We narrowed to 10,611 results for: t7

1. pET15T+1X

   Plasmid

   #108188

   Purpose

   PCNA1-PdX fusion protein

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PCNA1-PdX fusion protein
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   C73S/C85S (PdX)

   Promoter

   T7 promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-FFSARG-ddFLN4-HIS-ybbr

   Plasmid

   #101240

   Purpose

   minimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   FFSARG peptide, ddFLN4 fingerprint
   

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTH861-gst-optec P210T

   Plasmid

   #89595

   Purpose

   Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
   

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed proline 210 to threonine

   Promoter

   T7

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTH860-gst-optec A180Q

   Plasmid

   #89594

   Purpose

   Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
   

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed alanine 180 to glutamine

   Promoter

   T7

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTH858-gst-optec V156E

   Plasmid

   #89592

   Purpose

   Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
   

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed valine 156 to glutamic acid

   Promoter

   T7

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTH859-gst-optec Y172M

   Plasmid

   #89593

   Purpose

   Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
   

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed tyrosine 172 to methionine

   Promoter

   T7

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-Metavinculin tail

   Plasmid

   #107152

   Purpose

   bacterial expression

   Depositor

   Tina Izard

   Article

   Chinthalapudi et al Proc Natl Acad Sci U S A. 2016 Aug 23;11

   Insert

   metavinculin (VCL Human)
   

   Tags

   (His)8

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-PF2050

   Plasmid

   #100721

   Purpose

   Expression of Pyrococcus furiosus PF2050

   Depositor

   Byung Il Lee

   Article

   Han et al FEBS Lett. 2012 May 7;586(9):1384-8. doi

   Insert

   PF2050
   

   Expression

   Bacterial

   Promoter

   T7 promotor

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. CTL2

   Plasmid

   #92212

   Purpose

   Expresses C-Terminal segment of C. elegans Piezo1 in E. coli

   Depositor

   Douglas Rees

   Article

   Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

   Insert

   CTL2 of PIEZO1
   

   Tags

   6x Histidine Tag

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRAF-MutL(R95F)

    Plasmid

    #86608

    Purpose

    E. coli Mutl(R95F) expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutL(R95F)
    

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    R95F

    Promoter

    T7

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. JPUB_006818

    Plasmid

    #87924

    Purpose

    E. coli expression of K. stuttgartiensis protein

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    Kuste3608
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. JPUB_006812

    Plasmid

    #87925

    Purpose

    E. coli expression of K. stuttgartiensis protein

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    Kuste2803
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPD1cR

    Plasmid

    #86970

    Purpose

    Expresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, G108C in PCNA1

    Promoter

    T7 promoter

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPD1R

    Plasmid

    #86969

    Purpose

    Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH

    Promoter

    T7 promoter

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPD2cX

    Plasmid

    #86968

    Purpose

    Expresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…

    Promoter

    T7 promoter

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGS-3

    Plasmid

    #85094

    Purpose

    Host vector to fuse a gene at the N-terminus of PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC3

    Plasmid

    #85087

    Purpose

    Expresses PCNA3 protein fused to P450cam (CYP101A1) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    PCNA3 fused to P450cam
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSETB-ro3GFP

    Plasmid

    #82367

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro3GFP
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C48S/Q80R/N149C/S202C

    Promoter

    T7

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK040 (pET-PurQ-A128T)

    Plasmid

    #73840

    Purpose

    Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Article

    Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

    Insert

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    

    Expression

    Bacterial

    Mutation

    A128T mutant

    Promoter

    T7

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK591

    Plasmid

    #71589

    Purpose

    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala/Asp177>Ala mutants (UctC-T2A,D177A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

    Insert

    acetyl-CoA:oxalate CoA-transferase
    

    Expression

    Bacterial

    Mutation

    changes threonine-2 to alanine and aspartate-177 …

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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