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pSW068

Plasmid

#140036

Purpose

KO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Truncated thioesterase gene

Expression

Bacterial

Promoter

cLac143

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHKMC1: Empty sgRNA for Cloning

Plasmid

#67720

Purpose

Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.

Depositor

Monica Colaiacovo

Article

Norris et al Genetics. 2015 Oct;201(2):449-58. doi: 1

Type

Empty backbone

Expression

Worm

Promoter

PU6

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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PU6::klp-12_sgRNA

Plasmid

#46170

Purpose

klp-12 targeting sgRNA

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

klp-12 targeting sgRNA (klp-12 Synthetic)

Use

CRISPR

Expression

Worm

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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p153 pPr-cre

Plasmid

#8403

Purpose

Expression of Cre from tissue-specific, androgen-inducible probasin promoter.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

probasin driven Cre

Use

Cre/Lox

Expression

Mammalian

Promoter

probasin promoter

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
GST-Cav FL (1-178)

Plasmid

#22445

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin1(1-178)

Tags

GST

Expression

Bacterial

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1_35S-eGFP-T35S (GB4279)

Plasmid

#225434

Purpose

Transcriptional unit for constitutive expression of the enhanced GFP.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

35S:eGFP:T35S

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-agrBD-I

Plasmid

#53439

Purpose

T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrBD locus

Use

Synthetic Biology

Tags

V5 epitope, 6x HIS (not expressed on agrB or agrD…

Expression

Bacterial

Mutation

none

Promoter

T7-lac

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pSZT025

Plasmid

#140033

Purpose

KO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Truncated thioesterase gene

Expression

Bacterial

Promoter

cLac143

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAG260

Plasmid

#33210

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

SYNM (SYNM Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

SV40 early enhancer/promoter

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pZG71

Plasmid

#202577

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-dADARcd-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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phNFL-Q332P

Plasmid

#132599

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E Q332P mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Human)

Expression

Mammalian

Mutation

995A>C;Gln332Pro

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-P8R-EGFP

Plasmid

#132598

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P8R mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

23C>G; Pro8Arg

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-N98S-EGFP

Plasmid

#132600

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

293A>G; Asn98Ser

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-E397K-EGFP

Plasmid

#132603

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E E397K mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

1189G>A; Glu397Lys

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pHFX-B60-EPSUD

Plasmid

#174390

Purpose

Plasmids for gene knockout were constructed based on suicide plasmid pHFX-B60

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

EPSUD

Use

Archaeal gene knockout vector for haloferax medit…

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMJA053

Plasmid

#128515

Purpose

Expresses SFA8 in Pichia

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2002 Nov 15;324(1):165-75.

Insert

SFA8 a 2S albumin from sunflower (LOC110892047 Helianthus annus)

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-IV

Plasmid

#53438

Purpose

xylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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GST-Cav (61-101)

Plasmid

#22447

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin1 (61-101)

Tags

GST

Expression

Bacterial

Available Since

Jan. 26, 2010

Availability

Academic Institutions and Nonprofits only
GST-Cav (135-178)

Plasmid

#22448

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin 1

Tags

GST

Expression

Bacterial

Mutation

aa 135-178

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only
pUAP1_Ta_Yr7_CDS_no_STOP

Plasmid

#141093

Purpose

Level 0 Golden gate module with the coding sequence of Cadenza-Yr7 without its STOP codon

Depositor

Cristobal Uauy

Article

Marchal et al Genes (Basel). 2020 Nov 26;11(12). pii:

Insert

Ta_Yr7

Use

Unspecified; Level 0 module for golden gate cloni…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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