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pEGFP-C1-Nup35

Plasmid

#87342

Purpose

To express human Nup35 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

nucleoporin 35 (NUP35 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-H2GH

Plasmid

#182202

Purpose

Adeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion construct

Depositor

Joshua Kritzer

Article

Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

Insert

histone2B-HaloTag-GFP

Use

AAV

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-Gal4-Vp16-Crys-CFP

Plasmid

#97144

Purpose

For Gal4/UAS expression system

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Gal4-Vp16

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-hTimeless (pc4729)

Plasmid

#216861

Purpose

Mammalian expression vector encoding GFP tagged human Timeless

Depositor

Cristina Cardoso

Article

Prorok et al Front Cell Dev Biol. 2024 Feb 29;12:1346

Insert

Timeless (TIMELESS Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-His tagged nuclear Pif1

Plasmid

#65047

Purpose

expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

Depositor

Jean-Baptiste Boulé, Virgina Zakian

Article

Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

Insert

Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)

Tags

histag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Psy074

Plasmid

#99504

Purpose

Bacterial expression of LIPS M1+TE

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

LIPS M1+TE

Expression

Bacterial

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-mCherry

Plasmid

#73464

Purpose

Expresses mCherry for monosynaptic circuit mapping

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

mCherry

Use

Neurotropic virus

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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p415TEF cyto roGFP2-Grx1(yeast)

Plasmid

#65004

Purpose

Yeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

Insert

Glutaredoxin-1

Tags

roGFP2

Expression

Yeast

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCW57.1-3xFLAG-NLS-GFP

Plasmid

#198238

Purpose

Lentivector with tet-inducible 3xFLAG-NLS-GFP

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

3xFLAG-NLS-GFP

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_45bpLinker

Plasmid

#157791

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_45bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_mcherry

Plasmid

#167862

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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p11d-sctRPA

Plasmid

#102614

Purpose

Expression of S. cerevisiea RPA in E.coli

Depositor

Marc Wold

Article

Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

Inserts

RPA1 (RFA1 Budding Yeast)
RPA2 (RFA2 Budding Yeast)
RPA3 (RFA3 Budding Yeast)

Expression

Bacterial

Mutation

RFA2 has intron deleted

Promoter

T7

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)

Plasmid

#136627

Purpose

Plasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-LAMA-R59

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-SUN1_785

Plasmid

#125852

Purpose

Expresses SUN1_785 tagged with GFP in mammalian cells

Depositor

Miki Hieda

Article

Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

Insert

SUN1_785 (SUN1 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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Mut1-RS

Plasmid

#182881

Purpose

A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

Depositor

Miriam Amiram

Article

Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

Insert

Mut1-RS

Expression

Bacterial

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNZ18-CAH

Plasmid

#203159

Purpose

Replicates in E. coli, S. cerevisiae, and A. laidlawii

Depositor

Bogumil Karas

Article

Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

Insert

Yeast centromere, autonomously replicating sequence, histidine auxotrophic marker

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup88

Plasmid

#87338

Purpose

To express human Nup88 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP88 (NUP88 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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MBP-mmA1

Plasmid

#61090

Purpose

expression of fusion protein: MBP-hnRNP A1

Depositor

Sean Ryder

Article

Zearfoss et al RNA. 2013 Jul;19(7):948-57. doi: 10.1261

Insert

hnRNP A1 (Hnrnpa1 Mouse)

Tags

H6_MBP

Expression

Bacterial

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only
HP138-neo

Plasmid

#134247

Purpose

Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selection

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

Contains Cas9

Use

CRISPR

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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hMTP

Plasmid

#124281

Purpose

Expresses human MTP in mammalian cells.

Depositor

Mahmood Hussain

Article

Sellers et al J Biol Chem. 2003 May 30;278(22):20367-7

Insert

MTTP (MTTP Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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