Addgene

We narrowed to 25,797 results for: sta

Showing: 5541 - 5560 of 25797 results

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pET-28a mScarlet

Plasmid

#178543

Purpose

The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mScarlet

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRsetB-PercevalHR

Plasmid

#49081

Purpose

Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Use

Tags

7xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rtTA-IN

Plasmid

#60612

Purpose

Backbone for tet-inducible genes

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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miniCMV-(mNeonGreen)4-tDeg

Plasmid

#129402

Purpose

(mNeonGreen)4-tDeg fluorogenic protein

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

(mNeonGreen)-tDeg

Use

Tags

Expression

Mammalian

Mutation

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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FUGW-pHRed

Plasmid

#65742

Purpose

Lentiviral vector for pHRed expression in mammalian cells using the hUbiC promoter

Depositor

Mathew Tantama

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

pHRed

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUbiC

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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3xERRE-luciferase

Plasmid

#37851

Depositor

Rebecca Riggins

Article

Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

Insert

3 copies of ERRE upstream of luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

SV40

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT

Plasmid

#26106

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Jun 10. ():.

Type

Empty backbone

Use

Tags

6xHis, TEV cleavage site, and TrxA

Expression

Bacterial

Mutation

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5

Plasmid

#208377

Purpose

Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring

Depositor

Daniel Hawiger

Article

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

human CMV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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GST-HA-GFP11-N1

Plasmid

#91953

Purpose

Expresses GST-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

GST-HA-GFP11

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pSK9065

Plasmid

#84345

Purpose

tightly controlled expression of mRFP fusion proteins in S. aureus

Depositor

Neville Firth

Article

Brzoska et al Appl Environ Microbiol. 2013 May;79(9):3

Type

Empty backbone

Use

Tags

mRFPmars

Expression

Bacterial

Mutation

Promoter

Pxyl/tet

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pJet1.2 ermB cassette

Plasmid

#117121

Purpose

Erythromycin resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Erythromycin resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-mSA-EGFP

Plasmid

#39862

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin fused to EGFP

Use

Tags

6xHis, EGFP, and FLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NM23-H1

Plasmid

#10956

Depositor

Patricia Steeg

Article

Steeg et al J Natl Cancer Inst. 1988 Apr 6. 80(3):20

Insert

NM23 (NME1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_27_pKD46.10

Plasmid

#178922

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pME-tdTomato no stop

Plasmid

#82404

Purpose

Gateway-compatible tdTomato fusion reporter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

tdTomato (lacks stop codon)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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FRP2370

Plasmid

#127576

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pYTK-DN1

Plasmid

#180282

Purpose

Cas9 cassette (TU1)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS - pPGK1 - Cas9 - tPGK1 - ConR1

Use

Tags

Expression

Bacterial

Mutation

Promoter

pPGK1

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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FRP2365

Plasmid

#127575

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Ura3 selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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FlucWT-HA-GFP11-N1

Plasmid

#91954

Purpose

Expresses FlucWT-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucWT-HA-GFP11

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDB4581

Plasmid

#171124

Purpose

A kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.

Depositor

Li-Lin Du

Article

Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

Insert

3_sAID

Use

Other

Tags

Expression

Mutation

non applicable

Promoter

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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