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pFosill-3

Plasmid

#89697

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pQacR-Q2

Plasmid

#74690

Purpose

QacR-Q2 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSQ2-QacR-PQacR-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAN4037

Plasmid

#74703

Purpose

Output plasmid: PPhlF-PBetI-YFP

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PPhlF-PBetI-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBSV2_2

Plasmid

#118226

Purpose

Empty E. coli - B. burgdorferi shuttle vector; kanamycin resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTn7xKS

Plasmid

#117393

Purpose

Tn7 tagging vector with a LacI-controlled kill switch counterselection system

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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XOR gate-V2.0

Plasmid

#44453

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

XOR-gate

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only

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pFosill-2

Plasmid

#89696

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-RILPdel212N

Plasmid

#110499

Purpose

Dominant Negative

Depositor

Terence Dermody, Bernardo Mainou

Article

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

RILPdel212N (RILP Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deleted amino terminal 212 amino acids

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBBR1-kan-Ptet-plu-gamma-ETh_rhi483

Plasmid

#180429

Purpose

Genome engineering of Agrobacterium

Depositor

Jun Fu

Article

Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

Insert

plu-gamma-ETh_rhi483

Expression

Bacterial

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFF838

Plasmid

#61457

Purpose

aTc-inducible SCRIBE_lacZ(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_lacZ(ON)

Use

Synthetic Biology

Promoter

pTetO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pKM490-Clip

Plasmid

#109281

Purpose

ORBIT integrating plasmid for C-terminal tagging with CLIP tag

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-CLIP tag

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAN4023

Plasmid

#74701

Purpose

Output plasmid: PBM3R1-YFP

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PBM3R1-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pJW1517

Plasmid

#62387

Purpose

pFA6a Osm1(M33A)-yoEGFP::LEU2

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

OSM1-EGFP fusion

Tags

EGFP

Expression

Yeast

Mutation

Methionine 33 mutated to Alanine

Promoter

Adh1p

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pSARM-luciferase

Plasmid

#67794

Purpose

Use together with VSV-G to package M-PMV reporter virus

Depositor

Stephen Goff

Article

Wang et al J Virol. 2015 Mar;89(5):2813-9. doi: 10.

Insert

firefly luciferase (gag )

Use

Luciferase

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pJW1234

Plasmid

#62367

Purpose

pRS305 pPGK1-BirA::LEU2

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA (birA )

Expression

Yeast

Promoter

PGK

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pHlyIIR-H1

Plasmid

#74681

Purpose

HlyIIR-H1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSH1-HlyIIR-PHlyIIR-YFP

Expression

Bacterial

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAmtR-A1

Plasmid

#74676

Purpose

AmtR-A1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSA1-AmtR-PAmtR-YFP

Expression

Bacterial

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pYOY178

Plasmid

#23108

Depositor

Linda Wordeman

Article

Andrews et al Dev Cell. 2004 Feb . 6(2):253-68.

Insert

Mitotic Centromere Associated Kinesin

Tags

GFP and His

Expression

Mammalian

Mutation

S92A, S106A, S108A, S112A, S186A

Available Since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pLPhygCAS9

Plasmid

#63555

Purpose

Expresses CAS9 in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

Insert

Humanized Streptococcus pyogenes Cas9 from PX330 (Addgene)

Use

Leishmania expression

Tags

flag

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pET28b-Ava3855

Plasmid

#45743

Depositor

Emily Balskus, Christopher T Walsh

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3855 (Ava_3855 Anabaena variabilis)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only