We narrowed to 28,440 results for: REP

1. pMJ915

   Plasmid

   #69090

   Purpose

   Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

   Depositor

   Jennifer Doudna

   Article

   Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

   Insert

   cas9
   

   Use

   Tags

   MBP, hexa His, and two SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMP11

   Plasmid

   #215559

   Purpose

   pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Inserts

   Cas9
   gRNA targeting pBR322 origin of replication
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23105 and tet

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMA3287

   Plasmid

   #46883

   Purpose

   Lentiviral vector encoding Tet-inducible mitochondrially targeted Y147A mutant human Uracil-N-glycosylase

   Depositor

   Mikhail Alexeyev

   Article

   Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

   Insert

   Uracil-N-glycosylase, Y147A mutant (UNG Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Y147A

   Promoter

   TRE-Tight

   Available since

   Aug. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTU1-A-SP44-sfGFP

   Plasmid

   #163757

   Purpose

   Expresses Streptomyces codon-optimised sfGFP

   Depositor

   Simon Moore, Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   SP44

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFB1_hPMS2

   Plasmid

   #129425

   Purpose

   Expression human PMS2 protein using baculovirus

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Insert

   human PMS2 (PMS2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcGFP-MALAT1M

   Plasmid

   #120382

   Purpose

   Reporter for RNA nuclear localization

   Depositor

   Igor Ulitsky

   Article

   Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

   Insert

   MALAT1 (MALAT1 Human)
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFB1_hMLH1

   Plasmid

   #129426

   Purpose

   Expression human MLH1 protein using baculovirus

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Insert

   human MLH1 (MLH1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. hSyn1-sypHy-RGECO

   Plasmid

   #84078

   Purpose

   Combined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosis

   Depositor

   Juan Burrone

   Article

   Jackson et al Front Synaptic Neurosci. 2016 Jul 26;8:2

   Insert

   sypHy-RGECO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCARS1

   Plasmid

   #174868

   Purpose

   Contains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1

   Depositor

   Zengyi Shao

   Article

   Lopez et al Appl Microbiol Biotechnol. 2021 Aug 6. p

   Insert

   ARS1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTcI39-switch

    Plasmid

    #195557

    Purpose

    Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mRFP1
    mWasabi
    TcI39
    

    Use

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. psiCHECK-2-Smad2-3'UTR

    Plasmid

    #61775

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Smad2

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Smad2 3'UTR
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

12. psiCHECK-2-Smad5-3'UTR

    Plasmid

    #61777

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Smad5

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Smad5 3'UTR
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCRISPEY-GAL-Kan

    Plasmid

    #232099

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPEY-GAL-Hyg

    Plasmid

    #232098

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFB1_hMSH2

    Plasmid

    #129423

    Purpose

    Expression human MSH2 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MSH2 (MSH2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. 8xNFAT-ZsG-mCD8

    Plasmid

    #162747

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTU1-A-SP44-mScarlet-I

    Plasmid

    #163756

    Purpose

    Expresses Streptomyces codon-optimised mScarlet-I

    Depositor

    Simon Moore, Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Insert

    mScarlet-I
    

    Use

    Synthetic Biology

    Tags

    Flag and FlAsH tags

    Expression

    Bacterial

    Mutation

    Promoter

    SP44

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. GCaMP-AMPK-SPARK

    Plasmid

    #226257

    Purpose

    Dual reporter of Ca2+ levels and AMPK activity

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    GCaMP-AMPK-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pmCherry_gRNA

    Plasmid

    #80457

    Purpose

    gRNA expression vector with mCherry transfection control

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Inserts

    sp Cas9 gRNA
    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken β-actin promoter

    Available since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. CJ8421_04975 Rptr2 and CJ8421_04975 mRNA

    Plasmid

    #194210

    Purpose

    Expresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    CJ8421_04975 mRNA and CJ8421_04975 Rptr2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. tracrRNA(scr)-CJ8421_04975 mRNA

    Plasmid

    #199718

    Purpose

    Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    CJ8421_04975 mRNA and scrambled tracrRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHD-DsRed

    Plasmid

    #51434

    Purpose

    Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.

    Depositor

    Kate O'Connor-Giles

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Insert

    LoxP-3xP3-DsRed-LoxP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    3xP3

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pRPA70-IRES-Ven-RPA32-IRES-RPA14

    Plasmid

    #211430

    Purpose

    mammalian expression of human RPA32 N-terminally fused to mVenus and RPA70 and RPA14

    Depositor

    Helmut Pospiech

    Insert

    RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)
    

    Use

    Tags

    mVenus (on RPA32)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. 8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2

    Plasmid

    #84603

    Purpose

    Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

    Depositor

    James Chen

    Article

    Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

    Insert

    mCherry
    

    Use

    Zebrafish transgenesis

    Tags

    ODC1 destabilizing element

    Expression

    Mutation

    Promoter

    8xGliBS-delta-crystallin minimal promoter

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pMXs-Ms-c-Myc-W136E

    Plasmid

    #26024

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

    Insert

    c-Myc (Myc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    W136E

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pThermeleon

    Plasmid

    #86120

    Purpose

    Bandpass temperature-gated expression of mWasabi and mCherry reporters

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    TcI38
    mCherry
    cI
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…

    Promoter

    pR/pL, pR/pL, pLacI, and pTlpA

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCas3

    Plasmid

    #229538

    Purpose

    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Insert

    Cas3
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. FL-mCherry

    Plasmid

    #78862

    Purpose

    DULIP negative control (prey) to exclude unspecific background binding

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Insert

    mCherry
    

    Use

    Expression vector

    Tags

    Firefly luciferase, V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. pKLBac-FluB-trimer

    Plasmid

    #229859

    Purpose

    Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

    Depositor

    Stephen Cusack

    Article

    Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

    Inserts

    PA, Influenza B (B/Memphis/13/03)
    PB1, Influenza B (B/Memphis/13/03)
    PB2, Influenza B (B/Los Angeles/1/02)
    

    Use

    Tags

    2x-Streptag and 8xHis-tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. Plasmid L5 attB::Pleft* mCherry

    Plasmid

    #169411

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mCherry
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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