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Plasmid#71827PurposeFor bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.DepositorInsertDnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
UseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
UseTags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-MinD_MinE
Plasmid#133621PurposeExpression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-CoilR-Cys
Plasmid#181989PurposeUsed to label proteins with the CoilE genetically encoded tagDepositorInsertCoilR-Cys
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherry-2xEcMTS
Plasmid#133624PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherry-2xEcMTS
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-Mff(1-61)-PP-GST
Plasmid#73042PurposeBacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage siteDepositorInsertMff(1-61) (Mff Mouse)
UseTagsGST-tag and PreScission protease siteExpressionBacterialMutationTruncation containing amino acids 1-61PromoterT7Available sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-SpyTag003
Plasmid#216282PurposeExpresses SUMO with a C-terminal SpyTag003 in bacterial cellsDepositorInsertSUMO-SpyTag003
UseTagsHis6 and SpyTag003ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-GFP-mHP1alpha
Plasmid#181912PurposeBacterial protein expression vector for GFP-tagged HP1alphaDepositorInsertmHP1alpha (Cbx5 Mouse)
UseTagsGFPExpressionBacterialMutationPromoterT7Available sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
VV001: WT Arch-3 in pET28b
Plasmid#58487PurposeExpresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coliDepositorInsertArchaerhodopsin-3 (with C-terminal His tag)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-IMX-DogTag
Plasmid#113765PurposeExpresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigaseDepositorInsertIMX-DogTag
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-L713F
Plasmid#174794PurposeBacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationL713F, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-C125G
Plasmid#174792PurposeBacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)DepositorInsertPARP1-C125G (PARP1 Human)
UseTags6xHisExpressionBacterialMutationC125G, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-iLOV-Rsn2
Plasmid#61687Purposebacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOVDepositorInsertRanaspumin-2
UseTagsHis and iLOVExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b-ETV6-ETS
Plasmid#111180PurposeMinimal ETS domain of human ETV6 (TEL)DepositorInsertETV6 ETS domain (ETV6 Bovine, Mouse, Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-miniR-DBCO
Plasmid#181984PurposeUsed to label proteins with the MiniE genetically encoded tagDepositorInsertMiniR-DBCO
UseTagsDBCO-Tag, His-TagExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-pro-sumo2
Plasmid#25102DepositorInsertpro-SUMO2 (SUMO2 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP[N150TAA]
Plasmid#164581Purposesuperfolder GFP with N150TAA mutationDepositorInsertsfGFP-150TAA
UseTags6xHisExpressionBacterialMutationN150TAA MutationPromoterT7Available sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-ART
Plasmid#174810PurposeBacterial expression of isolated PARP1 ART subdomainDepositorInsertPARP1-ART (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-BRCT
Plasmid#174807PurposeBacterial expression of isolated PARP1 BRCT domainDepositorInsertPARP1-BRCT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-WGR
Plasmid#174808PurposeBacterial expression of isolated PARP1 WGR domainDepositorInsertPARP1-WGR (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only