Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 11,999 results for: SHA

Showing: 8401 - 8430 of 11999 results

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pDB.His.MBP.3C NLRP1 UPA-CARD W1460A

Plasmid

#164006

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

W1460A

Available Since

Feb. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C NLRP1 UPA-CARD M1457A

Plasmid

#164005

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

M1457A

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C NLRP1 UPA-CARD Y1445A

Plasmid

#164004

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

Y1445A

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG W1460A

Plasmid

#163989

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

W1460A

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG Y1445A

Plasmid

#163987

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Y1445A

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1427E

Plasmid

#163986

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

R1427E

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1414R

Plasmid

#163985

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E1414R

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1411R

Plasmid

#163984

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E1411R

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG D1401R

Plasmid

#163983

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

R1401R

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-dt/CBA-EGFP-R106bT

Plasmid

#163921

Purpose

Serves as control to pLV-PGK-dt/CBA-EGFP-106bT.

Depositor

John Engelhardt

Article

Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

Insert

dtomato and EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

Added 4-tandem reverse orientation-mir-106b-targe…

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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p1A Prk-

Plasmid

#162694

Purpose

p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

H. neapolitanus CbbLS and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Mutation

Prk inactive (K20M S21A native protein, this map …

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1397R

Plasmid

#163982

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E1397R

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1392E

Plasmid

#163981

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

R1392E

Available Since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1386E

Plasmid

#163980

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

R1386E

Available Since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Ala148

Plasmid

#158630

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Mutation

Q148A

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-HOXB4

Plasmid

#139829

Purpose

doxycycline-inducible expression of Human HOXB4 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

HOXB4 (HOXA6 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R249P- Please see depositor comment below

Promoter

TRE-mCMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-KLF4

Plasmid

#139828

Purpose

doxycycline-inducible expression of Human KLF4 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

KLF4 (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Bod1l_Sense

Plasmid

#124443

Purpose

Plasmid for sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

Biorientation of chromosomes in cell division 1-like (Bod1l Mouse)

Use

In situ probe

Promoter

T7

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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DLG3_PDZ_2

Plasmid

#103947

Purpose

Protein expression and purification of NE-DLG PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

DLG3 (DLG3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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MAGI1_PDZ_1

Plasmid

#103898

Purpose

Protein expression and purification of MAGI1 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MAGI1 (MAGI1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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MAGI1_PDZ_4

Plasmid

#103901

Purpose

Protein expression and purification of MAGI1 PDZ4 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MAGI1 (MAGI1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 T78R L84R T92R V118R

Plasmid

#112106

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T78R L84R T92R V118R

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500

Plasmid

#92315

Purpose

Expression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

B1 domain of protein L (PL) mutant K16TAG

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70b (p70a promoter with one mutation)

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBEST-p70b-UTR1-NTerm 6xHis-zmADH-T500

Plasmid

#92229

Purpose

Expression of zmADH using the p70b (weak version of the p70a promoter) promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

NTerm 6xHis Tagged zmADH

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70b (p70a promoter with one mutation)

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
csd6_17-330/pET28b(+) N-tag

Plasmid

#71353

Purpose

csd6, construct:17-330, pET28b(+) N-tag (Please note that this plasmid has a C-terminal His tag)

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_E110A/pET28b(+) N-tag

Plasmid

#71354

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:E110A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Glutamic acid 110 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_Y133A/pET28b(+) N-tag

Plasmid

#71356

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:Y133A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Tyrosine 133 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_C176A/pET28b(+) N-tag

Plasmid

#71360

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:C176A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Cysteine 176 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_H160A/pET28b(+) N-tag

Plasmid

#71359

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:H160A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Histidine 160 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_W158A/pET28b(+) N-tag

Plasmid

#71358

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:W158A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Tryptophan 158 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Showing: 8401 - 8430 of 11999 results

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