We narrowed to 2,368 results for: pcas9

1. PET-SpCas9-HF1-NLS-6xHis

   Plasmid

   #207382

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET-B-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207378

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A, amino acids 1005-1013 replaced with two gl…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-Blackjack SpCas9-NLS-6xHis

   Plasmid

   #207375

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Blackjack SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   amino acids 1005-1013 replaced with two glycine

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA23-sgRNA vector-U6-sgTS2 (SpCas9)

   Plasmid

   #199454

   Purpose

   U6-driven sgRNA targeting TS2 sequence (GGAGCTTACTGAGACTCTTC)

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   TS2 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   mouse U6

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA17-CRISPR-Tag (11XTS1) for dSpCas9

   Plasmid

   #199448

   Purpose

   CRISPR-Tag sequence that can be efficiently bound by dSpCas9

   Depositor

   Baohui Chen

   Article

   Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

   Insert

   CRISPR-Tag
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-gRNA(SFTPCI73Tcorr)-SpCas9(BB)-2A-GFP

   Plasmid

   #187647

   Purpose

   Encodes gRNA to correct the SFTPC I73T mutation

   Depositor

   Darrell Kotton

   Article

   Alysandratos et al Cell Rep. 2021 Aug 31;36(9):109636. doi:

   Insert

   I73T gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

   Plasmid

   #161924

   Purpose

   To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   sgRNA targeting RDH11 exon 2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJCC_162 SpCas9 KES(1107-1109)GG

   Plasmid

   #179526

   Purpose

   For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 KES(1107-1109)GG
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   replaced KES (residues 1107-1109) with GG

   Promoter

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pspCas9-3exo-Tetra-com-CLCN5-sp-g1

   Plasmid

   #176236

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

    Plasmid

    #176237

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pspCas9-Exo-tetra-com-hCLCN5-sp-g1

    Plasmid

    #176238

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. eSpCas9(1.1)-ABL1-gRNA1-exon4

    Plasmid

    #163322

    Purpose

    eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ABL1 gRNA 1_Exon4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

13. eSpCas9(1.1)-ABL1-gRNA2-exon4

    Plasmid

    #163323

    Purpose

    eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ABL1 gRNA 2_Exon4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

14. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

    Plasmid

    #165083

    Purpose

    gRNA 2 of pair B for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

    Plasmid

    #165085

    Purpose

    gRNA 1 of pair C for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

    Plasmid

    #165086

    Purpose

    gRNA 2 of pair C for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX459V2.0-eSpCas9(1.1)+K810A+K855A

    Plasmid

    #108299

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K855A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid

    #140001

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

    Plasmid

    #213969

    Purpose

    AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    Shank3 sgRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT3TS-spCas9 ABE-Umax-ex2-rest1

    Plasmid

    #222143

    Purpose

    Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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