164,609 results

1. p667-UBC-CDC42BPA-V5-miniTurbo_IDG-K

   Plasmid

   #189907

   Purpose

   Gateway destination clone of CDC42BPA (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   CDC42BPA (CDC42BPA Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRT498-OtUBD

   Plasmid

   #190089

   Purpose

   Expresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

   Depositor

   Mark Hochstrasser

   Article

   Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

   Insert

   OtUBD
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV4-BlaM-Vpr

   Plasmid

   #21950

   Depositor

   Warner Greene

   Article

   Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

   Insert

   BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

4. 22l-COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

   Plasmid

   #209296

   Purpose

   mitoBE expression vector, target mtRNR2 gene.

   Depositor

   Wensheng Wei

   Article

   Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

   Insert

   COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPB-CAG-Brainbow4.2-PGK-HygTK

   Plasmid

   #214126

   Purpose

   Gene expression by Piggybac transposase in mammalian cells

   Depositor

   Sho Yoshimatsu

   Insert

   Brainbow
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for mammalian cells

   Promoter

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG_smFP V5

   Plasmid

   #59758

   Purpose

   encodes multiple copies of an epitope tag V5

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   smFP _V5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TALED_Left-mouse-ATP6-1397N.

   Plasmid

   #212951

   Purpose

   Expresses TALED_Left-mouse-ATP6-1397N in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

   Insert

   TALED_Left-mouse-ATP6-1397N (ATP6 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE2 GFP-Atl1

   Plasmid

   #86679

   Purpose

   Lentivirus to stably express fluorescent protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   ATL1 (ATL1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWALIUM10-V5-TurboID-GW

   Plasmid

   #196285

   Purpose

   Gateway destination vector to express N-terminal TurboID fusion under UAS promoter, for making transgenic flies

   Depositor

   Norbert Perrimon

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-Ef1a-DIO ChETA-EYFP

    Plasmid

    #26968

    Purpose

    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    hChR2(E123T/H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T, H134R

    Promoter

    EF1alpha

    Available Since

    Jan. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3 HA Arf6

    Plasmid

    #10834

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ALKBH5-dCas9

    Plasmid

    #134783

    Purpose

    Expression fusion protein ALKBH5-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    ALKBH5 and inactive dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMAL-his-LbCpf1-EC

    Plasmid

    #79008

    Purpose

    Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    LbCpf1
    

    Use

    Tags

    MBP-6xHis-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. cyto-CFP-FRBx5

    Plasmid

    #103776

    Purpose

    expresses iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    cyto-CFP-FRBx5
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO5.sgRNA.EFS.GFP

    Plasmid

    #57822

    Purpose

    3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO.1-TetON-Puro-HIF1a 3809

    Plasmid

    #118705

    Purpose

    Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    shRNA against human HIF1a
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. cyto-YFP-FKBPx5

    Plasmid

    #103777

    Purpose

    expression of iPOLYMER component in mammalian cells; has nuclear exclusion sequence

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    cyto-YFP-FKBPx5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TALED_Right-Cox3.1-1397C-AD.

    Plasmid

    #212953

    Purpose

    ExpressesTALED_Right-Cox3.1-1397C-AD in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

    Insert

    TALED_Right-Cox3.1-1397C-AD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TALED_Left-Cox3.1-1397N.

    Plasmid

    #212950

    Purpose

    Expresses TALED_Left-Cox3.1-1397N. in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

    Insert

    TALED_Left-Cox3.1-1397N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-ADAM10-HA

    Plasmid

    #65106

    Purpose

    Expression of HA tagged ADAM10 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    ADAM10 (ADAM10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge