We narrowed to 1,344 results for: SUMO

Showing: 811 - 840 of 1344 results

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GFP-ataxin-3

Plasmid

#89975

Purpose

Expresses GFP-tagged ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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NP-11-63

Plasmid

#234358

Purpose

Expressing and purifying R.rubrum rubisco using a his-sumo tag

Depositor

David Savage

Article

Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

Insert

R.rubrum rubisco

Use

Synthetic Biology

Tags

N-term SUMO tag

Promoter

T7

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-pcDNA3.1

Plasmid

#85760

Purpose

mammalian expression of full length HA tagged USPL1

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length (USPL1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP1B 1-199

Plasmid

#115326

Purpose

Expresses residues 1-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-24

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Tags

His-SUMO

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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hHSF1 S303/7A

Plasmid

#118365

Purpose

This plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S303A and S307A

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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USPL1cat-pETM30

Plasmid

#85768

Purpose

bacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusion

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, catalytic domain (aa 212-514) (USPL1 Human)

Tags

GST and His

Expression

Bacterial

Mutation

catalytic domain (aa 212-514)

Promoter

T7-lac

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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hGATA-1 K137R

Plasmid

#118353

Purpose

This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hGATA-1 (GATA1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K137R

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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hHSF1 K298R

Plasmid

#118362

Purpose

This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K298R

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pInducer10-UBC9-sh4

Plasmid

#168988

Purpose

Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Ubiquitin Conjugating Enzyme 9

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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USPL1cat-pEYFP_C1

Plasmid

#85766

Purpose

mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, catalytic domain (aa 212-514) (USPL1 Human)

Tags

EYFP

Expression

Mammalian

Mutation

catalytic domain (aa 212-514)

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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hHSF1 S303A

Plasmid

#118363

Purpose

This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S303A

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYO1261

Plasmid

#235726

Purpose

Protein expression of His6-SUMO-human RAB5A Q79L 1-211 in bacteria

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

RAB5A (RAB5A Human)

Tags

6xHis-SUMO-TEV

Expression

Bacterial

Mutation

Q79L, aa 1-211

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMDS1

Plasmid

#239460

Purpose

For simultaneous monitoring of both transcription and translation of genes in plant tissues: C-terminus fusion

Depositor

Miguel de Lucas

Article

Banda et al Sci. Adv., 11, eadw9153 (2025)

Type

Empty backbone

Tags

VENUS:3xHA:2A:mTurqN7

Expression

Bacterial and Plant

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMDS2

Plasmid

#239459

Purpose

Simultaneous monitoring of transcription and translation of genes in plant tissues: N- terminus fusion

Depositor

Miguel de Lucas

Article

Banda et al Sci. Adv., 11, eadw9153 (2025)

Type

Empty backbone

Tags

3xHA:VENUS:2A:mTurqN7

Expression

Bacterial and Plant

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-SLD1

Plasmid

#210264

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-SLD1. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-SLD1 (delta aa 260-335) (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

delta-SLD1

Promoter

CMV/TetO2

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 D394R

Plasmid

#210265

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 D394R siRES (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

D394R, siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-N1

Plasmid

#210268

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-N1 resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-N1 (aa 208-419) siRES (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

delta-N1, siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-N2

Plasmid

#210269

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-N2 resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-N2 (aa 261-419) siRES (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

delta-N2, siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-NIP45 D394R

Plasmid

#210267

Purpose

Inducible expression of N-terminally mCherry-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 D394R siRES (NFATC2IP Synthetic)

Tags

mCherry

Expression

Mammalian

Mutation

D394R, siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-NIP45 wt

Plasmid

#210266

Purpose

Inducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 wt siRES (NFATC2IP Synthetic)

Tags

mCherry

Expression

Mammalian

Mutation

siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-SLD2

Plasmid

#210263

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-SLD2. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-SLD2 (aa 1-343) (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

delta-SLD2

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 wt

Plasmid

#210262

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 wt siRES (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP4C, residues 156-233, no Cys

Plasmid

#180642

Purpose

Bacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CHMP4C (CHMP4C Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

residues 316-366

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1, residues 315-360, no Cys

Plasmid

#180643

Purpose

Bacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

residues 315-360

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 KATNA1 MIT residues 1-79

Plasmid

#180614

Purpose

Bacterial expression for KATNA1 MIT domains, residues 1-79. Has N-terminal HIS-SUMO tag. Internal ID: WISP19-20.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-79

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 KATNA1 MIT residues 1-79, V55D

Plasmid

#180615

Purpose

Bacterial expression for KATNA1 MIT domains, residues 1-79. Has V55D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-23.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-79, V55D mutation

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 KATNAL1 MIT residues 1-87

Plasmid

#180618

Purpose

Bacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNAL1 (KATNAL1 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-87

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP1B 4-199

Plasmid

#115325

Purpose

Expresses residues 4-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-23

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Tags

His-SUMO

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pInducer10-UBC9-sh3

Plasmid

#168987

Purpose

Expresses RFP cDNA and UBC9 shRNA 3 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Ubiquitin Conjugating Enzyme 9

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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SP100A-c082

Plasmid

#53635

Purpose

bacterial expression plasmid

Depositor

Nicola Burgess-Brown

Insert

SP100A

Tags

His-6-SUMO

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Promoter

T7

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only