159,333 results

1. hBACCS2-IRES-mCherry

   Plasmid

   #72892

   Purpose

   Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherry

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   hBACCS2-IRES-mCherry
   

   Use

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-SLC38A9_STOP

   Plasmid

   #161236

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC38A9 (SLC38A9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. TPC2-G-GECO1.2

   Plasmid

   #207141

   Purpose

   Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   TPC2N2 (TPCN2 Human)
   

   Use

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFCiR2

   Plasmid

   #167512

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mRFP, Kanamycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CinRAM-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCin

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-Flag-Trib3

   Plasmid

   #131157

   Purpose

   Mammalian expression of flag-tagged mouse TRIB3

   Depositor

   Warren Pear, Karen Keeshan

   Article

   Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

   Insert

   Trib3 (Trib3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-pro-Il1b-C-Flag

   Plasmid

   #75131

   Purpose

   Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   interleukin 1 beta (Il1b Mouse)
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   (please see depositor comments below)

   Promoter

   CMV

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. hBACCS2

   Plasmid

   #67625

   Purpose

   Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   hBACCS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-EF1α-FRT-FLEX-GtACR1-EYFP

   Plasmid

   #114370

   Purpose

   expresses Flpo recombinase-dependent GtACR1

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   GtACR1-EYFP (NEWENTRY )
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTK511

   Plasmid

   #114686

   Purpose

   Express Mem-BFP-iLID

   Depositor

   Tomomi Kiyomitsu

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Insert

   Mem-BFP-iLID
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFRT-DHX36iso2

    Plasmid

    #159586

    Purpose

    Express N-ter FLAG HA tagged DHX36 isoform 2 in mammalian cells

    Depositor

    Markus Hafner

    Article

    Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

    Insert

    DHX36 (DHX36 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJH462

    Plasmid

    #172154

    Purpose

    pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para (Sumo-tagged pylBCD); AraC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    SUMO tag fused to N-terminus of PylB

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCh-Climp63

    Plasmid

    #136293

    Purpose

    expression of Climp63

    Depositor

    Gia Voeltz

    Article

    Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

    Insert

    Cytoskeletal-linking protein 63 (Ckap4 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQL47 EGFP-VAMP-GST

    Plasmid

    #122971

    Purpose

    Reporter of botulinum toxin B cleavage

    Depositor

    Chandra Tucker

    Article

    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

    Insert

    EGFP-VAMP3-GST
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b-12x

    Plasmid

    #61699

    Purpose

    Expresses PTDH mutant Opt12x

    Depositor

    Huimin Zhao

    Article

    Johannes et al Biotechnol Bioeng. 2007 Jan 1;96(1):18-2

    Insert

    Opt12x
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

15. mCherry-TFR-20

    Plasmid

    #55144

    Purpose

    Localization: Transferrin Receptor, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TFR (TFRC Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-µLight

    Plasmid

    #201225

    Purpose

    Express muLight biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    µLight
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS

    Plasmid

    #145268

    Purpose

    Mammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    RUNX2(+10A)-IDR (RUNX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-MS2-HA-HsRRP6_Z

    Plasmid

    #148131

    Purpose

    Mammalian Expression of HsRRP6

    Depositor

    Elisa Izaurralde

    Insert

    HsRRP6 (EXOSC10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2 silent mutations compared to the sequence of is…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pER-mScarlet3_N1

    Plasmid

    #189772

    Purpose

    Construct for labelling endoplasmic reticulum in mammalian cells using ER targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    ER-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30a-HS-Tn5

    Plasmid

    #127916

    Purpose

    Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.

    Depositor

    Silin Zhong

    Article

    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

    Insert

    Hyperstable Tn5
    

    Use

    Tags

    E coli elongation factor Ts and Mxe intein - Chit…

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    T7

    Available since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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