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Plasmid#112348PurposeCRISPR donor plasmid to tag human transcription factor ZBTB11 with GFPDepositorInsertZBTB11 homology arms flanking EGFP-IRES-Neo cassette (ZBTB11 Human)
UseCRISPRTagsExpressionMutationhg38:3:101651181:A>TPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVEZF1.1.0-gDNA
Plasmid#112437PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor VEZF1DepositorInsertVEZF1 (VEZF1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZFX-donor
Plasmid#112366PurposeCRISPR donor plasmid to tag human transcription factor ZFX with GFPDepositorInsertZFX homology arms flanking EGFP-IRES-Neo cassette (ZFX Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP3TP
Plasmid#112604PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and thioredoxin fusionsDepositorTypeEmpty backboneUseTagsAvi - 8His-thioredoxinExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Halo RagB
Plasmid#112753Purposeexpress Halo-RagBDepositorInsertHalo-RagB (RRAGB Human)
UseTagsHaloExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP3TT
Plasmid#112605PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and thioredoxin fusionsDepositorTypeEmpty backboneUseTagsAvi - 8His-thioredoxinExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAA.3.lacIh_I12X86
Plasmid#112025PurposeExpresses LacI_I12X86 proteinDepositorInsertLacI_I12X86
UseTagshisExpressionMutationPromoterT7Available sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
gRNA[Sxl].1026B
Plasmid#112688Purposeexpress gRNA targeting Sxl under dU6-3 promoterDepositorInsertU6.3-gRNA[Sxl] (Sxl Fly)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-hRIPK1-Flag-K115R
Plasmid#112488PurposeMammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.DepositorInsertRIPK1 (RIPK1 Human)
UseTagsFlagExpressionMammalianMutationK115RPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM549
Plasmid#112531PurposepZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpRDepositorInsertsHrtR
ChuA
UseTagsExpressionBacterialMutationPromoterJ23107 and ProDAvailable sinceFeb. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOP3BP
Plasmid#112602PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and GB1 fusionsDepositorTypeEmpty backboneUseTagsAvi - 8His-GB1ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAT9
Plasmid#112590PurposeUsed for expression of protein in E. coli as Thrombin cleavable N-termina 6His-tag-MBP fusions and without NcoI site within MBP.DepositorTypeEmpty backboneUseTags6His-MBPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLOCK.1.0-gDNA
Plasmid#112406PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CLOCKDepositorInsertCLOCK (CLOCK Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLOCK-donor
Plasmid#112310PurposeCRISPR donor plasmid to tag human transcription factor CLOCK with GFPDepositorInsertCLOCK homology arms flanking EGFP-IRES-Neo cassette (CLOCK Human)
UseCRISPRTagsExpressionMutationhg38:4:55435551:A>G, hg38:4:55435749:G>APromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFOXA3.1.0-gDNA
Plasmid#112417PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor FOXA3DepositorInsertFOXA3 (FOXA3 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His HA GNU WT
Plasmid#112994PurposeBaculovirus expression of His/HA-tagged Drosophila gnuDepositorInsertgnu (gnu Fly)
UseTagsHis/HAExpressionInsectMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
gRNA[bTub].1026C
Plasmid#112691Purposeexpress gRNA targeting bTub under dU6-3 promoterDepositorInsertU6.3-gRNA[bTub]
UseCRISPRTagsExpressionMutationPromoterdU6-3Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_Y357F-PolyA
Plasmid#112287PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) Y357F mutant _ corresponding to tyrosine 407 in this isoformDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationTyrosine 357 to Phenyalanine corresponding to tyr…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTGC-U6.2
Plasmid#112809PurposePCR template vector for amplifying gRNAcore-pU6.3 promoter fragmentDepositorInsertU6:2-gRNAcore
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only