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p7XC3H

Plasmid

#47065

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pJW1507

Plasmid

#62361

Purpose

pFA6a -mVenus-BirA::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

c-terminal mVenus-BirA tagging

Use

Yeast c-terminal tagging vector

Tags

mVenus-BirA

Expression

Mutation

L232H mutation compared to standard mVenus

Promoter

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-600uDelta13V

Plasmid

#18014

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A Delta13

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A in binding protein

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-30uDelta13V

Plasmid

#18015

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu D154A Delta13

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

mglB-D154A

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pJW1234

Plasmid

#62367

Purpose

pRS305 pPGK1-BirA::LEU2

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA (birA )

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-3.2mDelta13V

Plasmid

#18011

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A S112A Delta13

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A, S112A in binding protein

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pJW1505

Plasmid

#62359

Purpose

RPL16a HA-TEV-AVI pop-in/pop-out plasmid

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

RPL16A

Use

Yeast tagging

Tags

HA-TEV-AVI

Expression

Mutation

Promoter

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBXC3H

Plasmid

#47068

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-170nDelta13V

Plasmid

#18018

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16F Delta13

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

WT binding protein (no F16A)

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pERB260

Plasmid

#67763

Purpose

Expresses BICD-mCherry-eDHFR

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

BICD-mCherry-eDHFR

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pBXNH3

Plasmid

#47067

Purpose

FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/v5-DEST hGlut9

Plasmid

#18088

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

Glut9 (SLC2A9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only
pJW1504

Plasmid

#62358

Purpose

RPS2 HA-TEV-AVI pop-in/pop-out plasmid

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

RPS2 fragment

Use

Yeast targeting

Tags

HA-TEV-AVI

Expression

Mutation

Promoter

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1506

Plasmid

#62360

Purpose

RPL16b HA-TEV-AVI pop-in/pop-out plasmid

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

RPL16B

Use

Yeast targeting

Tags

HA-TEV-AVI

Expression

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pTLNXA7

Plasmid

#49033

Purpose

FX cloning Xenopus oocyte expression vector with SP6 promoter and N-terminal chicken alpha7 signal sequence

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Xenopus oocytes expression

Tags

chicken alpha7 signal sequence

Expression

Mutation

Promoter

SP6

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pJW1508

Plasmid

#62362

Purpose

pFA6a BirA-mVenus-SSH1::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-mVenus-Ssh1

Use

Tags

BirA-mVenus

Expression

Yeast

Mutation

Promoter

Gal1

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCLAP

Plasmid

#200786

Purpose

Cloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Type

Empty backbone

Use

Tags

CLIPf and SNAPf-6xHis-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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SH4-FRB-EBFP2

Plasmid

#222418

Purpose

To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al bioRxiv 2024.05.31.596797

Insert

SH4

Use

Tags

FRB-HA-EBFP2

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET_His-rsCaMPARI-mRuby3

Plasmid

#120804

Purpose

Expresses rsCaMPARI in bacterial cells

Depositor

Eric Schreiter

Article

Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

Insert

rsCaMPARI

Use

Tags

His-Xpress and mRuby3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1509

Plasmid

#62363

Purpose

pFA6a BirA-heh2 linker-SSH1::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-heh2-Ssh1

Use

Tags

Expression

Yeast

Mutation

Promoter

Gal1

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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