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pGL403

Plasmid

#119953

Purpose

Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

Depositor

Nigel Scrutton

Publication

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

gpps
limS

Expression

Bacterial

Mutation

N-terminal truncation of 56 amino acid residues (…

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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StrKDEL-IRES_mScarleti_Sec23A

Plasmid

#117273

Purpose

Expresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23A

Depositor

David Stephens

Publication

McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

SEC23A (SEC23A Human)
Streptavidin-KDEL

Tags

mScarlet-i

Expression

Mammalian

Mutation

Human Sec23A; Sequencing showed a mutation in Sec…

Promoter

CMV and IRES

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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AAEL006511-Cas9

Plasmid

#100580

Purpose

The promoter of AAEL006511 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Publication

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL006511-hspcas9-GFP
Opie2-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL006511 and Opie2

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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DNA-CARzeta-IRESpuro

Plasmid

#89345

Purpose

2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassette

Depositor

Ron Vale

Publication

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

DNA-CARzeta

Use

Lentiviral

Tags

SNAPf tag

Expression

Mammalian

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pTE4254

Plasmid

#89050

Purpose

Expresses St chimeric gRNA and human codon-optimized StCas9

Depositor

Ervin Welker

Publication

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

St chimeric gRNA
hStCas9

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV and human U6

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry GRK2 ct in pcDNA3.1

Plasmid

#64212

Purpose

mCherry fused between Cry2 and GRK2ct to study cell migration

Depositor

Narasimhan Gautam

Publication

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK2 ct (GRK2 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV and cmv

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only
pDONR-221-P3-P4

Plasmid

#186351

Purpose

Gateway donor vector with AttP3 AttP4 recombination sites

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAB150

Plasmid

#101661

Purpose

Opto-T7RNAP*(563)

Depositor

Mustafa Khammash

Publication

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563)

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDSG287

Plasmid

#115606

Purpose

surface-displayed Ag2, used in composability experiments; low copy

Depositor

Ingmar Riedel-Kruse

Publication

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAYC

Plasmid

#62657

Purpose

Mammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Publication

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

iCre

Expression

Mammalian

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-U6-gRNA-TnT-Cre

Plasmid

#132551

Purpose

For loss-of-function experiments in cardiomyocytes

Depositor

William Pu

Inserts

TnT-Cre
U6-gRNA

Use

AAV

Promoter

TnT and U6

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCXLE-hS + eGFP

Plasmid

#74945

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Publication

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sox2 (SOX2 Human)

Expression

Mammalian

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only
pCAG:ECFP

Plasmid

#32597

Depositor

Anna-Katerina Hadjantonakis

Publication

Hadjantonakis et al BMC Biotechnol. 2002 Jun 11;2:11.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ECFP

Expression

Mammalian

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBPHLWL

Plasmid

#26254

Depositor

Tom Clandinin

Publication

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

Multiple cloning site

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pTK332

Plasmid

#59573

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5

Depositor

Ron Weiss

Publication

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG:EYFP

Plasmid

#32596

Depositor

Anna-Katerina Hadjantonakis

Publication

Hadjantonakis et al BMC Biotechnol. 2002 Jun 11;2:11.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EYFP

Expression

Mammalian

Promoter

pCAGGs

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only
pEntry_HCoV-HKU1-Spike

Plasmid

#168911

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-Spike (S )

Use

Gateway Cloning

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pWPI-FLAG-hPRKCI

Plasmid

#35387

Depositor

Luke McCaffrey

Insert

Protein Kinase C iota (PRKCI Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available since

June 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pICH47802

Plasmid

#48007

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pAAV-hSyn-miniDq-P2A-mCherry

Plasmid

#204357

Purpose

AAV vector for miniDq expression under the control of human synapsin promoter

Depositor

Masao Doi

Publication

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDq-P2A-mCherry

Use

AAV

Tags

mCherry

Mutation

The third intracellular loop (ICL3) of hM3Dq was …

Promoter

hSyn

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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