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pAP1836

Plasmid

#137087

Purpose

Plasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherry

Depositor

Geraldine Seydoux

Article

Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

Insert

mCherry

Use

Pcr template for donor dna

Expression

Bacterial

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLMB686

Plasmid

#83780

Purpose

myo-Inositol biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

upstream region of RL4655 (intA)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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p alpha-actinin:GAL4/UAS:TdTomato

Plasmid

#161712

Purpose

Express TdTomato through amplification of GAL4/UAS system

Depositor

Hollis Cline

Article

Hiramoto et al Proc Natl Acad Sci U S A. 2014 Nov 25;11

Insert

TdTomato, GAL4

Use

Xenopus laevis expression

Tags

TdTomato

Expression

Mammalian

Promoter

actin-GAL4, UAS

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3_AKAR5Δ

Plasmid

#119912

Purpose

Mammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

AKAR5Δ

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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PcDNA-DEST47-CypB-Iso C-term GFP

Plasmid

#36130

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 47-204 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST47-CypB-Golgi C-term GFP

Plasmid

#36128

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1-211 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST53-CypB-Golgi N-term GFP

Plasmid

#36127

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1-211 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pNC1137

Plasmid

#41563

Depositor

Beverly Errede

Article

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

Ura3-FUS1-UBIMdkGFP*-SpHis5-Tim9

Mutation

GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pAH35 (Pmyo-2::cGAL-N::let-858 3'UTR)

Plasmid

#107134

Purpose

cGAL-N driver under the control of C. elegans myo-2 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein

Expression

Worm

Promoter

C. elegans myo-2 promoter

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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VV221: sdChR(C138S)-TS-eGFP-ER in fck

Plasmid

#58513

Purpose

Expresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S)-TS-eGFP-ER

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Glt1b

Plasmid

#98706

Purpose

mammalian expression of Glt1b

Depositor

Paul Rosenberg

Article

Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

Insert

Glt1b (Slc1a2 Rat)

Expression

Mammalian

Mutation

I348V and N365Y (please see depositor comments se…

Promoter

CMV

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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PcDNA-DEST47-CypB-Nucl2 C-term GFP

Plasmid

#36126

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains CypB AA 34-211

Promoter

CMV

Available Since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
pP2AeGFP-Mars1Cy

Plasmid

#75223

Purpose

Mammalian intracellular expression of fluorogen activating protein Mars1Cy and eGFP

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

EGFP-p2a-Mars1Cy

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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GST-PRMT7 LDIG69AAAA

Plasmid

#61560

Purpose

Hs GST-PRMT7 LDIG(69-72)AAAA AdoMet binding deficient mutant

Depositor

Steven Clarke

Article

Feng et al J Biol Chem. 2014 Nov 21;289(47):32604-1

Insert

PRMT7 (PRMT7 Human)

Tags

GST

Expression

Bacterial

Mutation

LDIG 69-72 AAAA

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST47-CypB-Nucl C-term GFP

Plasmid

#36124

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

Cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 34-216 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST47-CypB-Del2 C-term GFP

Plasmid

#36134

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 38-216 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pRK5_tAKARγ

Plasmid

#119915

Purpose

Mammalian expression of the tAKARγ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARγ

Expression

Mammalian

Available Since

April 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1α-ancestral-retroCHMP3-HA

Plasmid

#154248

Purpose

expresses ancestral New World monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed ancestral sequence of New World monkey retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α-owl-monkey-retroCHMP3-HA

Plasmid

#154249

Purpose

expresses owl monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pJL6

Plasmid

#184134

Purpose

For labelling (RFP) the apical region of the intestine. Translational fusion construct. coel::RFP_Pvha-6::vha-6::dsred.

Depositor

Alejandro Aballay

Article

Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

Insert

CELE_VW02B12L.1 (vha-6 Nematode)

Tags

RFP

Expression

Worm

Promoter

Pvha-6 (894bp)

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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