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H2B-DiB2-splitN1–125

Plasmid

#168972

Purpose

N-fragment of the DiB2‐split protein fused with histone H2B

Depositor

Jens Meiler

Article

Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

Insert

H2B - DiB2-split N-frag

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-N-DiB2

Plasmid

#168472

Purpose

N-fragment of the DiB2‐split protein. This plasmid needs to be co-transformed with Plasmid 168257: pMRBad-C-wtBlc to obtain DiB2‐split protein

Depositor

Jens Meiler

Article

Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

Insert

N-fragment of DiB2

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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1026L=U6-3-gRNA#1[Pol-γ35]

Plasmid

#159674

Purpose

Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

U6-3-gRNA#1[Pol-γ35]

Expression

Insect

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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1026M=U6-3-gRNA#2[Pol-γ35]

Plasmid

#159675

Purpose

Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

U6-3-gRNA#2[Pol-γ35]

Use

CRISPR

Expression

Insect

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLNL1141

Plasmid

#160867

Purpose

PJ23110-RBS-PhCFAT-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Coniferyl alcohol acyltransferase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLNL1127

Plasmid

#160859

Purpose

PJ23110-RBS-RhAAT1-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Acetyl CoA geraniol/citronellol acetyltransferase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pVD_01

Plasmid

#149606

Purpose

pTEF1-VanR inserted in pRS416 backbone

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

VanR

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD

Plasmid

#162814

Purpose

STELLA plasmid for amber suppression of C-terminally labelled SMIM38

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

SMIM38(C-TAG)-Intein-DD

Use

Piggybac

Expression

Mammalian

Mutation

SMIM38(Ochre52,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI

Plasmid

#162803

Purpose

Control STELLA destination plasmid for N-terminally labelled proteins

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub(G75,76A)-BsmBI

Use

Piggybac

Expression

Mammalian

Mutation

Ubiquitin (G75,76A)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*SMIM38

Plasmid

#162808

Purpose

STELLA plasmid for amber suppression of N-terminally labelled SMIM38

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)SMIM38

Use

Piggybac

Expression

Mammalian

Mutation

SMIM38 (Met1, Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4x7SKPylT_EF1_Ub-*PIGBOS

Plasmid

#162809

Purpose

STELLA plasmid for amber suppression of N-terminally labelled PIGBOS

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)PIGBOS

Use

Piggybac

Expression

Mammalian

Mutation

PIGBOS (Met1, Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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907F1

Plasmid

#160233

Purpose

FlightN-VanR-VP16-3X-SV40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

VanR
Flightin promoter

Tags

VP16

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pJ414 - DANCER-2

Plasmid

#136386

Purpose

Expresses DANCER-2 dynamic protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

DANCER-2

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJ414 - NERD-S

Plasmid

#136388

Purpose

Expresses NERD-S static protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

NERD-S

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V39I-V54I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJ414 - NERD-C

Plasmid

#136389

Purpose

Expresses NERD-C static protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

NERD-C

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-F30L-V39I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAG369-lyn11-redFAST

Plasmid

#160356

Purpose

Expresses Lyn11-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

lyn11-redFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAG361-lyn11-greenFAST

Plasmid

#160352

Purpose

Expresses Lyn11-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

lyn11-greenFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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EcNusGNTD

Plasmid

#113123

Purpose

Expresses N-terminus of E. coli NusG

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

NusG

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Mutation

Deleted residues 120-181

Promoter

T7

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pIntegrase-AntiDsRed

Plasmid

#139677

Purpose

Cassette for testing phiC31 Integrase activity using a DsRed2 fluorescent reporter

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

AntiDsRed Integrase

Expression

Plant

Promoter

Maize Ubiquitin

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pZS039

Plasmid

#134233

Purpose

For in vitro transcription of Miniata CENP-A

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

Miniata CENP-A n-terminus (nt 1-204, aa 1-68)

Tags

GST

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pZS077

Plasmid

#134235

Purpose

Bacterial expression of CENP-C

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

GST-CENP-C

Tags

GST

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pTEI119

Plasmid

#126059

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

chloroplastic FNR transit peptide

Use

Synthetic Biology; Module 0

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI120

Plasmid

#126060

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Synthetic Biology; Module 0

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-GeNL-Ca

Plasmid

#101003

Purpose

Construct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis expression

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pClodANL-FGF2NBAA

Plasmid

#107873

Purpose

Bacterial expression of FGF2 minimum export mutant-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

FGF2-minimal export

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Mutation

C77A, C95A, K128Q, R129Q, K134Q

Promoter

pro D

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pOEM1_pCMV:axFgf8-pPH:vsvged

Plasmid

#111159

Purpose

Baculovirus rescue vector, VSVGED pseudotype, hShh

Depositor

Elly Tanaka

Article

Nacu et al Nature. 2016 May 19;533(7603):407-10. do

Insert

axFgf8

Use

Baculoviral rescue shuttle vector

Expression

Insect and Mammalian

Mutation

wt

Promoter

CMV

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCML 220

Plasmid

#98588

Purpose

pCYFP. Expresses CYFP control fusion in E. coli for TriFC assay (kanR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Type

Empty backbone

Expression

Bacterial

Promoter

pLacO

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro3GFP

Plasmid

#82367

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro3GFP

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/Q80R/N149C/S202C

Promoter

T7

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE63

Plasmid

#79445

Purpose

recipient plasmid [single nuclease]; p35S:Cas9, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE144

Plasmid

#79448

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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