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pSin-EF2-Oct4-Pur

Plasmid

#16579

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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ColorfulCell

Plasmid

#62449

Purpose

MXS_chaining vector with 6 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::GTS-Citrine-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::NLS-TagBFPx3-bGHpA
CMV::MTS-AzamiGreen-bGHpA
CMV::iRFP670-SKL-bGHpA
CMV::ETS-mCherry-KDEL-bGHpA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-GFP-OSBP

Plasmid

#134659

Purpose

Flag and GFP-tagged OSBP

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

OSBP (OSBP Human)

Use

Lentiviral

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-GCN5

Plasmid

#65386

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

GCN5 (KAT2A Human)

Use

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV_mGL_PP7

Plasmid

#232440

Purpose

expression plasmid to produce mGreenLantern mRNA with a PP7 aptamer in the 3' UTR

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

Insert

mGreenLantern

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRck_AC

Plasmid

#148417

Purpose

Mammalian Expression of HsRck

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a mScarlet

Plasmid

#178543

Purpose

The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mScarlet

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pUAS-V5-TurboID-NES

Plasmid

#116904

Purpose

Expresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

Depositor

Norbert Perrimon

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pJYS1Ptac

Plasmid

#85545

Purpose

Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

Depositor

Sheng Yang

Article

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Inserts

Cpf1
recT

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Tags

Expression

Bacterial

Mutation

Promoter

tac and unknown

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pSIN-TRE-H2BeGFP-rtTA2

Plasmid

#165494

Purpose

Doxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.

Depositor

Hector G. Palmer

Article

Puig et al J Clin Invest. 2018 Aug 31;128(9):3887-3

Insert

Human histone H2B (H2BC21 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

-

Promoter

TetO-CMV

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pM1s3AsG

Plasmid

#137921

Purpose

E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression

Depositor

Neel Joshi

Article

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Insert

Superfolder GFP

Use

Synthetic Biology; For use with e. coli nissle in…

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-sgRNA expression vector

Plasmid

#51132

Purpose

For in vitro transcription of sgRNA from the T7 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-CAAX

Plasmid

#86056

Purpose

A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Homo sapiens Harvey rat sarcoma (HRAS Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains the region from 825-857 of HRAS as a far…

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD-Flp

Plasmid

#122969

Purpose

Expresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.

Depositor

Lydia Freddolino

Article

Scholz et al Cell Syst. 2019 Mar 27;8(3):212-225.e9.

Insert

Flp recombinase

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Aug. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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LeGO-Cer2

Plasmid

#27338

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, Cerulean

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-RFP

Plasmid

#22910

Depositor

Edward Callaway

Article

Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

Insert

Chicken Beta-actin promoter driving DsRedExpress

Use

AAV; Adeno-associated virus

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-3xFLAG-hphMX4

Plasmid

#20755

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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Cherry-ICP0-C3

Plasmid

#134560

Purpose

Expresses Cherry-ICP0 fusion protein

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

ICP0

Use

Tags

Cherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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SIRT3 Flag

Plasmid

#13814

Purpose

Mammalian expression of human SIRT3 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT3 (SIRT3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pENTR TERRA

Plasmid

#111383

Purpose

TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

Depositor

Klaus Holzmann

Article

Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

Insert

TERRA

Use

Gateway entry cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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