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Showing: 811 - 840 of 28353 results

  2. pAG426-GAL-Prepro-3x-Adan

    Plasmid
    #181733
    Purpose
    Galactose inducible expression of Prepro-3x-Adan
    Depositor
    Alejandro Chavez
    Insert
    Prepro-3x-Adan
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    181733-plasmid-map-sequence-id-431125

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  5. pRSF-DUET-C17orf53_1-87-Strep

    Plasmid
    #211433
    Purpose
    bacterial expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to a Strep-Tag
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    Strep-Tag
    Expression
    Bacterial
    Mutation
    Deletion of aa 88-646
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211433-plasmid-map-sequence-id-420530

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  6. pUC-ODN-hsRPL3-CterTwStrep

    Plasmid
    #208644
    Purpose
    for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.
    Depositor
    Quanfu Ma
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
    Use
    CRISPR; Ssdna donnor preparation
    Tags
    Twin Strep
    Expression
    Mutation
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208644-plasmid-map-sequence-id-418358

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  7. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

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  8. pFsynW SYT1-PGM reporter

    Plasmid
    #197278
    Purpose
    Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYT1 and HaloTag (Syt1 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A
    Promoter
    hSyn (human synapsin I promoter)
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197278-plasmid-map-sequence-id-389096

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  9. pOET3-C-TEV-Twin-Strep

    Plasmid
    #203324
    Purpose
    Generate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and Twin-strep-tag
    Expression
    Insect
    Mutation
    Promoter
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    203324-plasmid-map-sequence-id-399463

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  17. pN23-LIC-FLAG-STREP

    Plasmid
    #182508
    Purpose
    pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and STREP
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Use
    Tags
    STREP-FLAG-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    182508-plasmid-map-sequence-id-361393

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  27. pACEBac1-Q22YU3 del C3-2xStrep

    Plasmid
    #170316
    Purpose
    Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.
    Depositor
    Andrew Carter
    Article
    Mali et al Science. 2021 Feb 26;371(6532):910-916.
    Insert
    Q22YU3 C-terminal truncation (Delta-C3)
    Use
    Tags
    Strep-Tag II
    Expression
    Insect
    Mutation
    Deletion of C-terminal C3-finger
    Promoter
    polyhedrin
    Available since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    170316-plasmid-map-sequence-id-333816

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Showing: 811 - 840 of 28353 results