We narrowed to 23,118 results for: Sis

Showing: 1081 - 1120 of 23118 results

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pET19b_4CL:P3S

Plasmid

#139796

Purpose

expression of biosynthetic enzyme 4CL in fusion with CC segment P3S

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

4CL:P3S

Expression

Bacterial

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pNOC-FLUC

Plasmid

#98140

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven firefly luciferase reporter, hygromycin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGGE003

Plasmid

#48854

Purpose

Provides the At1g04880 terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

At1g04880 terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGE002

Plasmid

#48840

Purpose

Provides the WUS terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

WUS terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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UB13p::dsSPL10 v01 (SPL10-RNAi)

Plasmid

#27409

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 inverted (AT1G27370 Mustard Weed)

Available Since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only

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C205

Plasmid

#174359

Purpose

For expressing siRNA-resistant frog ePAB in human cell lines via transfection

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

ePAB

Tags

FLAG

Expression

Mammalian

Mutation

Multiple silent mutations introduced to be resist…

Availability

Academic Institutions and Nonprofits only

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pSAR-MT-APC

Plasmid

#16487

Depositor

Bert Vogelstein

Article

Morin et al Proc Natl Acad Sci U S A. 1996 Jul 23. 9

Insert

APC (APC Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
BCJJ345

Plasmid

#117504

Purpose

YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtYAO

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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BCJJ125

Plasmid

#117515

Purpose

Cas9_3 (with intron) Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:AATG:Cas9_3(intron):GCTT:BsaI

Use

CRISPR

Tags

2xFLAG and NLS

Expression

Plant

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pZA69

Plasmid

#174782

Purpose

RNAi

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

Intron-containing hairpin RNA (ihpRNA)-NbZAR1

Use

RNAi

Mutation

WT

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGGF007

Plasmid

#48847

Purpose

Provides a kanamycin resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pNOS::KanamycinR::tNOS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLRD13

Plasmid

#208265

Purpose

Production of ent-cassa-12,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL7

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD15

Plasmid

#208267

Purpose

Production of ent-sandaracopimaradiene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL10

Expression

Bacterial

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGGF005

Plasmid

#48846

Purpose

Provides a hygromycin resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pUBQ10::HygromycinR::tOCS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pYC1876

Plasmid

#158722

Purpose

Helper plasmid pYC1640 for C-terminal tagging with degradation tag ssrA

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

ssrA

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET24c(+)-mtEFTu

Plasmid

#30535

Depositor

Linda Spremulli

Article

Woriax et al Biochim Biophys Acta. 1995 Dec 27. 1264(

Insert

Mature bovine mitochondrial elongation factor Tu

Tags

His

Expression

Bacterial

Mutation

Mature form amino acids 44-452. Mito import seque…

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pGGF003

Plasmid

#48844

Purpose

Provides a D-alanine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::D-AlanineR::tMAS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSKB2-TRex

Plasmid

#31349

Depositor

Clara Kielkopf

Article

McLaughlin et al Mol Cell. 2010 May 28. 38(4):563-75.

Insert

Thermus aquaticus Redox Sensing Repressor

Tags

His

Expression

Bacterial

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pNOC-stacked-GeNL

Plasmid

#101002

Purpose

Construct for fusion expression of GeNL reporter and neomycin/G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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BCJJ180

Plasmid

#117516

Purpose

Cas9_4 Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:AATG:Cas9_4:GCTT:BsaI

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Plant

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL90016

Plasmid

#117514

Purpose

Cas9_2 Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:AATG:Cas9_2:GCTT:BsaI

Use

CRISPR

Tags

NLS_SV40

Expression

Plant

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pGGF001

Plasmid

#48842

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::BastaR::tMAS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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BCJJ358

Plasmid

#117505

Purpose

RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtRPS5a

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-CsCYP722C(pYL877)

Plasmid

#178296

Purpose

Expresses CsCYP722C from C. sinensis in Saccharomyces cerevisiae. Centromeric TRP, PGPD-CsCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

CsCYP722C

Expression

Yeast

Promoter

GAP

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-ClbN-NHis

Plasmid

#51497

Purpose

NRPS module containing CATE domains with N-terminal 6His tag

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbN

Tags

6x His tag and T7 tag

Expression

Bacterial

Mutation

contains aa30-1094 of NP_754347.1

Promoter

T7

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pET15b-At 14-3-3 nu

Plasmid

#44940

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 nu (GRF7 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 mu

Plasmid

#44938

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 mu (GRF9 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
SPL10p::pCGTAG (SPL10p::NLS-GFP)

Plasmid

#27410

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 promoter (AT1G27370 Mustard Weed)

Mutation

SPL10 promoter transcriptionally fused to a nucle…

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pZA70

Plasmid

#174784

Purpose

RNAi

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

Intron-containing hairpin RNA (ihpRNA)-NbJIM2

Use

RNAi

Mutation

WT

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJH123

Plasmid

#172146

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.B.(SD4)CD

Expression

Bacterial

Mutation

PylBCD operon (RBS inserted for PylC)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD111

Plasmid

#172957

Purpose

pRMCD77 encoding Wzz mutant G311A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G311A mutant gene

Expression

Bacterial

Mutation

D168G and N33D- please see depositor comment

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD113

Plasmid

#172959

Purpose

pRMCD77 encoding Wzz mutant G305A/G311A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G305A/G311A mutant gene

Expression

Bacterial

Mutation

G305A/G311A, A208V - please see depositor comments

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b_STS:P4S

Plasmid

#139797

Purpose

expression of biosynthetic enzyme STS in fusion with CC segment P4S

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

STS:P4S

Expression

Bacterial

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK349

Plasmid

#71566

Purpose

Produces Helianthus annuus delta12 fatty acid acetylenase (vFAD2) in yeast

Depositor

T. J. Kappock

Article

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid acetylenase

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK272

Plasmid

#71562

Purpose

Produces Crepis alpina delta12 fatty acid acetylenase (vFAD2) in yeast

Depositor

T. J. Kappock

Article

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid acetylenase

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET29b-ClbP-S95A

Plasmid

#48245

Purpose

Untagged, full-length protein with active site mutation Serine95 to Alanine for in vivo peptidase assays

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP

Expression

Bacterial

Mutation

Changed Serine 95 to Alanine

Promoter

T7

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGA003

Plasmid

#48814

Purpose

Provides the WUS promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

WUS promoter

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
zw4 (egghead) cDNA#7

Plasmid

#37398

Depositor

Norbert Perrimon

Article

Goode et al Development. 1996 Dec;122(12):3863-79.

Insert

egghead (egh Fly)

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only
zw4 (egghead) cDNA#4e

Plasmid

#37399

Depositor

Norbert Perrimon

Article

Goode et al Development. 1996 Dec;122(12):3863-79.

Insert

egghead (egh Fly)

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
6mSPL11F15R14::pBIB-BAS (6mSPL11)

Plasmid

#27387

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL11 mutant (AT1G27360 Mustard Weed)

Use

Used to create transgenic lines

Mutation

miR156 target sites of SPL11 mutated

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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Showing: 1081 - 1120 of 23118 results

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We narrowed to 23,118 results for: Sis