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pAAV-Fos-CreERT2

Plasmid

#194643

Purpose

Expresses Cre-ERT2 under the control of c-Fos promoter

Depositor

Jun-Hyeong Cho

Publication

Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

Insert

Fos-CreERT2

Use

AAV

Expression

Mammalian

Promoter

c-Fos

Available since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAGGS SARS-CoV-2 BA.4/5 Spike

Plasmid

#186031

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.4/5 Spike

Depositor

Marceline Côté

Publication

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BA.4/5 Spike (S )

Expression

Mammalian

Mutation

BA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

7 Articles

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GFP-PS9

Plasmid

#177944

Purpose

Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Publication

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

eGFP (ORF1ab )

Expression

Mammalian

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-Frankenbody-FLAG-mRuby2

Plasmid

#175294

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Publication

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mRuby2

Tags

mRuby2

Expression

Mammalian

Available since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

Plasmid

#138460

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

Depositor

Jian Xu

Publication

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-KRAB-IRES-zsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.eGFP.2A.wtTau

Plasmid

#140424

Purpose

AAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTau

Depositor

Bradley Hyman

Publication

Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

Insert

eGFP-2A-Tau (MAPT Human, Synthetic)

Use

AAV

Tags

eGFP

Promoter

CBA

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223 SARS-CoV-2 NSP14

Plasmid

#141267

Purpose

Gateway-compatible Entry vector, with insert of NSP14 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pLJC5-3XMyc-EGFP-PEX26

Plasmid

#139059

Purpose

Myc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Publication

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XMyc-EGFP-PEX26

Use

Lentiviral

Tags

Myc

Promoter

UBC

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-sp-sgRNA-RNF2_+41nick

Plasmid

#135958

Purpose

S. pyogenes sgRNA for +41 nick in RNF2

Depositor

David Liu

Publication

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_+41 nicking sgRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGL3basic luciferase and renilla_polyA

Plasmid

#128046

Purpose

pGL3 basic luciferase reporter with co-expression of renilla luciferase

Depositor

Oskar Laur

Type

Empty backbone

Expression

Mammalian

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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STING-V1

Plasmid

#124262

Purpose

C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

Depositor

Eric Schirmer

Insert

TMEM173 (STING1 Human)

Tags

Venus1

Expression

Mammalian

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLX401-INK4A

Plasmid

#121919

Purpose

Doxycycline-inducible expression of p16-INK4A product of CDKN2A

Depositor

William Hahn

Publication

Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

Insert

p16-INK4A (CDKN2A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE Tet ON

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Tom20-V5-FKBP-AP_pLX304

Plasmid

#120914

Purpose

Lentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membrane

Depositor

Alice Ting

Publication

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

Tom20-V5-FKBP-AP

Use

Lentiviral

Expression

Mammalian

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

Plasmid

#107943

Purpose

Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain

Tags

3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

Expression

Mammalian

Mutation

E174R, S542R, K548R and D908A

Promoter

CAG

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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APEX2-csGBP

Plasmid

#108874

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-csGBP

Expression

Mammalian

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AAV-U6-dgRNA-CAG-MPH

Plasmid

#106259

Purpose

Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

Depositor

Juan Belmonte

Publication

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

MS2-P65-HSF1 (HSF1 Human, Synthetic)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CAG

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-ZNF598-TEV-3xFLAG

Plasmid

#105690

Purpose

Expresses C terminally 3xFLAG tagged ZNF598 protein

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Insert

ZNF598 (ZNF598 Human)

Tags

TEV-3xFLAG

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Ca-FLARE (protease)

Plasmid

#92214

Purpose

protease component of FLARE

Depositor

Alice Ting

Publication

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

CaM-V5-TEVp(Δ220-242)

Use

AAV

Expression

Mammalian

Promoter

synapsin

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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LZ10 PBREBAC-H2BHalo

Plasmid

#91564

Purpose

piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.

Depositor

Zhe J. Liu

Publication

Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1

Insert

histone H2B-HaloTag

Tags

HaloTag

Expression

Mammalian

Promoter

EF1

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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GFP-Rab27B

Plasmid

#89447

Purpose

Expression of GFP-tagged human Rab27B in mammalian cells

Depositor

Wendy Westbroek

Publication

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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