Addgene

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Showing: 1081 - 1120 of 2761 results

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miR-124-5 promoter GFP

Plasmid

#20792

Depositor

David Bartel

Article

Shkumatava et al Genes Dev. 2009 Feb 15. 23(4):466-81.

Insert

mir-124 (mir124-5 Zebrafish)

Use

Zebrafish expression

Tags

GFP

Expression

Mutation

Promoter

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only
pCON1-ProA.mCherry

Plasmid

#112519

Purpose

ProA driven mCherry expression with synTT

Depositor

Olivia Steele-Mortimer

Article

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

ProA mCherry TT

Use

Tags

Expression

Bacterial

Mutation

Promoter

ProA

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
UbcH10 DN

Plasmid

#8505

Depositor

Joan Ruderman

Article

Townsley et al Proc Natl Acad Sci U S A 1997 Mar 18;94(

Insert

UbcH10 (UBE2C Human)

Use

Tags

Expression

Bacterial

Mutation

C114S, L118S

Promoter

Available since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
pCON1-ProC.mCherry

Plasmid

#112521

Purpose

ProC driven mCherry expression with synTT

Depositor

Olivia Steele-Mortimer

Article

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

ProC mCherry TT

Use

Tags

Expression

Bacterial

Mutation

Promoter

ProC

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCON1-ProA.gfp

Plasmid

#112515

Purpose

ProA driven gfpmut3 expression with synTT

Depositor

Olivia Steele-Mortimer

Article

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

ProA gfpmut3 TT

Use

Tags

Expression

Bacterial

Mutation

Promoter

ProA

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
UbcH10

Plasmid

#8504

Depositor

Joan Ruderman

Article

Townsley et al Proc Natl Acad Sci U S A 1997 Mar 18;94(

Insert

UbcH10 (UBE2C Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
PL06010B1H03 (smedwi1)

Plasmid

#35699

Depositor

Phillip Newmark

Article

Zayas et al Proc Natl Acad Sci U S A. 2005 Dec 20;10

Insert

Smedwi1

Use

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pUC-TalO8 (Blank-CoTrIP)

Plasmid

#178303

Purpose

Blank CoTrIP plasmid

Depositor

Brian Zid

Article

Chen et al Elife. 2022 Sep 15;11. pii: 76965. doi:

Type

Empty backbone

Use

Tags

NA

Expression

Yeast

Mutation

Promoter

NA

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH39

Plasmid

#126013

Purpose

Level0 Rice OsU3 Pol III promoter with transcription Start-A

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Rice OsU3 Pol III promoter with transcription Start-A

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
nm-mhc-b

Plasmid

#78709

Purpose

Encodes zebrafish nm-mhc-b

Depositor

Sarah Childs

Article

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

nm-mhc-b (myh10 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
pJK227

Plasmid

#71126

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Use

Tags

GyrA intein

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK226

Plasmid

#71125

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK136

Plasmid

#71122

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AarA) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK376

Plasmid

#70235

Purpose

Protein production from a synthetic gene encoding Treponema denticola ATCC 35405 PurE (UniProt Q73PV9_TREDE)

Depositor

T. J. Kappock

Article

Tranchimand et al Biochemistry. 2011 May 31;50(21):4623-37

Insert

phosphoribosylaminoimidazole carboxylase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PL06010B1E06 (smedwi3)

Plasmid

#35697

Depositor

Phillip Newmark

Article

Zayas et al Proc Natl Acad Sci U S A. 2005 Dec 20;10

Insert

Smedwi3

Use

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
FKBP-GFP-Sec61β

Plasmid

#172442

Purpose

Expression of ER protein Sec61beta with an N-terminal FKBP-GFP tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Protein transport protein Sec61 subunit beta

Use

Tags

FKBP-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag Rab12 Q101L

Plasmid

#86788

Purpose

mammalian expression of Rab12 Q101L, constitutively GTP-bound mutant

Depositor

Peter McPherson

Article

Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

Insert

Rab12 (RAB12 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Q101L, constitutively GTP-bound mutant

Promoter

CMV

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag Rab12 T56N

Plasmid

#86789

Purpose

mammalian expression of Rab12 T56N, constitutively GDP-bound mutant

Depositor

Peter McPherson

Article

Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

Insert

Rab12 (RAB12 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

T56N, constitutively GDP-bound mutant

Promoter

CMV

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-supervillin

Plasmid

#13040

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pMKV057

Plasmid

#133312

Purpose

BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, WUS2 and IPT

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc+. WUS2, IPT

Use

Tags

Expression

Plant

Mutation

Promoter

AtUbi10, Nos, 35S

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM117

Plasmid

#127212

Purpose

BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, sgRNA

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4-Flag-Timeless

Plasmid

#22887

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2005 Apr . 25(8):3109-16.

Insert

Timeless (TIMELESS Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted normal start codon, replaced with start c…

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_210d

Plasmid

#91109

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-6-StayGold-9

Plasmid

#197734

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

StayGold GFP

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_220

Plasmid

#91113

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-BAF

Plasmid

#172447

Purpose

Expression of nuclear protein BAF with an N-terminal mCherry tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

BAF

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Sec61β

Plasmid

#172445

Purpose

Expression of ER protein Sec61beta with an N-terminal mCherry tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Protein transport protein Sec61 subunit beta

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-6-IPT-9

Plasmid

#197746

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Isopentyl transferase (IPT)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_220d

Plasmid

#91114

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
wg186Fluc

Plasmid

#177436

Purpose

In vitro synthesis of an mRNA encoding Firefly luciferase with A[62] tail.

Depositor

Wendy Gilbert

Article

Rojas-Duran et al RNA. 2012 Dec;18(12):2299-305. doi: 10.1

Insert

Firefly luciferase

Use

Luciferase; For in vitro transcription

Tags

None

Expression

Mutation

Promoter

T7

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRN114

Plasmid

#127221

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, Wus, AtSTM

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_210

Plasmid

#91108

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_201

Plasmid

#91105

Purpose

Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_C0000

Plasmid

#91081

Purpose

Module C, Promoter: none, Gene: none (BaeI site for GT donor cloning), Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_260d

Plasmid

#91126

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

PvUbi2

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL1 eforRed

Plasmid

#106320

Purpose

Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

eforRed

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_150

Plasmid

#91205

Purpose

Transformation, Type: non-T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_100

Plasmid

#91198

Purpose

Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_A3001

Plasmid

#91042

Purpose

Module A, Promoter: 35S, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Tags

Expression

Mutation

Promoter

35S

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_B0000

Plasmid

#91058

Purpose

Module B, Promoter: none, Gene: none, Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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