19,654 results

1. AICSDP-7:SEC61B-mEGFP

   Plasmid

   #87426

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   SEC61B Homology Arms with mEGFP-linker (SEC61B Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pUB_smHA_KDM5B_MS2

   Plasmid

   #81085

   Purpose

   Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).

   Depositor

   Tim Stasevich

   Article

   Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

   Insert

   smHA_KDM5B_24xMS2
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMpGE013

   Plasmid

   #108681

   Purpose

   All-in-one genome editing vector in M. polymorpha (hygromycin resistant)

   Depositor

   Takayuki Kohchi, Shigeo Sugano

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti6.2-3xFLAG-V5-ccdB

   Plasmid

   #87072

   Purpose

   Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

   Depositor

   Susan Lindquist, Mikko Taipale

   Article

   Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PPRE-H2B-eGFP

   Plasmid

   #84393

   Purpose

   Fluorescent reporter for PPARgamma activity

   Depositor

   Severine Degrelle, Thierry Fournier

   Article

   Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

   Insert

   PPARG response element (PPARG Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   PPRE

   Available Since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CD44P pGL3

   Plasmid

   #19122

   Depositor

   Bob Weinberg

   Article

   Godar et al Cell. 2008 Jul 11. 134(1):62-73.

   Insert

   CD44 promoter (CD44 Human)
   

   Use

   Non viral cloning vector

   Available Since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ARHGEF11(DHPH)-CRY2-mCherry

   Plasmid

   #89481

   Purpose

   Also known as optoGEF-RhoA, encodes for a fusion between CRY2(1-498)-mCherry and the DHPH domain of ARHGEF11

   Depositor

   Xavier Trepat Guixer

   Article

   Valon et al Nat Commun. 2017 Feb 10;8:14396. doi: 10

   Insert

   ARHGEF11(DHPH) - CRY2(1-498) - mCherry (CRY2, ARHGEF11 Mustard Weed, Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-JIP3

   Plasmid

   #164624

   Purpose

   Expresses JIP3 with a GFP tag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   JIP3 (MAPK8IP3 Human)
   

   Tags

   cycle 3 GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1_crGE

   Plasmid

   #172933

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GPS-MYC

    Plasmid

    #160130

    Purpose

    A reporter that monitors MYC protein expression.

    Depositor

    Channing Der

    Article

    Blake et al Sci Signal. 2019 Jul 16;12(590). pii: 12

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmScarlet_Giantin_C1

    Plasmid

    #85048

    Purpose

    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Giantin (GOLGB1 Human)
    

    Expression

    Mammalian

    Mutation

    aa 3131-3259

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRN114

    Plasmid

    #127221

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, Wus, AtSTM
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-Clover

    Plasmid

    #40259

    Purpose

    GFP with robust performance in protein fusions and useful as FRET donor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Insert

    Clover GFP
    

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Sept. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT

    Plasmid

    #87259

    Purpose

    YFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133

    Depositor

    David Sibley

    Article

    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

    Inserts

    YFP-mAID-3HA
    HXGPRT
    

    Use

    Toxoplasma expression

    Tags

    3HA and mAID

    Mutation

    Codon optimized for Toxoplasma gondii expression

    Promoter

    TgDHFR-TS 463 bp and TgTUB1 378 bp

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-N1-IL6

    Plasmid

    #111933

    Purpose

    Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

    Insert

    Interleukin 6 (IL6 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells

    Promoter

    CMV

    Available Since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. C1(1-29)-miniTurbo-V5_pCDNA3

    Plasmid

    #107174

    Purpose

    expresses V5-tagged miniTurbo on the mammalian ER membrane

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Synapsin-cyto-iATPSnFR1.0

    Plasmid

    #102556

    Purpose

    expresses cytoplasmic ATP sensor in neurons

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Use

    AAV

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

    Plasmid

    #87306

    Purpose

    An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

    Insert

    Cre-dependent mouse codon-optimized Flp recombinase.
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCIP-Flag-TfebAA

    Plasmid

    #79014

    Purpose

    For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Tfeb (Tfeb Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S142A, S211A

    Promoter

    pCAGGs

    Available Since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 2C-3XtbGFP-PEST

    Plasmid

    #69072

    Purpose

    Expresses 3XturboGFP-NLS-PEST under MERVL_LTR

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

    Insert

    turboGFP
    

    Expression

    Mammalian

    Available Since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge