We narrowed to 1,571 results for: Tef;

1. pMTKM25

   Plasmid

   #233497

   Purpose

   Level 0 plasmid for MoClo, encodes the TEF3 promoter for K.marxianus

   Depositor

   Vincent Martin

   Article

   Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

   Insert

   promTEF3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICH47811_AvrSr62-7

   Plasmid

   #233534

   Purpose

   Expresses AvrSr62-7 in protoplasts

   Depositor

   Brande Wulff

   Article

   Chen et al bioRxiv 2024.08.30.610287

   Insert

   AvrSr62-7
   

   Use

   Protoplast expression

   Tags

   Luciferase

   Expression

   Plant

   Mutation

   Promoter

   Ubiquitin

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBKT7_AvrSr62-5

   Plasmid

   #233532

   Purpose

   Expresses AvrSr62-5 in yeast

   Depositor

   Brande Wulff

   Article

   Chen et al bioRxiv 2024.08.30.610287

   Insert

   AvrSr62-5
   

   Use

   Tags

   GAL4 BD, Myc tag

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBKT7_AvrSr62-1

   Plasmid

   #233531

   Purpose

   Expresses AvrSr62-1 in yeast

   Depositor

   Brande Wulff

   Article

   Chen et al bioRxiv 2024.08.30.610287

   Insert

   AvrSr62-1
   

   Use

   Tags

   GAL4 BD, Myc tag

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGADT7_Sr62NLRa

   Plasmid

   #233528

   Purpose

   Expresses Sr62NLRa in yeast

   Depositor

   Brande Wulff

   Article

   Chen et al bioRxiv 2024.08.30.610287

   Insert

   Sr62NLRa
   

   Use

   Tags

   GAL4 AD, HA tag

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWR76

   Plasmid

   #202776

   Purpose

   episomal fluorescent reporter plasmid for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   TDH3 promoter (S. stipitis)
   coCherry
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWR23

   Plasmid

   #202770

   Purpose

   episomal bioluminescent reporter plasmid for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coKanMX
   ACT1 terminator (S. stipitis)
   TDH3 promoter (S. stipitis)
   coCBG
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWR10

   Plasmid

   #202710

   Purpose

   episomal bioluminescent reporter plasmid for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coShBle
   ACT1 terminator (S. stipitis)
   TDH3 promoter (S. stipitis)
   coCBG
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWR9

   Plasmid

   #202645

   Purpose

   episomal bioluminescent reporter plasmid for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   TDH3 promoter (S. stipitis)
   coCBG
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWR54

    Plasmid

    #203330

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM11-6xHis-TEV-hORF1p_StammerAAA

    Plasmid

    #202587

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWR24

    Plasmid

    #202771

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coNAT
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWR16

    Plasmid

    #202711

    Purpose

    integrating bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    URA3 and flanking sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC19-sg-1

    Plasmid

    #190607

    Purpose

    Expresses a gRNA for base editing the EGFP Kozak sequence of pWPT-/mEGFP-1T-IRES-mCherry

    Depositor

    Alessandro Quattrone

    Article

    Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

    Insert

    gRNA sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-sgNCF1

    Plasmid

    #190193

    Purpose

    Expresses a gRNA for base editing the Kozak sequence of the human NCF1 gene

    Depositor

    Alessandro Quattrone

    Article

    Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

    Insert

    gRNA sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pILGFP8EFA

    Plasmid

    #185854

    Purpose

    Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    DHFR P66L

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. RIB ONLY R3

    Plasmid

    #161511

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_RIB1_R3_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting R3 in the RIB1 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RIB ONLY R4

    Plasmid

    #161512

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_RIB1_R4_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting R4 in the RIB1 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. THI ONLY pALD4 T1

    Plasmid

    #161498

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T1_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T1 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. THI ONLY pALD4 T2

    Plasmid

    #161499

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T2_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T2 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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