We narrowed to 32,142 results for: eng

1. TU#1805_CRISPR_unc-73_exon2

   Plasmid

   #82359

   Purpose

   to create unc-73B null allele

   Depositor

   Martin Chalfie

   Article

   Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUP95Q15AGW (D5)

   Plasmid

   #74028

   Purpose

   lentiviral expression of Psd95-Q15A-EGFP fusion

   Depositor

   Robert Malenka, Oliver Schluter, Weifeng Xu

   Article

   Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

   Insert

   Psd95 (DLG4 Rat)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Q15A

   Promoter

   hUbC

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

3. FUP95E17RGW (D6)

   Plasmid

   #74029

   Purpose

   lentiviral expression of Psd95-E17R-EGFP fusion

   Depositor

   Robert Malenka, Oliver Schluter, Weifeng Xu

   Article

   Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

   Insert

   Psd95 (DLG4 Rat)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   E17R

   Promoter

   hUbC

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

4. FUP95DSKGW (C8)

   Plasmid

   #74024

   Purpose

   lentiviral expression of Psd95-delta SK-EGFP fusion

   Depositor

   Robert Malenka, Oliver Schluter, Weifeng Xu

   Article

   Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

   Insert

   Psd95 (DLG4 Rat)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   delta SK

   Promoter

   hUbC

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

5. tdEos-Cx43-7

   Plasmid

   #57603

   Purpose

   Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   Cx43
   

   Tags

   tdEos

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pFL-TK-AID3'UTR-DEL

   Plasmid

   #19988

   Depositor

   Nina Papavasiliou

   Article

   Teng et al Immunity. 2008 May . 28(5):621-9.

   Insert

   AID 3' UTR (Aicda Mouse)
   

   Expression

   Mammalian

   Mutation

   Deletion

   Available Since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9(BB)-2A-Puro (PX459) V2.0

   Plasmid

   #62988

   Purpose

   Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti dCAS-VP64_Blast

   Plasmid

   #61425

   Purpose

   3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Lentiviral Prep

   Insert

   dCAS9(D10A, N863A)-VP64_2A_Blast
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1A

   Available Since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGIP

   Plasmid

   #26777

   Purpose

   Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

   Depositor

   Linzhao Cheng

   Article

   Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

   Insert

   EF1a-GFP-IRES-Puro (gfp )
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3-HA-Akt1

    Plasmid

    #73408

    Purpose

    Expresses human AKT1 with a N-terminus HA tag

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. anti-FLAG M2 heavy chain

    Plasmid

    #175359

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 heavy chain
    

    Tags

    His8 tag and N-terminal secretion signal peptide …

    Expression

    Mammalian

    Mutation

    L51I (see paper)

    Promoter

    CMV

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTmpl-miniDonor-GFP

    Plasmid

    #238107

    Purpose

    Mini donor expressing GFP, used for PCR to generate IVT template.

    Depositor

    Feng Zhang

    Article

    Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

    Insert

    CMV-GFP-syntheticPA flanked by R2Tg UTRs and 28S sequence
    

    Expression

    Mammalian

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJRH-1363 LV EF1a-CD19 IRES-EGFP

    Plasmid

    #201919

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD19 (CD19 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJRH-1365 LV EF1a-CD28 IRES-EGFP

    Plasmid

    #201921

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD28

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJRH-1364 LV EF1a-CD4 IRES-EGFP

    Plasmid

    #201920

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD4

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD4 (CD4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry

    Plasmid

    #247160

    Purpose

    Engineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3

    Depositor

    Patrick Hsu

    Article

    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

    Insert

    Puromycin resistance, mCherry
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti sgRNA(MS2)_zeo backbone

    Plasmid

    #61427

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL4.20-HIF1A prom

    Plasmid

    #40173

    Depositor

    Alex Minella

    Article

    Sengupta et al Mol Cell Biol. 2011 Sep;31(18):3885-95.

    Insert

    HIF1A promoter fragment (HIF1A Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti sgRNA(MS2)_puro backbone

    Plasmid

    #73795

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSIE1

    Plasmid

    #136355

    Purpose

    Selection by Inducible Effectors

    Depositor

    Andrew Goodman

    Article

    Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

    Inserts

    Type VI effector bfe1
    Type VI effector bte1
    BT_4676
    tetR
    

    Use

    Unspecified

    Mutation

    ATG removed to add signal sequence before gene

    Promoter

    P1TDP-GHO23

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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