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Showing: 541 - 560 of 2271 results

  1. pMS73

    Plasmid
    #110629
    Purpose
    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d
    Inserts
    short U6 promoter
    cas9
    tnos terminator
    Use
    CRISPR; Self-replicating in ustilago maydis, conf…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Bacterial
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U. maydis hsp70 promoter (Kronstad and Leong, 198…
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110629-plasmid-map-sequence-id-210665

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  2. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Tight TRE promoter
    Available since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87360-plasmid-map-sequence-id-173092

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  3. pKP4010

    Plasmid
    #113336
    Purpose
    Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter
    Depositor
    Suzanne Sandmeyer
    Article
    Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do
    Inserts
    Artificial Transcription Factor (McIsaac et al, 2013)
    Ty3 Integrase
    Use
    Tags
    3X-FLAG/8X-Gly linker
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    113336-plasmid-map-sequence-id-217436

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  9. NbPT5b-p3

    Plasmid
    #160001
    Purpose
    Multigene construct containing the betalain pigment biosynthetic genes under the control of the NbPT5b promoter for visualisation of arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Inserts
    Bar (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Agrobacterium tumefaciens Nopaline synthase Promo…
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160001-plasmid-map-sequence-id-323057

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  10. NbBCP1b-p3

    Plasmid
    #160002
    Purpose
    Multigene construct containing the betalain pigment biosynthetic genes under the control of the NbBCP1b promoter for visualisation of arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Inserts
    Bar (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Agrobacterium tumefaciens Nopaline synthase Promo…
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160002-plasmid-map-sequence-id-323033

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  13. pInducer10b-EGFP-KRAS G12V

    Plasmid
    #164925
    Purpose
    Expresses EGFP cDNA and KRAS G12V cDNA in mammalian cells
    Depositor
    Ji Luo
    Article
    Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:
    Inserts
    Enhanced green fluorescent protein
    KRAS proto-oncogene, GTPase (KRAS) G12V mutant (KRAS Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Glycine 12 changed to valine (G->T 3156)
    Promoter
    Ubc Promoter and Ubc promoter
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164925-plasmid-map-sequence-id-333648

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  17. dCas9-p300 HAT

    Plasmid
    #179544
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179544-plasmid-map-sequence-id-354774

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  18. dCas9-CBP HAT

    Plasmid
    #179547
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179547-plasmid-map-sequence-id-354768

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  19. dCas9-GCN5 core

    Plasmid
    #179551
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179551-plasmid-map-sequence-id-354777

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  20. dCas9-p300 PHD-HAT

    Plasmid
    #179545
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179545-plasmid-map-sequence-id-354761

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Showing: 541 - 560 of 2271 results