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pAc5.1B-EGFP-DmDCP1_327-372_H

Plasmid

#146500

Purpose

Insect Expression of DmDCP1_327-372

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_327-372 (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-As-NF-kB1

Plasmid

#180209

Purpose

Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB1 protein in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB1

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 2-409

Promoter

AdhI

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-As-NF-kB2

Plasmid

#180210

Purpose

Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB2 protein in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB2

Tags

GAL4 (aa 1-147) fusion protein

Expression

Yeast

Mutation

aa 2-502

Promoter

AdhI

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-As-NF-kB3

Plasmid

#180211

Purpose

Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB3 protein in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB3

Tags

GAL4 (aa 1-147) fusion protein

Expression

Yeast

Mutation

aa 2-412

Promoter

AdhI

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Co-NF-kB-Cterm

Plasmid

#180199

Purpose

Expresses FLAG-tagged Capsaspora NF-kB C-terminal sequences in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-NF-kBCterm

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 542-1224

Promoter

CMV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCup_1-417-V5His6_C

Plasmid

#146028

Purpose

Insect Expression of DmCup_1-417

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_1-417 (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCup_419-771-V5His6_C

Plasmid

#146029

Purpose

Insect Expression of DmCup_419-771

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_419-771 (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup-4Ala-mut_C

Plasmid

#146031

Purpose

Insect Expression of DmCup-4Ala-mut

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup-4Ala-mut (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_1-417-V5His6_C

Plasmid

#146032

Purpose

Insect Expression of DmCup_1-417

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_1-417 (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_419-771-V5His6_C

Plasmid

#146033

Purpose

Insect Expression of DmCup_419-771

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_419-771 (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP1_1-127_A

Plasmid

#145901

Purpose

Insect Expression of DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_1-127 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP1_128-372_A

Plasmid

#145902

Purpose

Insect Expression of DmDCP1_128-372

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_128-372 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmDCP1_1-127_A

Plasmid

#145903

Purpose

Insect Expression of DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_1-127 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmDCP1_128-372_A

Plasmid

#145904

Purpose

Insect Expression of DmDCP1_128-372

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_128-372 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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MSNA-c029

Plasmid

#175471

Purpose

Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R and H288A point mutations that reduce binding to CD44.

Depositor

Opher Gileadi

Article

Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

Insert

MSN (MSN Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

L281R, H288A

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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MSNA-c028

Plasmid

#175470

Purpose

Protein expression in bacterial cells. FERM domain, M1-E346. Contains H288A point mutation that reduces binding to CD44.

Depositor

Opher Gileadi

Article

Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

Insert

MSN (MSN Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

H288A

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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MSNA-c021

Plasmid

#175468

Purpose

Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R point mutation that reduces binding to CD44.

Depositor

Opher Gileadi

Article

Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

Insert

MSN (MSN Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

L281R

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

Plasmid

#176575

Purpose

Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Avitag, Hexahistidine tag, and mEGFP

Expression

Bacterial

Mutation

E184Q W330Y

Promoter

T7

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin-FLN-ELP-His-ybbr

Plasmid

#168039

Purpose

Expresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc227

Plasmid

#161948

Purpose

Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on TetR

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter

Expression

Bacterial

Promoter

PBAD

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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