST
Plasmid#160356PurposeExpresses Lyn11-redFAST in mammalian cellsDepositorInsertlyn11-redFAST
ExpressionMammalianAvailable SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG361-lyn11-greenFAST
Plasmid#160352PurposeExpresses Lyn11-greenFAST in mammalian cellsDepositorInsertlyn11-greenFAST
ExpressionMammalianAvailable SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNusGNTD
Plasmid#113123PurposeExpresses N-terminus of E. coli NusGDepositorInsertNusG
TagsHis6-MBP-N10-TEVExpressionBacterialMutationDeleted residues 120-181PromoterT7Available SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase-AntiDsRed
Plasmid#139677PurposeCassette for testing phiC31 Integrase activity using a DsRed2 fluorescent reporterDepositorInsertAntiDsRed Integrase
ExpressionPlantPromoterMaize UbiquitinAvailable SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZS039
Plasmid#134233PurposeFor in vitro transcription of Miniata CENP-ADepositorInsertMiniata CENP-A n-terminus (nt 1-204, aa 1-68)
TagsGSTExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZS077
Plasmid#134235PurposeBacterial expression of CENP-CDepositorInsertGST-CENP-C
TagsGSTExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTEI119
Plasmid#126059PurposeModule 0 vector of PlaMiNGo toolkitDepositorInsertchloroplastic FNR transit peptide
UseSynthetic Biology; Module 0Available SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI120
Plasmid#126060PurposeModule 0 vector of PlaMiNGo toolkitDepositorInsertmitochondria Rieske iron sulphur protein (1-100 aa)
UseSynthetic Biology; Module 0Available SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNOC-stacked-GeNL-Ca
Plasmid#101003PurposeConstruct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoterDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionAvailable SinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pClodANL-FGF2NBAA
Plasmid#107873PurposeBacterial expression of FGF2 minimum export mutant-NanoLucDepositorInsertFGF2-minimal export
UseLuciferaseTagsNanoLucExpressionBacterialMutationC77A, C95A, K128Q, R129Q, K134QPromoterpro DAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOEM1_pCMV:axFgf8-pPH:vsvged
Plasmid#111159PurposeBaculovirus rescue vector, VSVGED pseudotype, hShhDepositorInsertaxFgf8
UseBaculoviral rescue shuttle vectorExpressionInsect and MammalianMutationwtPromoterCMVAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCML 220
Plasmid#98588PurposepCYFP. Expresses CYFP control fusion in E. coli for TriFC assay (kanR).DepositorTypeEmpty backboneExpressionBacterialPromoterpLacOAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-ro3GFP
Plasmid#82367PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertro3GFP
Tags6xHISExpressionBacterialMutationC48S/Q80R/N149C/S202CPromoterT7Available SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE63
Plasmid#79445Purposerecipient plasmid [single nuclease]; p35S:Cas9, BASTADepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE144
Plasmid#79448Purposerecipient plasmid [multiplexing]; p35S:Cas9, FAST, BASTADepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only